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The map label for this gene is gcvR [H]

Identifier: 157161941

GI number: 157161941

Start: 2621747

End: 2622385

Strand: Direct

Name: gcvR [H]

Synonym: EcHS_A2611

Alternate gene names: 157161941

Gene position: 2621747-2622385 (Clockwise)

Preceding gene: 157161933

Following gene: 157161942

Centisome position: 56.46

GC content: 48.36

Gene sequence:

>639_bases
TTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATC
GCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTT
GCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGG
AATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGAC
GGCGCGTCCGCGTCCGCCAATGCCAGCATCTGTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCT
TCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCT
GCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCAAGCGTTCAAAGCCCT
ATGTACAGAACTCAATGCACAAGGCAGTATTAACGTCGTCAATTATTCCCAACATGATGAACAGGATGGAGTTAAGTAA

Upstream 100 bases:

>100_bases
GACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCA
AGCAGACAGAACCGTTCTGA

Downstream 100 bases:

>100_bases
TGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGAC
TTCCAGGGACAGCGTGTTCT

Product: glycine cleavage system transcriptional repressor

Products: NA

Alternate protein names: Gcv operon repressor [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLYACFFYAFLKNNSPLKGITSLTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSW
NAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERA
AQLHIQITAHSPASADAANIEQAFKALCTELNAQGSINVVNYSQHDEQDGVK

Sequences:

>Translated_212_residues
MLYACFFYAFLKNNSPLKGITSLTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSW
NAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERA
AQLHIQITAHSPASADAANIEQAFKALCTELNAQGSINVVNYSQHDEQDGVK
>Mature_212_residues
MLYACFFYAFLKNNSPLKGITSLTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSW
NAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERA
AQLHIQITAHSPASADAANIEQAFKALCTELNAQGSINVVNYSQHDEQDGVK

Specific function: Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how gcvR acts as a repressor. It does not seem to bind DNA. It could interact with gcvA and suppress its activato

COG id: COG2716

COG function: function code E; Glycine cleavage system regulatory protein

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082113, Length=190, Percent_Identity=99.4736842105263, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR016867 [H]

Pfam domain/function: PF01842 ACT [H]

EC number: NA

Molecular weight: Translated: 23262; Mature: 23262

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYACFFYAFLKNNSPLKGITSLTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIED
CCHHHEEHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
SRLAMLGEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWV
CHHEECCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
QVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANI
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHH
EQAFKALCTELNAQGSINVVNYSQHDEQDGVK
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLYACFFYAFLKNNSPLKGITSLTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIED
CCHHHEEHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
SRLAMLGEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWV
CHHEECCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
QVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANI
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHH
EQAFKALCTELNAQGSINVVNYSQHDEQDGVK
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537378; 9205837; 9278503; 2016588 [H]