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The map label for this gene is chbF [H]

Identifier: 157161195

GI number: 157161195

Start: 1835229

End: 1836581

Strand: Reverse

Name: chbF [H]

Synonym: EcHS_A1816

Alternate gene names: 157161195

Gene position: 1836581-1835229 (Counterclockwise)

Preceding gene: 157161196

Following gene: 157161194

Centisome position: 39.55

GC content: 46.93

Gene sequence:

>1353_bases
ATGAGCCAGAAATTAAAAGTCGTCACTATTGGTGGCGGGAGCAGCTATACCCCGGAGTTACTGGAAGGATTTATTAAGCG
TTATCACGAATTGCCGGTCAGCGAATTATGGCTGGTGGATGTCGAAGGTGGTAAAGCGAAACTGGATATTATTTTCGATC
TCTGCCAACGGATGATTGATAACGCTGGCGTCCCGATGAAGCTTTATAAAACGCTGGATCGCCGCGAAGCATTGAAAGAT
GCTGATTTCGTTACTACCCAACTGCGCGTTGGCCAATTACCGGCGCGTGAACTGGATGAACGTATTCCATTAAGTCATGG
TTATCTTGGTCAGGAAACCAACGGCGCGGGCGGTTTGTTTAAAGGTCTGCGTACCATTCCGGTGATTTTTGACATCGTAA
AAGATGTCGAAGAACTTTGTCCGAATGCATGGGTGATTAACTTCACTAACCCGGCGGGAATGGTCACTGAAGCCGTTTAT
CGTCATACCGGATTTAAACGCTTTATCGGCGTGTGTAATATTCCGATCGGCATGAAGATGTTTATTCACGATGTTCTGAT
GCTGAAAGACAGCGATGATTTATCTATCGATTTGTTCGGCCTCAACCATATGGTGTTCATTAAGGATGTGCTGGTAAATG
GCAAGTCGCGCTTTGCCGAATTGCTTGATGGTGTGGCGTCAGGGCAGTTAAAAGCGTCCTCTGTAAAAAATATTTTCGAT
CTGCCATTTAGTGAGGGCTTAATTCGTTCGTTGAATCTGCTGCCATGTTCTTATCTGCTGTATTACTTCAAGCAGAAAGA
GATGCTGGCTATTGAAATGGGCGAATACTACAAAGGCGGCGCACGAGCGCAGGTAGTACAGAAAGTCGAGAAACAACTTT
TTGAGCTGTATAAAAACCCGGAGTTGAAAGTTAAGCCGAAAGAACTGGAACAGCGCGGTGGGGCTTATTACTCTGATGCA
GCGTGCGAAGTGATCAACGCTATCTACAACGACAAGCAAGCAGAACATTACGTTAATATCCCGCATCATGGGCATATTGA
TAATATTCCGGCAGACTGGGCGGTAGAAATGACCTGTAAGCTGGGGCGCGATGGCGCGACGCCACATCCGCGCATTACGC
ATTTCGATGATAAAGTAATGGGGCTGATTCACACCATTAAAGGCTTCGAGATTGCTGCCAGTAACGCCGCACTTAGCGGA
GAATTTAACGATGTGTTACTGGCGCTAAACCTTAGTCCGTTGGTGCATTCCGATCGCGATGCTGAGCTGCTGGCACGCGA
GATGATTCTGGCGCACGAGAAATGGCTGCCAAACTTTGCCGACTGCATCGCAGAGCTTAAAAAAGCACATTAA

Upstream 100 bases:

>100_bases
TATACCTGACAATTCACATATCTGTCCTGTTGCTGGATTATTTATGTCCGGGGGGGGCAGATATGCCAGATATCAGTATT
CTGTACTGAAGGGAGAAATT

Downstream 100 bases:

>100_bases
CCGAGGCTGATTATGGAACGCTTACTGATTGTTAATGCCGATGATTTTGGCTTAAGCAAAGGTCAGAACTACGGCATTAT
CGAGGCCTGTCGCAATGGGA

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: Cellobiose-6-phosphate hydrolase; Phospho-chitobiase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKD
ADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASSVKNIFD
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDA
ACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSG
EFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH

Sequences:

>Translated_450_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKD
ADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASSVKNIFD
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDA
ACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSG
EFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
>Mature_449_residues
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDA
DFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYR
HTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASSVKNIFDL
PFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAA
CEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGE
FNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH

Specific function: Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl- beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=450, Percent_Identity=99.1111111111111, Blast_Score=921, Evalue=0.0,
Organism=Escherichia coli, GI1790560, Length=459, Percent_Identity=25.4901960784314, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50463; Mature: 50332

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMID
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
NAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASSVKNIFD
HHHHHHHEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHC
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCK
CCEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEEE
LGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRD
CCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEECCCEECCCCCCEEEEEECCHHHCCCCC
AELLAREMILAHEKWLPNFADCIAELKKAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMID
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
NAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASSVKNIFD
HHHHHHHEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHC
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCK
CCEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEEE
LGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRD
CCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEECCCEECCCCCCEEEEEECCHHHCCCCC
AELLAREMILAHEKWLPNFADCIAELKKAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 8121401; 10572139; 9405618; 10913117 [H]