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The map label for this gene is ygiD [H]

Identifier: 157162514

GI number: 157162514

Start: 3226450

End: 3227265

Strand: Reverse

Name: ygiD [H]

Synonym: EcHS_A3216

Alternate gene names: 157162514

Gene position: 3227265-3226450 (Counterclockwise)

Preceding gene: 157162516

Following gene: 157162510

Centisome position: 69.5

GC content: 53.55

Gene sequence:

>816_bases
ATGACACCTTTAGTTAAGGATATCATCATGTCTTCAACACGTATGCCAGCATTGTTTTTAGGTCACGGTAGTCCGATGAA
CGTGCTGGAAGATAATTTGTATACCCGCAGCTGGCAGAAGTTGGGGATGACATTGCCACGCCCGCAAGCGATTGTGGTGG
TTTCGGCTCACTGGTTTACCCGTGGAACAGGAGTGACCGCGATGGAGACGCCGCCCACGATTCATGACTTTGGTGGCTTC
CCGCAGGCGCTGTACGATACGCATTATCCTGCTCCGGGTTCGCCTGCGCTGGCACAGCGTCTGGTTGAGCTGTTAGCGCC
GATCCCGGTGACGCTGGATAAAGAAGCCTGGGGCTTTGACCACGGCTCGTGGGGCGTGCTGATTAAGATGTATCCTGACG
CTGATATCCCGATGGTGCAGTTGAGTATCGACAGTAGCAAACCTGCCGCCTGGCATTTCGAAATGGGGCGCAAACTGGCA
GCGCTGCGAGATGAAGGAATAATGTTGGTCGCCAGCGGTAACGTGGTGCATAACCTGCGCACAGTGAAGTGGCACGGTGA
TAGTTCACCGTATCCGTGGGCGACGTCGTTTAATGAGTATGTGAAAGCGAATCTGACGTGGCAAGGGCCAGTGGAACAAC
ATCCTCTGGTGAATTACCTCGACCATGAAGGTGGCACGTTATCGAACCCAACGCCAGAGCATTATTTGCCGTTGTTGTAT
GTGTTAGGTGCGTGGGATGGGCAGGAGCCAATTACCATTCCGGTTGAGGGTATAGAAATGGGCAGCCTGAGTATGCTGTC
GGTGCAGATAGGCTAA

Upstream 100 bases:

>100_bases
AATCTTCACCTTCAGGCAGATCGTCAAAATAACTGATGATCATCATCATTGTTATCCGGGTTCTTACTCCGCCGAACAAG
CGTATTGTGAGGATATCAAA

Downstream 100 bases:

>100_bases
CAATTACCGGATGGCGAGTGCCATCCGGTATCGTGGCTTATTCAACAAAAATATGTGGATAAAACCGAGACATATCCTGG
GTGATCAATGCACGGTCTTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGF
PQALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLY
VLGAWDGQEPITIPVEGIEMGSLSMLSVQIG

Sequences:

>Translated_271_residues
MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGF
PQALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLY
VLGAWDGQEPITIPVEGIEMGSLSMLSVQIG
>Mature_270_residues
TPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFP
QALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAA
LRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLYV
LGAWDGQEPITIPVEGIEMGSLSMLSVQIG

Specific function: Unknown

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DODA-type extradiol aromatic ring- opening dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI226510974, Length=262, Percent_Identity=100, Blast_Score=536, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014436
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: NA

Molecular weight: Translated: 29904; Mature: 29772

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFT
CCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
RGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFD
CCCCCEEECCCCCHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
HGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIMLVASGNVVHNLR
CCCCEEEEEECCCCCCCEEEEEECCCCCCEEEHHHCCCEEEECCCCEEEEECCCEEEEEE
TVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLY
EEEECCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEE
VLGAWDGQEPITIPVEGIEMGSLSMLSVQIG
EEECCCCCCCEEEEECCCEECCEEEEEEEEC
>Mature Secondary Structure 
TPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFT
CHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
RGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFD
CCCCCEEECCCCCHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCC
HGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIMLVASGNVVHNLR
CCCCEEEEEECCCCCCCEEEEEECCCCCCEEEHHHCCCEEEECCCCEEEEECCCEEEEEE
TVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLY
EEEECCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEE
VLGAWDGQEPITIPVEGIEMGSLSMLSVQIG
EEECCCCCCCEEEEECCCEECCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1314093; 9278503 [H]