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The map label for this gene is yfiN [H]

Identifier: 157162080

GI number: 157162080

Start: 2782775

End: 2783998

Strand: Direct

Name: yfiN [H]

Synonym: EcHS_A2763

Alternate gene names: 157162080

Gene position: 2782775-2783998 (Clockwise)

Preceding gene: 157162079

Following gene: 157162081

Centisome position: 59.93

GC content: 48.61

Gene sequence:

>1224_bases
ATGGATAACGATAATTCTCTTAATAAGCGCCCCACGTTTAAAAGAGCATTACGCAACATCAGTATCACCAGCATATTTAT
CACTATGATGCTGATCTGGTTGCTGCTTTCCGTGACCTCGGTGCTGACCCTGAAACAGTACGCGCAAAAAAACCTGGCAC
TGACAGCAGCAACAATGACTTACAGTCTGGAAGCAGCTGTCGTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCA
GCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCGTCCTGGCATTACAC
CCGTAAGGATCCAGGCGATACTTTCAGCAATTTCATAAGCCACTGGCTCTTCCCCGCCCCCATCATTCAGCCGATTCGTC
ACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCC
GTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTGGAAGC
ACTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCG
CTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTACAGGCTAAA
AATGCGCAGCTTCTACGTACCGCGCTACATGACCCATTAACCGGGCTGGCTAACCGCGCAGCGTTTCGTAGCGGCATCAA
CACGTTGATGAACAATTCCGATGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATG
ATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAGTTGGCGGGCTGCGACAT
AAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCAGAATCTGAAGTGCAGCAGATATG
CTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGTCATCAGACCACCATGACATTAAGCATTGGTT
ACGCGATGACCATTGAGCACGCCTCTGCGGAAAAATTACAAGAGCTTGCCGATCACAATATGTATCAGGCCAAACACCAG
CGTGCCGAAAAGCTGGTGAGATAA

Upstream 100 bases:

>100_bases
CACAATAATGACGTCAGATTTGCCGTAAACCTGGATGCCTTATCGCGTAGCGGTGTAAAGGTCAACCCGGATGTCTTAAT
GCTCGCACGGAAGAAAAATG

Downstream 100 bases:

>100_bases
CAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGATTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCC
TCAGGGAAAGTTTACTCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLA
ALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLA
VLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAK
NAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRH
KAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQ
RAEKLVR

Sequences:

>Translated_407_residues
MDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLA
ALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLA
VLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAK
NAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRH
KAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQ
RAEKLVR
>Mature_407_residues
MDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLA
ALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLA
VLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAK
NAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRH
KAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQ
RAEKLVR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=407, Percent_Identity=99.5085995085995, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=31.1258278145695, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=35.5263157894737, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.0512820512821, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=32.0987654320988, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=101, Percent_Identity=41.5841584158416, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45793; Mature: 45793

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMT
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHE
YSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFIS
EEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRHL
HHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHAT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCCC
GDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDL
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
HNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR
CCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMT
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHE
YSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFIS
EEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRHL
HHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHAT
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCCC
GDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDL
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
HNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR
CCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]