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The map label for this gene is uxaA [H]

Identifier: 157162568

GI number: 157162568

Start: 3285380

End: 3286867

Strand: Reverse

Name: uxaA [H]

Synonym: EcHS_A3274

Alternate gene names: 157162568

Gene position: 3286867-3285380 (Counterclockwise)

Preceding gene: 157162569

Following gene: 157162567

Centisome position: 70.78

GC content: 53.76

Gene sequence:

>1488_bases
ATGCAATACATCAAGATCCATGCGCTGGATAACGTCGCGGTCGCTTTAGCGGATTTGGCTGAAGGCACAGAAGTCAGTGT
CGATAACCAAACTGTTAGGCTGCGCCAGGATGTTGCTCGTGGACATAAATTTGCGTTAACGAATATCGCAAAAGGGGCCA
ACGTCATCAAATATGGCCTGCCGATTGGTTATGCATTGGCGGATATTGCGGCGGGGGAACACGTTCACGCCCACAATACG
CGCACGAATCTGAGCGATCTGGATCAGTATCGCTATCAACCTGATTTTCAGGATCTGCCTGCGCAAGCGGCAGATCGTGA
AGTGCAGATCTATCGTCGCGCTAACGGCGATGTCGGGGTGCGTAATGAGCTGTGGATCCTGCCAACCGTGGGCTGTGTCA
ACGGCATCGCGCGGCAGATCCAAAATCGTTTCCTGAAAGAAACCAACAACGCCGAAGGTACCGACGGCGTGTTCCTCTTC
AGCCACACCTACGGCTGCTCACAGCTGGGCGACGATCACATTAATACCCGCACCATGCTGCAAAACATGGTGCGCCACCC
GAACGCAGGCGCAGTGCTGGTGATTGGTCTGGGCTGTGAAAACAACCAGGTTGCCGCATTCCGTGAAACGCTGGGCGATA
TCGATCCTGAACGCGTTCATTTCATGATCTGCCAACAGCAGGATGATGAGATCGAAGCCGGAATCGAGCATTTGCATCAG
CTGTATAACGTGATGCGCAACGATAAACGCGAGCCAGGCAAACTCAGCGAACTGAAGTTTGGTCTGGAGTGCGGTGGTTC
TGACGGTCTTTCTGGTATTACTGCTAACCCGATGCTGGGGCGTTTCTCTGACTACGTGATTGCTAACGGCGGTACTACCG
TACTGACCGAAGTGCCGGAGATGTTTGGCGCAGAGCAGTTGCTGATGGACCATTGCCGCGACGAAGCAACGTTTGAAAAA
CTGGTCACCATGGTCAACGACTTCAAACAGTACTTTATTGCCCATGATCAGCCGATCTATGAAAACCCATCGCCGGGGAA
CAAAGCGGGCGGTATCACCACGCTGGAAGACAAATCACTTGGCTGTACCCAGAAAGCGGGTTCCAGCGTCGTGGTTGACG
TGCTGCGTTACGGCGAGCGTCTGAAAACGCCAGGGCTGAACTTGTTAAGTGCGCCGGGTAACGATGCCGTAGCGACCAGC
GCCCTGGCGGGTGCGGGCTGCCATATGGTGCTGTTCAGTACTGGTCGTGGCACGCCGTATGGTGGATTTGTGCCGACGGT
GAAAATCGCCACCAACAGTGAACTGGCGGCGAAGAAAAAACACTGGATCGACTTTGACGCGGGTCAGTTGATCCACGGTA
AAGCGATGCCGCAGTTGCTGGAAGAATTTATCGACACCATCGTTGAGTTTGCCAACGGTAAGCAAACCTGTAACGAGCGT
AACGACTTCCGCGAACTGGCGATCTTCAAAAGCGGCGTAACGCTATAA

Upstream 100 bases:

>100_bases
CGGCGGACATCAACCTGCTGGGCGAGATGGTGAAAAATATTTGCTTTAACAATGCGCGTGACTACTTCGCCATTGAACTG
AACTAAGGTCTGGGGTTGAT

Downstream 100 bases:

>100_bases
AGTCGAAATGAGTTGTTAATAAAAAACGGCGTTTCATAATGTGAAGCGCCGTTTGCTTTTCCATACCTGCTAAGGATTTA
TCATGACCGCGTATTGGCTG

Product: altronate dehydratase

Products: NA

Alternate protein names: Altronic acid hydratase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKFALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL

Sequences:

>Translated_495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKFALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL
>Mature_495_residues
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKFALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNT
RTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEK
LVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATS
ALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
NDFRELAIFKSGVTL

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789475, Length=495, Percent_Identity=99.3939393939394, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789516, Length=512, Percent_Identity=31.640625, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.7 [H]

Molecular weight: Translated: 54178; Mature: 54178

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKFALTNIAKGANVIKYGL
CCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCEEEEHHHHCCCCEEEECC
PIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGV
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCHHHCCCHHCCCEEEEEEECCCCCCC
RNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLFSHTYGCSQLGDDHINTRTML
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHH
QNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPE
HHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHH
MFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSL
HHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTPY
CCHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCC
GGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
CCCCCEEEEECCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NDFRELAIFKSGVTL
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKFALTNIAKGANVIKYGL
CCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCEEEEHHHHCCCCEEEECC
PIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGV
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCHHHCCCHHCCCEEEEEEECCCCCCC
RNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLFSHTYGCSQLGDDHINTRTML
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHH
QNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIEHLHQ
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LYNVMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPE
HHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHH
MFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSL
HHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GCTQKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTPY
CCHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCC
GGFVPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNER
CCCCCEEEEECCCHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NDFRELAIFKSGVTL
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3038546 [H]