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The map label for this gene is znuB

Identifier: 157161328

GI number: 157161328

Start: 1962391

End: 1963176

Strand: Direct

Name: znuB

Synonym: EcHS_A1952

Alternate gene names: 157161328

Gene position: 1962391-1963176 (Clockwise)

Preceding gene: 157161327

Following gene: 157161331

Centisome position: 42.26

GC content: 51.91

Gene sequence:

>786_bases
ATGATTGAATTATTATTTCCCGGTTGGTTAGCCGGGATCATGCTCGCCTGTGCCGCGGGTCCGCTGGGTTCGTTTGTAGT
CTGGCGTCGTATGTCTTATTTCGGTGATACGCTGGCTCATGCCTCATTACTTGGCGTCGCGTTTGGTTTGTTGCTGGACG
TGAATCCATTCTATGCGGTGATTGCCGTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTG
GCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATAT
TCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGTGACGCCAGAAGATCTCATCTCTATTGCGATTGGCG
TGGTCATCGTGGTGGCTATTTTGTTCTGGCAATGGCGCAATTTGCTGTCAATGACGATAAGCCCGGATCTGGCGTTTGTT
GATGGTGTGAAATTACAGCGAGTGAAATTGTTGTTGATGCTGGTGACGGCATTGACGATTGGTGTAGCGATGAAATTTGT
CGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGG
CTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGT
CCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAA

Upstream 100 bases:

>100_bases
GTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTG
CGTCGGGGAAATGATCGCTC

Downstream 100 bases:

>100_bases
TCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGCTATCTCTGGCTGGCCTGATA
AGACGCGAACAGCGTCGCAT

Product: high-affinity zinc transporter membrane component

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQL
AIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAG
PSVVLCAALLFILSMMKKQAS

Sequences:

>Translated_261_residues
MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQL
AIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAG
PSVVLCAALLFILSMMKKQAS
>Mature_261_residues
MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQL
AIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAG
PSVVLCAALLFILSMMKKQAS

Specific function: Involved in the high-affinity zinc uptake transport system

COG id: COG1108

COG function: function code P; ABC-type Mn2+/Zn2+ transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC-3 integral membrane protein family

Homologues:

Organism=Escherichia coli, GI1788166, Length=261, Percent_Identity=100, Blast_Score=506, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZNUB_ECOLI (P39832)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U38702
- EMBL:   M21298
- PIR:   C64948
- RefSeq:   AP_002479.1
- RefSeq:   NP_416373.1
- ProteinModelPortal:   P39832
- STRING:   P39832
- EnsemblBacteria:   EBESCT00000003079
- EnsemblBacteria:   EBESCT00000017313
- GeneID:   946372
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1848
- KEGG:   eco:b1859
- EchoBASE:   EB2271
- EcoGene:   EG12368
- eggNOG:   COG1108
- GeneTree:   EBGT00050000009961
- HOGENOM:   HBG733179
- OMA:   FMHEVRI
- ProtClustDB:   PRK09543
- BioCyc:   EcoCyc:ZNUB-MONOMER
- Genevestigator:   P39832
- InterPro:   IPR001626

Pfam domain/function: PF00950 ABC-3

EC number: NA

Molecular weight: Translated: 27729; Mature: 27729

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14dbdb24)-; HASH(0x14d131d8)-; HASH(0x1561ad88)-; HASH(0x147e1eb4)-; HASH(0x15636f50)-; HASH(0x11a9a7e0)-; HASH(0x14d1103c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAV
CCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLL
HHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFVDGVKLQRVKLLLMLVTALTI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHH
GVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PSVVLCAALLFILSMMKKQAS
HHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAV
CCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLL
HHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFVDGVKLQRVKLLLMLVTALTI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHH
GVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PSVVLCAALLFILSMMKKQAS
HHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 2842314; 7984428; 9680209