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The map label for this gene is rfbB1 [H]

Identifier: 157161536

GI number: 157161536

Start: 2160574

End: 2161638

Strand: Reverse

Name: rfbB1 [H]

Synonym: EcHS_A2182

Alternate gene names: 157161536

Gene position: 2161638-2160574 (Counterclockwise)

Preceding gene: 157161537

Following gene: 157161535

Centisome position: 46.55

GC content: 58.87

Gene sequence:

>1065_bases
ATGAAGATTCTTGTCACCGGTGGTGCAGGCTTTATCGGCTCTGCTGTAGTTCGTCATATCATTGAAAATACCCGGGATGA
AGTCCGCGTGATGGACTGCCTGACCTATGCCGGCAACCTCGAATCCCTGGCGCCGGTGGCCGGGAGCGAACGCTACTCGT
TTTCCCAGACCGATATCACCGATGCCGCTGCCGTGGCGGCCCAGTTCAGCGAGTTCCGCCCGGATATCGTGATGCATCTG
GCGGCAGAAAGTCATGTGGACCGTTCCATTGATGGCCCGGCCGCCTTCATCCAGACCAACGTGATCGGCACCTTCACTCT
GCTGGAGGCGGCCCGTCACTACTGGTCCGGGCTTGGGGACGCGCAGAAGCAGGCCTTCCGCTTCCACCATATTTCCACCG
ATGAGGTGTACGGCGACCTGCACGGCACCGATGACCTGTTCACCGAAGAGACTCCGTACGCCCCGAGCAGCCCGTACTCT
GCCTCCAAAGCGGGCAGCGACCATCTGGTTCGCGCCTGGAACCGCACCTACGGCCTGCCGGTGGTGGTGACCAACTGCTC
CAACAACTATGGTCCGTATCACTTCCCGGAGAAACTGATCCCGCTGACTATCCTTAATGCCCTGGCGGGTAAACCCCTGC
CGGTGTATGGCAACGGGGAGCAGATCCGTGACTGGCTGTATGTTGAGGACCATGCCCGTGCGCTGTATAAAGTGGCGACC
GAAGGCAAGAGCGGCGAAACCTACAATATTGGCGGTCATAACGAGCGTAAAAATATCGATGTGGTGCGCACCATCTGCGC
CATTCTCGACAAGGTGGTGGCGCAGAAGCCGGGCAACATCGCCCACTTCGCTGACCTGATCACTTTTGTCACCGACCGTC
CGGGACACGACCTGCGTTATGCCATTGATGCCGCGAAAATTCAGCGCGATCTGGGCTGGGTGCCGCAGGAGACGTTCGAG
AGCGGGATTGAAAAAACCGTGCACTGGTATCTTAACAACCAGACCTGGTGGCAGCGCGTGCTGGATGGCTCCTATGCCGG
TGAGCGTCTGGGCCTAAATAACTGA

Upstream 100 bases:

>100_bases
CCTTCGATATTATCCCCATAAAATTACATCTCAAACATTTTAATATGGAGATGTATGCAATTATTTTCTTTTTGCATGAA
TAATCAAAGGTAAATAAAAT

Downstream 100 bases:

>100_bases
TATCTGACTGATTAAGGAATAGATTTATGAAAGGTATTGTTCTGGCCGGCGGCTCCGGCACGCGTTTATATCCGATCACC
CAGGGCGTCTCCAAGCAGCT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKILVTGGAGFIGSAVVRHIIENTRDEVRVMDCLTYAGNLESLAPVAGSERYSFSQTDITDAAAVAAQFSEFRPDIVMHL
AAESHVDRSIDGPAAFIQTNVIGTFTLLEAARHYWSGLGDAQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYS
ASKAGSDHLVRAWNRTYGLPVVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQIRDWLYVEDHARALYKVAT
EGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIAHFADLITFVTDRPGHDLRYAIDAAKIQRDLGWVPQETFE
SGIEKTVHWYLNNQTWWQRVLDGSYAGERLGLNN

Sequences:

>Translated_354_residues
MKILVTGGAGFIGSAVVRHIIENTRDEVRVMDCLTYAGNLESLAPVAGSERYSFSQTDITDAAAVAAQFSEFRPDIVMHL
AAESHVDRSIDGPAAFIQTNVIGTFTLLEAARHYWSGLGDAQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYS
ASKAGSDHLVRAWNRTYGLPVVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQIRDWLYVEDHARALYKVAT
EGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIAHFADLITFVTDRPGHDLRYAIDAAKIQRDLGWVPQETFE
SGIEKTVHWYLNNQTWWQRVLDGSYAGERLGLNN
>Mature_354_residues
MKILVTGGAGFIGSAVVRHIIENTRDEVRVMDCLTYAGNLESLAPVAGSERYSFSQTDITDAAAVAAQFSEFRPDIVMHL
AAESHVDRSIDGPAAFIQTNVIGTFTLLEAARHYWSGLGDAQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYS
ASKAGSDHLVRAWNRTYGLPVVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQIRDWLYVEDHARALYKVAT
EGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIAHFADLITFVTDRPGHDLRYAIDAAKIQRDLGWVPQETFE
SGIEKTVHWYLNNQTWWQRVLDGSYAGERLGLNN

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=34.4117647058824, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=27.2455089820359, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=24.3553008595989, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=76.1363636363636, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=70.8215297450425, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1786974, Length=357, Percent_Identity=28.2913165266106, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788366, Length=358, Percent_Identity=24.3016759776536, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=35.9882005899705, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=34.2424242424242, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=26.0355029585799, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=376, Percent_Identity=25, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=374, Percent_Identity=25.668449197861, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901790, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=195, Percent_Identity=26.1538461538462, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=196, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=382, Percent_Identity=25.9162303664921, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=348, Percent_Identity=26.4367816091954, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=27.6243093922652, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24158427, Length=284, Percent_Identity=24.6478873239437, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39254; Mature: 39254

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIIENTRDEVRVMDCLTYAGNLESLAPVAGSERYSFSQTDIT
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHH
DAAAVAAQFSEFRPDIVMHLAAESHVDRSIDGPAAFIQTNVIGTFTLLEAARHYWSGLGD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYSASKAGSDHLVRAWNRTYGLP
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
VVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQIRDWLYVEDHARALYKVAT
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHC
EGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIAHFADLITFVTDRPGHDLRY
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
AIDAAKIQRDLGWVPQETFESGIEKTVHWYLNNQTWWQRVLDGSYAGERLGLNN
HHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIIENTRDEVRVMDCLTYAGNLESLAPVAGSERYSFSQTDIT
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHH
DAAAVAAQFSEFRPDIVMHLAAESHVDRSIDGPAAFIQTNVIGTFTLLEAARHYWSGLGD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQKQAFRFHHISTDEVYGDLHGTDDLFTEETPYAPSSPYSASKAGSDHLVRAWNRTYGLP
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
VVVTNCSNNYGPYHFPEKLIPLTILNALAGKPLPVYGNGEQIRDWLYVEDHARALYKVAT
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHC
EGKSGETYNIGGHNERKNIDVVRTICAILDKVVAQKPGNIAHFADLITFVTDRPGHDLRY
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
AIDAAKIQRDLGWVPQETFESGIEKTVHWYLNNQTWWQRVLDGSYAGERLGLNN
HHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]