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The map label for this gene is insQ [H]

Identifier: 157161403

GI number: 157161403

Start: 2029204

End: 2029533

Strand: Reverse

Name: insQ [H]

Synonym: EcHS_A2033

Alternate gene names: 157161403

Gene position: 2029533-2029204 (Counterclockwise)

Preceding gene: 157161405

Following gene: 157161399

Centisome position: 43.71

GC content: 48.79

Gene sequence:

>330_bases
ATGAAACGACTACAGGCATTCAAATTCCGGTTAAGACCAGGTGGTCAACAGGAGCGTGAAATGAGGCGCTTCGCAGGCGC
ATGTCGTTTCGTTTTCAATCGTGCTCTGGCGCTTCAGAATGAGAATCATGAGGCCGGTAATAAATACATCCCTTACGGGA
AAATGGCTTCCTGGTTGGTTGAGTGGAAAAATGCCACTGAAACGCAATGGCTTAAAGATTCACCCTCACAGCCATTGCAA
CAGTCACTGAAAGACCCTGAGCGGGCTTACAAAAACTTCTTCCGGCTTCGTCACCACGCTCAAACGGTGTGCTACCTATC
GCGGCTATAA

Upstream 100 bases:

>100_bases
TTTAGTAACGAAGACTAGGTGAACATGCTTCAGGAAAACACAATGCCTTCCGCGTCGGATATCGGTTTCTTTTTTCATAG
GCCAAAGTATGATTTGGCCC

Downstream 100 bases:

>100_bases
AAACAGCTTGTCCATCAGCACGATAGATAAAATAAGGCTGCTTTTTGTCGCCTTCTTTTTTCCACTCAAACCAGCCATCG
GCAAAACAGATTGCCCGACC

Product: IS605 family transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDPERAYKNFFRLRHHAQTVCYLSRL

Sequences:

>Translated_109_residues
MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDPERAYKNFFRLRHHAQTVCYLSRL
>Mature_109_residues
MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDPERAYKNFFRLRHHAQTVCYLSRL

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=77, Percent_Identity=96.1038961038961, Blast_Score=158, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 13018; Mature: 13018

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLV
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQTVCYLSRL
HHCCCCCCHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLV
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQTVCYLSRL
HHCCCCCCHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]