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The map label for this gene is guaD [H]

Identifier: 157162344

GI number: 157162344

Start: 3053283

End: 3054599

Strand: Direct

Name: guaD [H]

Synonym: EcHS_A3043

Alternate gene names: 157162344

Gene position: 3053283-3054599 (Clockwise)

Preceding gene: 157162343

Following gene: 157162345

Centisome position: 65.75

GC content: 48.97

Gene sequence:

>1317_bases
ATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGGTCGATAACCCGGAAGAGAT
TGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAA
ACGGAAAGCATCAAATTCCTGACACCATTCGCGTGCGCGACTATCGCGGCAAACTGATAGTACCGGGCTTTGTCGATACA
CATATCCATTATCCGCAAAGTGAAATGGTGGGGGCCTATGGTGAGCAATTGCTGGAGTGGTTGAATAAACACACCTTCCC
TACTGAACGGCGTTATGAGGATTTAGAGTACGCCCGCGAAATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAA
CCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCCGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATG
CGTATGATTGCCGGTAAGGTGATGATGGACCGTAACGCACCGGATTATCTGCTCGACACTGCCGAAAGCAGCTATCACCA
AAGCAAAGAACTGATTGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCAT
CTCCTGAACAGATGGCGATGGCGCAACGCCTGAAAGAAGAATATCCGGATACGTGGGTACATACCCATCTCTGTGAAAAC
AAAGATGAAATTGCCTGGGTGAAATCGCTTTATCCTGACCATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGAC
CGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCA
TTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAA
GTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCA
ATTACAAGACTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACG
ATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTG
CGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCAC
CTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAA

Upstream 100 bases:

>100_bases
TTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAT
TAACAGTAAAGGAGTCAATG

Downstream 100 bases:

>100_bases
TAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAG
GGCGCGCTGGATAATTATTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVK
VGMGTDIGAGTTFNMLQTLNEAYKVLQLQDYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQL
RYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Sequences:

>Translated_438_residues
MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDT
HIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVK
VGMGTDIGAGTTFNMLQTLNEAYKVLQLQDYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQL
RYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN
>Mature_437_residues
SGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTH
IHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMR
MIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCENK
DEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKV
GMGTDIGAGTTFNMLQTLNEAYKVLQLQDYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLR
YDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=448, Percent_Identity=34.8214285714286, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1789249, Length=438, Percent_Identity=99.5433789954338, Blast_Score=915, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319963, Length=418, Percent_Identity=33.4928229665072, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24643849, Length=397, Percent_Identity=33.7531486146096, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50158; Mature: 50026

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIP
CCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCC
DTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYARE
CCEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEECCCCEECCCCCCHHHHC
AESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCC
NLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQDYRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHEEH
LATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGD
EEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHEEECC
DRSIYRTYVDGRLVYERN
CCHHHHEEECCEEEEECC
>Mature Secondary Structure 
SGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQGKVEWFGEWENGKHQIP
CCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCC
DTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLNKHTFPTERRYEDLEYARE
CCEEEEECCCCEEECCEECCCEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINMRMIAGKVMMDRNAPDYLLDT
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEECCCCEECCCCCCHHHHC
AESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQMAMAQRLKEEYPDTWVHTHLCEN
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
KDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTS
CHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCC
NLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQDYRLSAYEAFY
CEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHEEH
LATLGGAKSLGLDDLIGNFLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGD
EEECCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCHHHHHHHHHHEEECC
DRSIYRTYVDGRLVYERN
CCHHHHEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]