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The map label for this gene is spy

Identifier: 157161203

GI number: 157161203

Start: 1843222

End: 1843707

Strand: Reverse

Name: spy

Synonym: EcHS_A1825

Alternate gene names: 157161203

Gene position: 1843707-1843222 (Counterclockwise)

Preceding gene: 157161205

Following gene: 157161202

Centisome position: 39.7

GC content: 50.82

Gene sequence:

>486_bases
ATGCGTAAATTAACTGCACTGTTTGTTGCCTCTACCCTGGCTCTTGGCGCGGCTAACCTGGCCCATGCCGCAGACACCAC
TACCGCAGCACCGGCTGACGCGAAGCCGATGATGCACCACAAAGGCAAGTTCGGTCCGCATCAGGACATGATGTTCAAAG
ACCTGAACCTGACCGACGCGCAGAAACAGCAGATCCGCGAAATCATGAAAGGCCAGCGTGACCAGATGAAACGTCCGCCG
CTGGAAGAACGCCGCGCAATGCATGACATCATTGCCAGCGATACCTTCGATAAAGTAAAAGCTGAAGCGCAGATCGCAAA
AATGGAAGAACAGCGCAAAGCTAACATGCTGGCGCACATGGAAACCCAGAACAAAATTTACAACATCCTGACGCCGGAAC
AGAAAAAGCAATTTAATGCTAATTTTGAGAAGCGTCTGACAGAACGTCCAGCGGCAAAAGGTAAAATGCCTGCAACTGCT
GAATAA

Upstream 100 bases:

>100_bases
CTCTGATTAATTGACGCTAAAGTCAGTAAAGTTAATCTCGTCAACACGGCACGCTACTTAAGAAAGCCGTAATAAATAAC
TGAAAGGAAGGATATAGAAT

Downstream 100 bases:

>100_bases
TCTTTCAGCCAAAAAACTTAAGACCGCCGGTCTTGTCCACTACCTTGCAGTAATGCGGTGGACAGGATCGGCGGTTTTCT
TTTCTCTTCTCAAACAGTTG

Product: periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPP
LEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
E

Sequences:

>Translated_161_residues
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPP
LEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
E
>Mature_161_residues
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPP
LEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
E

Specific function: Could be involved in zinc resistance or another aspect of zinc metabolism

COG id: COG3678

COG function: function code UNTP; P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli CpxP

Homologues:

Organism=Escherichia coli, GI1788039, Length=161, Percent_Identity=100, Blast_Score=327, Evalue=2e-91,
Organism=Escherichia coli, GI48994991, Length=148, Percent_Identity=30.4054054054054, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPY_ECOLI (P77754)

Other databases:

- EMBL:   Y07714
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G64933
- RefSeq:   AP_002362.1
- RefSeq:   NP_416257.1
- ProteinModelPortal:   P77754
- SMR:   P77754
- IntAct:   P77754
- STRING:   P77754
- EnsemblBacteria:   EBESCT00000001193
- EnsemblBacteria:   EBESCT00000017535
- GeneID:   946253
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1732
- KEGG:   eco:b1743
- EchoBASE:   EB3263
- EcoGene:   EG13490
- eggNOG:   COG3678
- GeneTree:   EBGT00050000010082
- HOGENOM:   HBG679676
- OMA:   MMFKGLN
- ProtClustDB:   PRK10455
- BioCyc:   EcoCyc:G6939-MONOMER
- Genevestigator:   P77754
- InterPro:   IPR012899

Pfam domain/function: PF07813 LTXXQ

EC number: NA

Molecular weight: Translated: 18199; Mature: 18199

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCHH
QKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHM
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE
HHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCCCHH
QKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHM
HHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE
HHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9068658; 9097039; 9278503; 9694902