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The map label for this gene is pphB [H]

Identifier: 157162182

GI number: 157162182

Start: 2877178

End: 2877834

Strand: Direct

Name: pphB [H]

Synonym: EcHS_A2872

Alternate gene names: 157162182

Gene position: 2877178-2877834 (Clockwise)

Preceding gene: 157162181

Following gene: 157162184

Centisome position: 61.96

GC content: 42.16

Gene sequence:

>657_bases
ATGCCATCTACACGCTATCAAAAAATTAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATA
TCAATTATTACAATCCCGCTTACATCAACTCTCTTTTTGCCCTGAAACCGACTTACTTATTTCGGTAGGCGATAACATCG
ATCGTGGGCCGGAGAGTCTTAACGTGCTGCGCCTGCTAAACCAGCCCTGGTTTACGCCGGTTAAAGGCAACCACGAAGCA
ATGGCGCTGGATGCGTTCGCGACCGGCGATGGAAATATGTGGCTTGCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGA
TTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCATTAATGACAACA
TAAAATATGTCATCGCACATGCAGATTATCCGGGTGATGAATATCAGTTTGGTAAAGAAATAGTAGAAAGCGAATTACTC
TGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACA
TATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGCACGCCGAAAAGCGGGCGGCTGTCAT
TTTATAAAATAAAGTAG

Upstream 100 bases:

>100_bases
ATTCCCGATAGTCTTTTGCTATCGGGAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGC
TTATTTTACAGGGTAAAACC

Downstream 100 bases:

>100_bases
TCTCATGCTTCTTCTGTGAAGCATGAGTAACCAAATGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAG
ATCCTGCTTCGCCAGCGCGC

Product: serine/threonine-specific protein phosphatase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTPVKGNHEA
MALDAFATGDGNMWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELL
WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK

Sequences:

>Translated_218_residues
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTPVKGNHEA
MALDAFATGDGNMWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELL
WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
>Mature_217_residues
PSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTPVKGNHEAM
ALDAFATGDGNMWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELLW
PVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK

Specific function: Has been shown, in vitro, to act on Ser, Thr and Tyr- phosphorylated substrates [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1789090, Length=218, Percent_Identity=93.5779816513761, Blast_Score=425, Evalue=1e-121,
Organism=Escherichia coli, GI87081988, Length=217, Percent_Identity=45.6221198156682, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25120; Mature: 24988

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
NVLRLLNQPWFTPVKGNHEAMALDAFATGDGNMWLASGGDWFFDLNDSEQQEAIDLLLKF
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELLWPVDRVQKSLNGELQQINGA
HCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCC
DYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
CEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
>Mature Secondary Structure 
PSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
NVLRLLNQPWFTPVKGNHEAMALDAFATGDGNMWLASGGDWFFDLNDSEQQEAIDLLLKF
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELLWPVDRVQKSLNGELQQINGA
HCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCC
DYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
CEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9278503 [H]