Click here to switch to the map view.

The map label for this gene is plsC

Identifier: 157162494

GI number: 157162494

Start: 3207575

End: 3208312

Strand: Reverse

Name: plsC

Synonym: EcHS_A3196

Alternate gene names: 157162494

Gene position: 3208312-3207575 (Counterclockwise)

Preceding gene: 157162495

Following gene: 157162493

Centisome position: 69.09

GC content: 49.46

Gene sequence:

>738_bases
ATGCTATATATCTTTCGTCTTATTATTACCGTGATTTACAGCATCTTAGTCTGTGTATTCGGCTCCATTTACTGCCTTTT
CAGCCCGCGTAACCCGAAACATGTGGCCACCTTTGGGCATATGTTTGGCCGTCTTGCGCCGCTGTTTGGCCTGAAAGTTG
AGTGCCGTAAACCTACAGACGCTGAAAGCTACGGCAATGCTATCTATATCGCTAACCACCAGAACAACTATGACATGGTG
ACAGCATCGAACATCGTGCAACCGCCGACGGTGACGGTAGGTAAAAAGAGCTTGCTGTGGATCCCCTTCTTCGGGCAGTT
GTACTGGTTAACCGGCAACTTATTGATCGACAGAAACAATCGCACTAAAGCTCACGGCACCATTGCGGAAGTAGTGAATC
ACTTCAAAAAACGCCGTATTTCCATCTGGATGTTCCCGGAAGGAACCCGCAGCCGTGGTCGCGGCCTGCTACCGTTCAAG
ACTGGAGCATTTCACGCGGCAATTGCGGCGGGCGTCCCGATTATTCCCGTGTGCGTCTCTACAACTTCGAATAAGATTAA
TCTTAATCGACTGCACAACGGTCTGGTGATTGTCGAAATGCTGCCGCCAATTGACGTCAGTCAGTATGGCAAAGATCAGG
TTCGTGAGCTGGCTGCCCATTGTCGTTCGATAATGGAACAAAAAATCGCCGAGCTCGATAAAGAAGTCGCAGAACGCGAA
GCCGCCGGAAAAGTTTAA

Upstream 100 bases:

>100_bases
CGATTAACAATACGCTTTTCCAGAGAGCGGCTTTTAACAATGCCCTAACCTGATTTCAGGTGACGTACAATGCCAGTCTC
TCAGACCTTCAGAGGGTGTT

Downstream 100 bases:

>100_bases
GTCGGCAATCTGTATTTTTGCGGGGAACACTTTCCTGCACGGTATTACTTTAGCCAGTTTTACATGGAGCAAATATGTCA
CTCAGTCGGCGTCAGTTCAT

Product: 1-acyl-sn-glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: 1-AGP acyltransferase; 1-AGPAT; Lysophosphatidic acid acyltransferase; LPAAT; Phosphatidic acid synthase; PA synthase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRLAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMV
TASNIVQPPTVTVGKKSLLWIPFFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCRSIMEQKIAELDKEVAERE
AAGKV

Sequences:

>Translated_245_residues
MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRLAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMV
TASNIVQPPTVTVGKKSLLWIPFFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCRSIMEQKIAELDKEVAERE
AAGKV
>Mature_245_residues
MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRLAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMV
TASNIVQPPTVTVGKKSLLWIPFFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCRSIMEQKIAELDKEVAERE
AAGKV

Specific function: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the 2 position. This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor

COG id: COG0204

COG function: function code I; 1-acyl-sn-glycerol-3-phosphate acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family

Homologues:

Organism=Homo sapiens, GI5453718, Length=235, Percent_Identity=28.0851063829787, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI15100175, Length=235, Percent_Identity=28.0851063829787, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI6041665, Length=231, Percent_Identity=27.7056277056277, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1789395, Length=245, Percent_Identity=100, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17564032, Length=183, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17568319, Length=214, Percent_Identity=33.6448598130841, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320151, Length=169, Percent_Identity=37.8698224852071, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281364648, Length=236, Percent_Identity=29.2372881355932, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582856, Length=236, Percent_Identity=29.2372881355932, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582854, Length=236, Percent_Identity=29.2372881355932, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24641614, Length=214, Percent_Identity=34.5794392523364, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24641612, Length=214, Percent_Identity=34.5794392523364, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSC_ECOLI (P26647)

Other databases:

- EMBL:   M63491
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S20460
- RefSeq:   AP_003568.1
- RefSeq:   NP_417490.1
- ProteinModelPortal:   P26647
- DIP:   DIP-10516N
- STRING:   P26647
- EnsemblBacteria:   EBESCT00000001573
- EnsemblBacteria:   EBESCT00000014638
- GeneID:   947496
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2986
- KEGG:   eco:b3018
- EchoBASE:   EB1351
- EcoGene:   EG11377
- eggNOG:   COG0204
- GeneTree:   EBGT00050000010343
- HOGENOM:   HBG736534
- OMA:   NEINREA
- ProtClustDB:   PRK15018
- BioCyc:   EcoCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER
- BioCyc:   MetaCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER
- Genevestigator:   P26647
- InterPro:   IPR002123
- InterPro:   IPR004552
- SMART:   SM00563
- TIGRFAMs:   TIGR00530

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.51

Molecular weight: Translated: 27454; Mature: 27454

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRLAPLFGLKVECRKPTD
CHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCC
AESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIPFFGQLYWLTGNLLIDRNN
HHHHCCEEEEEECCCCCCEEEECCCCCCCCEEECCCEEEEEEHHCEEEEEECEEEEECCC
RTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFKTGAFHAAIAAGVPIIPVCVS
CCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
TTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCRSIMEQKIAELDKEVAERE
CCCCEEEHEECCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGKV
HCCCC
>Mature Secondary Structure
MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRLAPLFGLKVECRKPTD
CHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCC
AESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIPFFGQLYWLTGNLLIDRNN
HHHHCCEEEEEECCCCCCEEEECCCCCCCCEEECCCEEEEEEHHCEEEEEECEEEEECCC
RTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFKTGAFHAAIAAGVPIIPVCVS
CCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
TTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCRSIMEQKIAELDKEVAERE
CCCCEEEHEECCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGKV
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1557036; 9278503