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The map label for this gene is motA

Identifier: 157161362

GI number: 157161362

Start: 1995423

End: 1996310

Strand: Reverse

Name: motA

Synonym: EcHS_A1986

Alternate gene names: 157161362

Gene position: 1996310-1995423 (Counterclockwise)

Preceding gene: 157161363

Following gene: 157161361

Centisome position: 42.99

GC content: 51.46

Gene sequence:

>888_bases
GTGCTTATCTTATTAGGTTACCTGGTTGTTCTCGGTACAGTTTTCGGCGGTTATTTGATGACCGGTGGAAGCCTTGGAGC
ACTCTATCAACCCGCTGAACTGGTGATTATTGCCGGTGCAGGGATTGGGTCGTTTATCGTCGGCAATAATGGCAAAGCGA
TTAAAGGCACGCTGAAGGCGCTGCCGTTGCTGTTTCGTCGCTCCAAATACACCAAAGCAATGTATATGGATCTGCTGGCT
CTGCTTTATCGGTTGATGGCGAAATCGCGGCAGATGGGGATGTTTTCGCTGGAACGTGATATTGAAAATCCCCGTGAGAG
CGAGATCTTCGCCAGCTACCCACGCATCCTCGCGGATAGCGTCATGCTTGATTTTATCGTCGATTATCTGCGCCTGATTA
TCAGCGGTCACATGAACACCTTCGAAATCGAAGCCCTGATGGATGAAGAGATTGAGACGCACGAAAGCGAGGCAGAAGTC
CCGGCGAACAGTCTGGCGCTGGTCGGGGACTCACTTCCGGCGTTTGGTATTGTTGCGGCTGTAATGGGGGTCGTTCACGC
GTTAGGTTCAGCCGATCGTCCTGCCGCCGAGCTGGGTGCGCTTATCGCACATGCGATGGTGGGGACTTTCCTCGGCATTT
TATTGGCTTACGGATTTATTTCCCCATTAGCGACTGTTTTACGTCAGAAAAGCGCCGAAACCAGCAAAATGATGCAGTGC
GTCAAAGTCACTCTGCTTTCTAATCTGAACGGTTACGCACCGCCTATCGCCGTTGAGTTTGGTCGCAAAACGCTCTATTC
CAGCGAACGTCCGTCGTTTATTGAACTGGAAGAGCATGTGCGTGCGGTGAAAAATCCGCAACAACAGACGACAACCGAGG
AAGCATGA

Upstream 100 bases:

>100_bases
GCAGCGGTAACGACGTACCGCTGCTTTTTTTTGCCCCAATCGCGCGTTAACGCCTGACGACTGAACATCCTGTCATGGTC
AACAGTGGAAGGATGATGTC

Downstream 100 bases:

>100_bases
AGAATCAAGCGCATCCGATTATTGTCGTCAAACGACGCAAAGCCAAAAGCCACGGGGCAGCACATGGATCGTGGAAGATT
GCTTATGCCGACTTTATGAC

Product: flagellar motor protein MotA

Products: NA

Alternate protein names: Chemotaxis protein MotA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGSFIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLA
LLYRLMAKSRQMGMFSLERDIENPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQC
VKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA

Sequences:

>Translated_295_residues
MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGSFIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLA
LLYRLMAKSRQMGMFSLERDIENPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQC
VKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA
>Mature_295_residues
MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGSFIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLA
LLYRLMAKSRQMGMFSLERDIENPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQC
VKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA

Specific function: MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a yhjH disruption, (a c-di-GM

COG id: COG1291

COG function: function code N; Flagellar motor component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MotA family

Homologues:

Organism=Escherichia coli, GI1788199, Length=295, Percent_Identity=100, Blast_Score=594, Evalue=1e-171,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): MOTA_ECOLI (P09348)

Other databases:

- EMBL:   J01652
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A30279
- RefSeq:   AP_002510.1
- RefSeq:   NP_416404.1
- ProteinModelPortal:   P09348
- DIP:   DIP-10244N
- MINT:   MINT-1250843
- STRING:   P09348
- EnsemblBacteria:   EBESCT00000000793
- EnsemblBacteria:   EBESCT00000017945
- GeneID:   947564
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1879
- KEGG:   eco:b1890
- EchoBASE:   EB0596
- EcoGene:   EG10601
- eggNOG:   COG1291
- GeneTree:   EBGT00050000008322
- HOGENOM:   HBG299720
- OMA:   MAIEGDI
- ProtClustDB:   PRK09110
- BioCyc:   EcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN
- Genevestigator:   P09348
- GO:   GO:0006935
- InterPro:   IPR000540
- InterPro:   IPR022522
- InterPro:   IPR002898
- TIGRFAMs:   TIGR03818

Pfam domain/function: PF01618 MotA_ExbB

EC number: NA

Molecular weight: Translated: 32012; Mature: 32012

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01307 MOTA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x156f8f88)-; HASH(0x153ccd14)-; HASH(0x1504e5c4)-; HASH(0x155b70c4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGSFIVGNNGKAIKGTLKA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHH
LPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIENPRESEIFASYPRILADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEVPANSLALVGDSLPAFGIVAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHH
VMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCHHHCCCCCH
>Mature Secondary Structure
MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGSFIVGNNGKAIKGTLKA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHH
LPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIENPRESEIFASYPRILADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEVPANSLALVGDSLPAFGIVAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHH
VMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYGFISPLATVLRQKSAETSKMMQC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6090403; 9097040; 9278503; 3007435; 1719217; 7643400