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The map label for this gene is hcaD [H]

Identifier: 157162017

GI number: 157162017

Start: 2709604

End: 2710806

Strand: Direct

Name: hcaD [H]

Synonym: EcHS_A2694

Alternate gene names: 157162017

Gene position: 2709604-2710806 (Clockwise)

Preceding gene: 157162016

Following gene: 157162018

Centisome position: 58.35

GC content: 54.03

Gene sequence:

>1203_bases
ATGAAAGAAAAAACGATCATTATTGTCGGTGGCGGGCAAGCGGCGGCAATGGCTGCGGCCTCGCTACGCCAGCAAGGGTT
CACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGG
AAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACC
ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGC
AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATG
CCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCT
GCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCC
CGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCG
ATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGC
GCCAACGAGCAACTGGCTCGCGAGGCCAACCTTGATACTGCCAATGGCATTGTCATTGATGAGGCTTGCCGCACCTGCGA
TCCCGCGATCTTTGCCGGTGGCGATGTGGCAATCACTCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAA
ACGCCAATAACCAGGCGCAAATTGCCGCTGCCGCAATGTTAGGGCTACCGCTACCGCTACTGCCGCCGCCGTGGTTCTGG
AGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGCGATGACTGGCTTTGTCGTGGCAACCCGGAAAC
TCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTCACGCTGAATCAGGGGCGTGAGATTCGCC
CAATTCGCAAATGGATCCAGAGCGGCAAAACGTTTGATGCGAAACTGCTGATAGATGAGAACATCGCGCTTAAATCACTG
TAA

Upstream 100 bases:

>100_bases
TAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCGATTCGCGGCATTCGCCACGTAGCGGCTGGGC
TGGATCTTTAAGGAAGCACG

Downstream 100 bases:

>100_bases
CCAGGATAATTAGCGAATATCTCAATGCCTGGGGCGTGGCGAGGTGCAAGAGTGTGTATTACGTTTAAATCACATTATCT
TGCAAAGGGATTGGTTATGA

Product: phenylpropionate dioxygenase ferredoxin reductase subunit

Products: NA

Alternate protein names: Digoxigenin system ferredoxin--NAD(+) reductase component [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL

Sequences:

>Translated_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL
>Mature_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=393, Percent_Identity=27.735368956743, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI226437568, Length=393, Percent_Identity=27.735368956743, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI65787454, Length=393, Percent_Identity=27.735368956743, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=25.2225519287834, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=25.2225519287834, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=100, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=28.1021897810219, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=23.8390092879257, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=373, Percent_Identity=26.8096514745308, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639257, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281359715, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281359713, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639250, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18543267, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639252, Length=369, Percent_Identity=27.3712737127371, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581020, Length=363, Percent_Identity=25.6198347107438, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573993, Length=363, Percent_Identity=25.8953168044077, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585130, Length=326, Percent_Identity=23.0061349693252, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 43979; Mature: 43979

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCHHHH
QVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
HHCCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELAASATQRRCKVTVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCEEEEHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEECCC
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
CCHHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHCCCCCCCCCCHH
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL
EEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCEEECCC
>Mature Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCHHHH
QVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
HHCCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELAASATQRRCKVTVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCEEEEHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEECCC
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
CCHHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHCCCCCCCCCCHH
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL
EEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA