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The map label for this gene is yebU [H]

Identifier: 157161302

GI number: 157161302

Start: 1939194

End: 1940639

Strand: Direct

Name: yebU [H]

Synonym: EcHS_A1926

Alternate gene names: 157161302

Gene position: 1939194-1940639 (Clockwise)

Preceding gene: 157161301

Following gene: 157161303

Centisome position: 41.76

GC content: 52.35

Gene sequence:

>1446_bases
ATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACGCAAATGCGCGAAGCGATGCCTTCGACGCTCTC
TTTTGATGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAATCTCCGTTGCTG
ATTTCCTGCAATTAACCGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGC
GACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGCGGCCTGTTTTATATTCAGGAAGCCAGTTCAAT
GTTGCCCGTCGCCGCCTTGTTTGCTGACGGTAATGCACCACAGCGGGTGATGGATGTCGCTGCTGCGCCAGGCTCCAAAA
CGACGCAAATTGCCGCACGGATGAATAACGAAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTA
CATGCCAATATCAGCCGCTGTGGCATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGTGGCAGTGCC
AGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAA
ACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCT
GGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACAGGGAAGAAAACGAAGCCGTTTGCATGTGGCTGAAAGAGACTTA
CCCTGACGCAGTAGAGTTTTTACCACTTGGCGAGCTCTTCCCTGCTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGC
ATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCCATTCCCGCCTTACCC
GCCCCCAAATACAAAGTCGGTAATTTTCCGTTCAGCCCGGTGAAAGATCGCGAAGCTGGACAAATTCGTCAGGCGGCTGC
AGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTG
AAGCCCTGATCGGTAAAGTCCGATTTTCTCGCTTGGGGATTAAACTTGCCGAAACGCACAACAAAGGTTATCGCTGGCAG
CATGAAGCAGTTATTGCCCTTGCCACCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGGAAGCGGAGGAGTGGTA
TCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGACGTGTTGGTTACTTTCCAGCATCAGCCGATTGGTT
TAGCCAAACGGATTGGTTCGCGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGGGACGGGAAACTTTTTACCAGTAAC
GCATGA

Upstream 100 bases:

>100_bases
GAATGGGGTACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCC
TGTTTTTTTGCCAGTGGTAT

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCGGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAATCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
A

Sequences:

>Translated_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
A
>Mature_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIER
DNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALP
APKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
A

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=306, Percent_Identity=37.2549019607843, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI76150623, Length=306, Percent_Identity=37.2549019607843, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=38.3233532934132, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI32698918, Length=160, Percent_Identity=35.625, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI39995082, Length=286, Percent_Identity=27.972027972028, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=27.972027972028, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI270288816, Length=328, Percent_Identity=28.9634146341463, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23199998, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI8922322, Length=323, Percent_Identity=28.7925696594427, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288818, Length=323, Percent_Identity=28.7925696594427, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=30, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=97.7035490605428, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=220, Percent_Identity=32.2727272727273, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=319, Percent_Identity=33.2288401253918, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=191, Percent_Identity=31.9371727748691, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=26.7080745341615, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=36.8243243243243, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=31.0679611650485, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI22024126, Length=305, Percent_Identity=35.7377049180328, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355201, Length=207, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53508; Mature: 53508

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
EEEEEHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEHHCCCCHHHHCCCHHHCCCHHHHHCCCCCEEECCEEE
DCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLW
CCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALATPDNVNAFELTP
ECCCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEECCEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C
>Mature Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
EEEEEHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEHHCCCCHHHHCCCHHHCCCHHHHHCCCCCEEECCEEE
DCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLW
CCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALATPDNVNAFELTP
ECCCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEECCEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA