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The map label for this gene is hypD

Identifier: 157162176

GI number: 157162176

Start: 2869554

End: 2870675

Strand: Direct

Name: hypD

Synonym: EcHS_A2866

Alternate gene names: 157162176

Gene position: 2869554-2870675 (Clockwise)

Preceding gene: 157162175

Following gene: 157162177

Centisome position: 61.8

GC content: 55.44

Gene sequence:

>1122_bases
ATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCT
CTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCG
ACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGAC
ACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACAGGG
ATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGG
AGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACCACCGCTATCACTCTGCAACAG
GCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGA
ACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTA
TCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTG
CAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTCGAGTGGTACCGGATGCCGGTAACCTGCTGGCGCA
ACAGGCGATTGCCGATGTGTTCTGTGTCAACGGCGACAGCGAATGGCGCGGCTTAGGCGTGATTGAATCTTCTGGCGTGC
ACCTGACGCCGGATTATCAACGATTCGATGCCGAAGCACATTTCCGCCCGGCACCGCAGCAGGTCTGCGATGACCCGCGC
GCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAAC
CGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGT
GA

Upstream 100 bases:

>100_bases
TAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTG
TTGTATGGCGAGGAAAAATA

Downstream 100 bases:

>100_bases
ATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCC
AACCCGTGGCTGGCAGAGCA

Product: hydrogenase expression/formation protein HypD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRID
TCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLV
QQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPR
ARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA

Sequences:

>Translated_373_residues
MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRID
TCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLV
QQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPR
ARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA
>Mature_373_residues
MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRID
TCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLV
QQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPR
ARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA

Specific function: Required for the formation of all three hydrogenase isoenzymes

COG id: COG0409

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypD family

Homologues:

Organism=Escherichia coli, GI1789084, Length=373, Percent_Identity=100, Blast_Score=776, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPD_ECOLI (P24192)

Other databases:

- EMBL:   X54543
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S15200
- RefSeq:   AP_003296.1
- RefSeq:   NP_417209.1
- ProteinModelPortal:   P24192
- SMR:   P24192
- DIP:   DIP-9998N
- MINT:   MINT-1239502
- STRING:   P24192
- SWISS-2DPAGE:   P24192
- EnsemblBacteria:   EBESCT00000001825
- EnsemblBacteria:   EBESCT00000001826
- EnsemblBacteria:   EBESCT00000001827
- EnsemblBacteria:   EBESCT00000001828
- EnsemblBacteria:   EBESCT00000015301
- GeneID:   947189
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2699
- KEGG:   eco:b2729
- EchoBASE:   EB0481
- EcoGene:   EG10486
- eggNOG:   COG0409
- GeneTree:   EBGT00050000011438
- HOGENOM:   HBG287941
- OMA:   ISGPGCP
- ProtClustDB:   PRK15062
- BioCyc:   EcoCyc:EG10486-MONOMER
- Genevestigator:   P24192
- InterPro:   IPR002780
- PIRSF:   PIRSF005622
- TIGRFAMs:   TIGR00075

Pfam domain/function: PF01924 HypD

EC number: NA

Molecular weight: Translated: 41364; Mature: 41364

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPEN
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCCHHHHHHHCHHHHCCCC
VEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVR
CCEEECCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHCCCCCCCCEEEHHCCCCCEE
IVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITLIP
EEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHH
TLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLV
HHHHHHHCCCCCCCEEECCCEEEEEEECCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
QQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCEECCCHH
RFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGAC
HHCCCCCCCCCCHHHCCCHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCH
AAWYQYRQQESEA
HHHHHHHHHHCCC
>Mature Secondary Structure
MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPEN
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHCCCCHHHHHHHCHHHHCCCC
VEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVR
CCEEECCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHCCCCCCCCEEEHHCCCCCEE
IVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITLIP
EEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHH
TLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLV
HHHHHHHCCCCCCCEEECCCEEEEEEECCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
QQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCEECCCHH
RFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGAC
HHCCCCCCCCCCHHHCCCHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCH
AAWYQYRQQESEA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1849603; 9278503