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The map label for this gene is gatD [H]

Identifier: 157161575

GI number: 157161575

Start: 2214184

End: 2215224

Strand: Reverse

Name: gatD [H]

Synonym: EcHS_A2227

Alternate gene names: 157161575

Gene position: 2215224-2214184 (Counterclockwise)

Preceding gene: 157161576

Following gene: 157161574

Centisome position: 47.71

GC content: 48.7

Gene sequence:

>1041_bases
ATGAAATCAGTGGTGAATGATACTGATGGTATCGTGCGCGTTGCAGAAAGCGTCATTCCTGAAATTAAACATCAGGATGA
GGTGCGGGTAAAAATTGCCAGCTCGGGCTTATGTGGTTCCGATTTACCCAGGATATTTAAAAATGGTGCACATTATTATC
CAATAACGTTAGGCCATGAATTTAGCGGCTATATTGATGCGGTGGGATCCGGTGTTGATGATTTACACCCTGGCGATGCG
GTTGCCTGTGTGCCGTTATTACCCTGTTTTACTTGTCCAGAGTGTCTGAAAGGGTTTTATTCCCAGTGCGCAAAATATGA
TTTTATTGGCTCGCGGCGTGATGGTGGATTTGCTGAATATATTGTCGTTAAGCGAAAAAATGTCTTTGCTCTACCCACGG
ATATGCCTATTGAGGATGGGGTTTTTATTGAGCCGATTACCGTTGGTCTGCATGCTTTTCATTTAGCGCAAGGTTGTGAG
AATAAAAACGTTATTATTATTGGTGCCGGAACCATTGGCCTGCTGGCCATTCAGTGCGCTGTCGCGCTGGGAGCAAAGAG
TGTGACGGCGATCGACATTAGTTCAGAAAAACTGGCACTGGCAAAATCTTTCGGTGCGATGCAAACATTTAACAGTAGCG
AAATGAGCGCGCCGCAAATGCAGAGCGTTTTACGCGAACTGCGCTTTAATCAGCTTATCCTCGAGACGGCTGGCGTACCG
CAAACTGTCGAACTGGCGGTAGAGATTGCCGGTCCTCATGCCCAACTGGCGCTGGTGGGCACGTTGCATCAGGATCTGCA
TTTAACATCGGCAACGTTTGGCAAAATATTGCGTAAAGAGCTGACGGTTATCGGCAGTTGGATGAACTACTCCAGCCCTT
GGCCGGGGCAGGAGTGGGAAACGGCGAGCCGGTTGCTGACAGAACGTAAGTTAAGCCTGGAGCCATTAATCGCTCACCGT
GGAAGCTTTGAAAGCTTCGCCCAGGCGGTGCGTGACATCGCTCGTAATGCTATGCCGGGCAAAGTGTTGCTCATTCCCTG
A

Upstream 100 bases:

>100_bases
GGCGTAGAGCGCGTGGCTTTATTAAACAAGAGAAAGTCGTTCTCGCAGAATAATTTTTACCTGAGGGGGAGGTTATCCCC
CTTAACAATCAGGAGTTTTT

Downstream 100 bases:

>100_bases
AACCGCGGGCCAGCGTGATGCTGGCCCGGTATTGTGCAAAACAGATCATTCACCAATGGTCCCCCTTCGTTTACACTAGC
CACAATTGAATATGGGTAAA

Product: galactitol-1-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGVFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP

Sequences:

>Translated_346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGVFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP
>Mature_346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGVFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=351, Percent_Identity=27.0655270655271, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4501929, Length=224, Percent_Identity=29.4642857142857, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI4501939, Length=338, Percent_Identity=24.8520710059172, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI156523966, Length=338, Percent_Identity=24.8520710059172, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI34577061, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4501933, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI71565154, Length=338, Percent_Identity=24.8520710059172, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI71743840, Length=228, Percent_Identity=25.8771929824561, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI262073058, Length=228, Percent_Identity=25.8771929824561, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788407, Length=346, Percent_Identity=99.7109826589595, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=29.2537313432836, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787863, Length=333, Percent_Identity=31.8318318318318, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI226510992, Length=318, Percent_Identity=29.559748427673, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790045, Length=344, Percent_Identity=26.453488372093, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790718, Length=336, Percent_Identity=28.2738095238095, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI87082125, Length=337, Percent_Identity=28.1899109792285, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788075, Length=312, Percent_Identity=25, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786825, Length=317, Percent_Identity=25.8675078864353, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786518, Length=272, Percent_Identity=28.3088235294118, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=325, Percent_Identity=29.2307692307692, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17562878, Length=229, Percent_Identity=31.8777292576419, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25146526, Length=341, Percent_Identity=24.9266862170088, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=350, Percent_Identity=24, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=323, Percent_Identity=25.077399380805, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323099, Length=354, Percent_Identity=28.8135593220339, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322619, Length=338, Percent_Identity=30.4733727810651, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319955, Length=338, Percent_Identity=30.4733727810651, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319258, Length=236, Percent_Identity=29.2372881355932, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319257, Length=236, Percent_Identity=28.3898305084746, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=353, Percent_Identity=23.5127478753541, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=290, Percent_Identity=26.551724137931, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17137530, Length=204, Percent_Identity=33.8235294117647, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17737897, Length=204, Percent_Identity=34.3137254901961, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17737895, Length=232, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221457811, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550770, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551930, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.251 [H]

Molecular weight: Translated: 37419; Mature: 37419

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCC
FSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEY
CHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVVKRKNVFALPTDMPIEDGVFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCA
EEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
HHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWE
CEEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TASRLLTERKLSLEPLIAHRGSFESFAQAVRDIARNAMPGKVLLIP
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCC
FSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEY
CHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVVKRKNVFALPTDMPIEDGVFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCA
EEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
HHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWE
CEEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TASRLLTERKLSLEPLIAHRGSFESFAQAVRDIARNAMPGKVLLIP
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]