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The map label for this gene is prtB [H]

Identifier: 157161312

GI number: 157161312

Start: 1945756

End: 1947816

Strand: Reverse

Name: prtB [H]

Synonym: EcHS_A1936

Alternate gene names: 157161312

Gene position: 1947816-1945756 (Counterclockwise)

Preceding gene: 157161315

Following gene: 157161308

Centisome position: 41.95

GC content: 49.83

Gene sequence:

>2061_bases
ATGCTACCAAAAGCCGCCCGCATTCCCCACGCCATGACGCTTCATGGCGATACGCGCATCGATAATTACTACTGGCTGCG
GGACGATACGCGTTCTCAGCCGGAAGTCCTGGACTACCTGCAACAAGAAAATAGTTACGGTCATCGGGTGATGGCCTCAC
AACAAGCCTTGCAGGATCGCATCTTAAAGGAAATCATCGACCGCATTCCGCAAAGAGAAGTTTCTGCGCCCTACATCAAA
AATGGCTACCGCTATCGGCATATTTATGAACCAGGCTGTGAATATGCTATCTACCAGCGTCAATCGGCGTTCAGTGAAGA
GTGGGACGAGTGGGAAATATTGCTCGATGCCAACAAGCGCGCGGCTCATAGTGAGTTTTATTCGATGGGCGGAATGGCGA
TTACGCCCGATAACACCATTATGGCGCTGGCAGAAGATTTTCTTTCCCGACGCCAGTACGGCATTCGTTTTCGTAATCTG
GAAACAGGTAACTGGTACCCGGAACTGCTGGATAACGTTGAACCCAGCTTTGTCTGGGCAAATGACTCCTGGACTTTCTA
CTATGTTCGCAAGCATCCAGTGACGCTGCTGCCTTATCAGGTCTGGCGTCACGCTATCGGTACTCCAGCATCGCAAGATA
AACTGATCTACGAAGAAAAAGACGATACCTATTACGTCAGCCTGCATAAAACGACGTCGAAGCACTATGTAGTCATTCAT
TTGGCCAGCGCCACCACCAGTGAAGTTCGCCTGCTGGACGCGGAAATGGCCGATGCCGAGCCGTTTGTTTTTCTGCCGCG
CCGCAAAGATCACGAATACAGCCTTGATCACTACCAGCATCGGTTTTATCTGCGTTCCAACCGCAACGGCAAAAACTTTG
GCTTATACCGTACCCGTATGCGTGATGAGCAACAGTGGGAAGAGTTAATTCCGCCACGCGAAAACATCATGCTGGAAGGG
TTTACGCTGTTTACCGACTGGCTGGTGGTTGAAGAGCGTCAGCGCGGGTTAACCAGTTTGCGCCAAATTAACCGCAAGAC
CCGGGAAGTCATTGGTATTGCCTTTGATGATCCGGCCTATGTGACCTGGATTGCCTACAATCCAGAATCTGAAACCGCGC
GATTGCGTTATGGTTATTCTTCCATGACCACACCAGACACTTTGTTTGAACTGGATATGGATACCGGTGAGCGTCGTGTA
TTAAAACAAACGGAAGTTCCTGGTTTTGATGCGGCGAATTACCGCAGTGAACACCTGTGGATAGTCGCCCGTGATGGCGT
CGAAGTTCCGGTTTCGCTGGTCTATCATCGCAAACATTTTCGCAAAGGACACAACCCGCTGCTGGTGTATGGCTATGGTT
CTTACGGCGCAAGTATTGATGCCGATTTCAGTTTTAGCCGCTTGAGTTTGTTAGATCGTGGCTTTGTCTACGCCATTGTC
CATGTTCGCGGCGGTGGTGAGCTGGGGCAACAATGGTACGAAGACGGAAAATTTCTGAAGAAGAAAAATACGTTTAATGA
TTATCTTGATGCCTGCGATGCATTGTTAAAACTGGGCTATGGCTCTCCTTCGCTTTGTTATGCGATGGGCGGGAGTGCGG
GGGGCATGTTGATGGGCGTTGCGATTAATGCACGCCCTGAATTATTCCACGGCGTTATCGCCCAGGTACCGTTTGTTGAT
GTTGTAACAACAATGCTTGATGAATCAATTCCTCTTACCACTGGTGAGTTTGAAGAGTGGGGGAATCCGCAGGATCCGCA
ATATTACGAGTATATGAAAAGCTACAGCCCATATGACAACGTCACCGCACAGGCTTATCCGCATTTACTGGTAACGACCG
GTTTGCACGATTCTCAGGTGCAATATTGGGAACCGGCAAAATGGGTCGCTAAATTGCGCGAGCTGAAAACCGATGACCAT
CTTTTATTGCTCTGTACCGACATGGACTCAGGCCATGGCGGTAAATCTGGTCGCTTTAAATCGTACGAAGGCGTAGCGAT
GGAATATGCTTTTCTGGTCGCGCTGGCGCAGGGAACATTACCCGCTACGCCTGCGGATTAA

Upstream 100 bases:

>100_bases
ATTACCTGTCATCATCTAAGCAATGACTGCCCTGTTTCGCTTGCATCCCCGGTGAGTTTTGCCACCCTTATAAGATGTTT
CAACCAGAAAGAACAATAAC

Downstream 100 bases:

>100_bases
GTATTTTCCAGATAATGTTTCAGTGTTAAACGCAGCTCCGGGCTCATGCTGTCCAGGTTATTAAATAACCAGTGCAGATA
GCCCGGATCGCGTTCGGCAA

Product: protease 2

Products: NA

Alternate protein names: Oligopeptidase B; Protease II [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINARPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPATPAD

Sequences:

>Translated_686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINARPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPATPAD
>Mature_686_residues
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIK
NGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEG
FTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELDMDTGERRV
LKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINARPELFHGVIAQVPFVD
VVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDH
LLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPATPAD

Specific function: Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [H]

COG id: COG1770

COG function: function code E; Protease II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=696, Percent_Identity=24.8563218390805, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI284172420, Length=474, Percent_Identity=30.168776371308, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI284172413, Length=474, Percent_Identity=30.168776371308, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI70778815, Length=474, Percent_Identity=30.168776371308, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI284172438, Length=474, Percent_Identity=30.168776371308, Blast_Score=180, Evalue=5e-45,
Organism=Homo sapiens, GI284172431, Length=474, Percent_Identity=30.168776371308, Blast_Score=180, Evalue=5e-45,
Organism=Homo sapiens, GI108860686, Length=214, Percent_Identity=40.6542056074766, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI108860692, Length=213, Percent_Identity=40.8450704225352, Blast_Score=149, Evalue=9e-36,
Organism=Escherichia coli, GI1788150, Length=686, Percent_Identity=99.1253644314869, Blast_Score=1417, Evalue=0.0,
Organism=Drosophila melanogaster, GI24583414, Length=701, Percent_Identity=23.2524964336662, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221510989, Length=661, Percent_Identity=25.5673222390318, Blast_Score=155, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002471
- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: =3.4.21.83 [H]

Molecular weight: Translated: 79353; Mature: 79353

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00708 PRO_ENDOPEP_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDR
CCCCCCCCCEEEEECCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKR
HHHHHHHHCCCCCCCCCHHHCCEEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCC
AAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWA
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEEEE
NDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
CCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEEE
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRNGKNFGLYRTRM
EECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHEEEEEEEECCCCCCCCEEEHHC
RDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAY
CCHHHHHHHCCCCCCEEEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VTWIAYNPESETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLW
EEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEE
IVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEE
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGV
EEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEE
AINARPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDN
EECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCC
VTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFK
CCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
SYEGVAMEYAFLVALAQGTLPATPAD
CCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDR
CCCCCCCCCEEEEECCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWEILLDANKR
HHHHHHHHCCCCCCCCCHHHCCEEEEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCC
AAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWA
HHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEEEE
NDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
CCCEEEEEEECCCCEEECHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEEE
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRNGKNFGLYRTRM
EECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCHHEEEEEEEECCCCCCCCEEEHHC
RDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAY
CCHHHHHHHCCCCCCEEEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VTWIAYNPESETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLW
EEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCEEE
IVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEE
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGV
EEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEE
AINARPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDN
EECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCC
VTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFK
CCHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
SYEGVAMEYAFLVALAQGTLPATPAD
CCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1769955; 9097040; 9278503 [H]