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The map label for this gene is dmlR [H]

Identifier: 157161263

GI number: 157161263

Start: 1898863

End: 1899786

Strand: Reverse

Name: dmlR [H]

Synonym: EcHS_A1887

Alternate gene names: 157161263

Gene position: 1899786-1898863 (Counterclockwise)

Preceding gene: 157161268

Following gene: 157161262

Centisome position: 40.91

GC content: 52.49

Gene sequence:

>924_bases
ATGAATAATTTACCGCTGCTGAATGATTTGCGCGTCTTTATGCTGGTGGCTCGCCGGGCTGGTTTTGCCGCCGTGGCGGA
AGAACTGGGCGTTTCACCGGCGTTCGTCAGCAAGCGCATCGCCTTGCTGGAGCAAACGCTAAACGTGGTGTTGTTACACC
GCACCACCCGCCGCGTCACCATTACCGAAGAAGGAGAGCGGATTTATGAATGGGCGCAGCGGATTTTGCAGGATGTCGGT
CAGATGATGGACGAACTTTCTGACGTGCGTCAGGTGCCGCAGGGGATGCTGCGTATTATCAGCAGCTTTGGTTTTGGACG
ACAGGTGGTGGCTCCGGCGTTATCAGCGCTGGCGAAGGCGTATCCGCAACTGGAATTACGTTTTGATGTGGAAGACCGAC
TGGTCGATTTGGTCAATGAAGGTGTCGATCTCGATATTCGCATTGGCGATGACATCGCCCCTAATTTAATCGCCCGCAAA
CTGGCGACCAATTACCGTATTCTCTGCGCGTCGCCGGAATTTATTGCGCAACATGGCGCGCCGAAACATTTAACCGATCT
TTCTGCATTGCCGTGCCTGGTCATTAAAGAGCGCGATCATCCTTTTGGTGTCTGGCAATTACGCAATAAAGAAGGCCCCC
ATGCGATTAAAGTCACCGGCCCACTGTCGTCTAATCATGGAGAAATTGTCCATCAGTGGTGTCTCGATGGGCAGGGGATT
GCTCTGCGTTCCTGGTGGGATGTTAGCGAAAACATTGCCAGTGGTCATTTAGTGCAAGTGCTACCGGAATATTACCAGCC
AGCGAACGTCTGGGCCGTTTATGTTTCAAGGCTGGCGACGTCAGCGAAAGTGCGGATAACGGTAGAATTTTTACGCCAGT
ATTTTGCCGAGCACTACCCGAATTTTTCACTGGAGCATGCCTGA

Upstream 100 bases:

>100_bases
GGTTCTCACTTCCTGACCATCAAAATTGACAACATCACCATCCTATAATTGACTGAAAAGGAATTAATCCCCGTAACTGG
TGAAACATAAAACACGAATC

Downstream 100 bases:

>100_bases
TTAATGATTCAATTATCGGGTTGATATCAGGTTAAAACCTGATTTTCTCCTTTCTAAGCCGCTACAGATTGGTTAGCATA
TTCACCTTTAATCGCGCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVG
QMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA

Sequences:

>Translated_307_residues
MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVG
QMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA
>Mature_307_residues
MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVG
QMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKVTGPLSSNHGEIVHQWCLDGQGI
ALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYPNFSLEHA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=307, Percent_Identity=99.6742671009772, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=49.1408934707904, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=33.5640138408304, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.0276816608997, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.6928327645051, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=29.4314381270903, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=266, Percent_Identity=24.812030075188, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789173, Length=257, Percent_Identity=31.5175097276265, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788706, Length=193, Percent_Identity=30.5699481865285, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786448, Length=257, Percent_Identity=29.1828793774319, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=28.9940828402367, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786713, Length=117, Percent_Identity=37.6068376068376, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1789492, Length=266, Percent_Identity=27.0676691729323, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34605; Mature: 34605

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCEEEE
ITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARKLATNYRILCASPEFIAQHGA
CCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHCCC
PKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKVTGPLSSNHGEIVHQWCLDGQGI
CCHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYP
EEEEHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCC
NFSLEHA
CCCCCCC
>Mature Secondary Structure
MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLEQTLNVVLLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCEEEE
ITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISSFGFGRQVVAPALSALAKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARKLATNYRILCASPEFIAQHGA
CCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHCCC
PKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKVTGPLSSNHGEIVHQWCLDGQGI
CCHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVSRLATSAKVRITVEFLRQYFAEHYP
EEEEHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCC
NFSLEHA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]