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The map label for this gene is gadW

Identifier: 157162993

GI number: 157162993

Start: 3705201

End: 3705929

Strand: Reverse

Name: gadW

Synonym: EcHS_A3717

Alternate gene names: 157162993

Gene position: 3705929-3705201 (Counterclockwise)

Preceding gene: 157162994

Following gene: 157162988

Centisome position: 79.81

GC content: 41.84

Gene sequence:

>729_bases
ATGACTCATGTCTGCTCGGTGATCCTCATTCGTCGTTCATTCGATATTTATCATGAACAGCAAAAAATATCGCTGCATAA
CGAGAGTATTCTGCTGCTGGAGAAAAATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCG
ATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTG
TTAATTAACCAGTCACCCCCCATGCCGCTGGTGACGGCGATTTTTGATAGCTTCAATGAATCCGGGGTAAATTCACCGAT
ACTGAGCAATATGCTCTACCTTTCCTGTTTATCGATGTTTTCTCATAAGAAAGAACTGATCCCCTTACTTTTCAATAGCA
TCAGCACTGTTTCAGGAAAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGAA
AGAATGTATACCAGCGAGAGTCTAATCAAAAAAAAGTTGCAGGATGAAAATACCTGTTTCAGTAAAATATTACTCGCCTC
CAGGATGTCGATGGCCAGACGATTACTCGAGTTACGTCAAATTCCTCTGCATACTATTGCGGAAAAATGTGGCTATAGCA
GTACATCGTACTTTATAAACACATTTCGACAATATTATGGTGTAACGCCACATCAGTTTGCGCAACATTCGCCAGGTACC
TTTTCCTGA

Upstream 100 bases:

>100_bases
CTACCAAATCTGGCAGTTTTTGCGCTAAGAAACAGTCTGGCATCATTTCATTAGTATACTGAAATTGAAATAATCGCAGT
ATGAAATATAAGGGATAATC

Downstream 100 bases:

>100_bases
CATATTTCGCATTTGAATATTGGTCAGGATCTCACACCTGCTTCATGTGAAACTCTTCCCTGATGATTTCTGCCGGGCTA
CCGGCTAGTTCTCTTTCGCA

Product: transcriptional regulator GadW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS

Sequences:

>Translated_242_residues
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS
>Mature_241_residues
THVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLL
INQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGTF
S

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789932, Length=242, Percent_Identity=100, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1786776, Length=241, Percent_Identity=35.6846473029046, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=34.1772151898734, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789933, Length=209, Percent_Identity=32.5358851674641, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790557, Length=133, Percent_Identity=37.593984962406, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786778, Length=124, Percent_Identity=41.1290322580645, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): GADW_ECO57 (P63202)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91178
- PIR:   D86024
- RefSeq:   NP_290095.1
- RefSeq:   NP_312422.1
- ProteinModelPortal:   P63202
- SMR:   P63202
- EnsemblBacteria:   EBESCT00000025964
- EnsemblBacteria:   EBESCT00000059915
- GeneID:   915748
- GeneID:   961148
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4928
- KEGG:   ecs:ECs4395
- GeneTree:   EBGT00050000008641
- HOGENOM:   HBG467631
- OMA:   INQLPPM
- ProtClustDB:   CLSK880699
- BioCyc:   ECOL83334:ECS4395-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 28029; Mature: 27898

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC
>Mature Secondary Structure 
THVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCH
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796