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The map label for this gene is hyuA [H]

Identifier: 157162334

GI number: 157162334

Start: 3037542

End: 3038927

Strand: Direct

Name: hyuA [H]

Synonym: EcHS_A3033

Alternate gene names: 157162334

Gene position: 3037542-3038927 (Clockwise)

Preceding gene: 157162333

Following gene: 157162335

Centisome position: 65.41

GC content: 53.17

Gene sequence:

>1386_bases
ATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGATTGAAAGCGGGAT
TGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCCACTGGCTGTTACGTTTTCCCTG
GCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTTACCGGTACCCGC
GCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTGGCCCAAACGGCTGTCGGTTACGCCATCAACT
GGAGGTTTATCGTGGTTATGCCGCCCATAAAGCGGTCATCGATTACAGCTTTCACGGTGTGATCCAGCACATTAATCACG
CAATCCTCGACGAAATCCCGATGATGGCCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTC
AACGATGACGAGGTTTTGCAGGCATTACGCCGTCTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGC
GGCTATCGCCAGCAAGCGGGCGGAGTTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCACGCCTTGAGTCGCCCTCTGG
AATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTG
TCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAACCTGCCCACAATA
TCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCG
AGCAGGACAAACTGTGGTGTGGCATCAGCGATGGTGCGATTGACGTGGTGGCAACCGATCACTGCACCTTCTCGATGGCT
CAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCGCATGCAGTTACT
GTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAACGCTTTGTTGAATTAACCAGCGCAATGCCCGCCAGGCTGT
TTGGCCTGTGGCCGCAAAAAGGATTATTAGCGCCCGGTTCCGACGGCGACGTGGTGATTATCGACCCACGTCAGAGCCAA
CAAATTCAGCATCGCCATCTCCACGACAACGCCGACTACTCGCCATGGGAGGGTTTTACCTGTCAGGGCGCGATTGTCAG
AACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCA
AACCGTTTGTCCCTCCCGTGCTCTAA

Upstream 100 bases:

>100_bases
GCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACCGAATAATTACTCTTCACTTATTCCCCTCCGGTTCGCCG
GAGGGTTTTTGGAGTTTGCT

Downstream 100 bases:

>100_bases
CCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGG
AATGCGCTGGGCGATGACCT

Product: phenylhydantoinase

Products: NA

Alternate protein names: Hydantoin-utilizing enzyme hyuA [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMAEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Sequences:

>Translated_461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMAEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
>Mature_461_residues
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTR
AAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMAEEGLSSFKLYLTYQYKL
NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMA
QRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQ
QIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=466, Percent_Identity=36.480686695279, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=35.0649350649351, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI4503377, Length=462, Percent_Identity=35.2813852813853, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI4503051, Length=462, Percent_Identity=35.2813852813853, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI62422571, Length=462, Percent_Identity=35.2813852813853, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI190194363, Length=462, Percent_Identity=33.982683982684, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI19923821, Length=463, Percent_Identity=34.1252699784017, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI18105007, Length=405, Percent_Identity=23.7037037037037, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI87082175, Length=461, Percent_Identity=99.5661605206074, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1786722, Length=444, Percent_Identity=29.7297297297297, Blast_Score=171, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71989490, Length=467, Percent_Identity=36.4025695931477, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17539558, Length=463, Percent_Identity=36.0691144708423, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI86575075, Length=468, Percent_Identity=27.991452991453, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193204318, Length=409, Percent_Identity=25.4278728606357, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322218, Length=423, Percent_Identity=27.4231678486998, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI221377917, Length=481, Percent_Identity=35.7588357588358, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17137462, Length=468, Percent_Identity=35.6837606837607, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24644287, Length=310, Percent_Identity=36.4516129032258, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24644289, Length=235, Percent_Identity=38.7234042553192, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24642586, Length=408, Percent_Identity=25.9803921568627, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859883, Length=457, Percent_Identity=24.72647702407, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.-.-.- [C]

Molecular weight: Translated: 51016; Mature: 51016

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
CEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCEEEEE
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
EEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHEEE
DYSFHGVIQHINHAILDEIPMMAEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
EHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEE
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
CCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHCCEEE
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
EECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEECCCHH
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
HHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCEE
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
ECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
CEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCEEEEE
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
EEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHEEE
DYSFHGVIQHINHAILDEIPMMAEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
EHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEE
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
CCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHCCEEE
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
EECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEECCCHH
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
HHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCEE
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
ECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA