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The map label for this gene is malI

Identifier: 157161081

GI number: 157161081

Start: 1720105

End: 1721133

Strand: Reverse

Name: malI

Synonym: EcHS_A1695

Alternate gene names: 157161081

Gene position: 1721133-1720105 (Counterclockwise)

Preceding gene: 157161085

Following gene: 157161080

Centisome position: 37.07

GC content: 53.94

Gene sequence:

>1029_bases
ATGGCTACCGCCAAAAAAATAACCATTCATGATGTTGCGCTGGCTGCGGGCGTGTCGGTAAGTACCGTTTCGCTGGTGCT
TAGTGGCAAAGGGCGAATCTCTACCGCCACAGGAGAACGCGTTAACGCCGCCATTGAAGAGCTGGGATTTGTGCGCAATC
GCCAGGCGTCGGCGCTGCGCGGCGGGCAAAGCGGCGTCATTGGTTTGATCGTCCGTGATTTATCTGCGCCGTTTTACGCC
GAATTGACGGCCGGATTGACGGAAGCTCTGGAAGCGCAGGGACGGATGGTTTTTTTGCTTCACGGCGGTAAAGACGGTGA
GCAGCTGGCACAGCGGTTTTCACTGTTACTGAATCAGGGTGTCGATGGTGTGGTAATTGCCGGAGCAGCAGGAAGTAGCG
ATGACCTGCGACGGATGGCAGAAGAAAAAGCTATCCCGGTGATTTTCGCTTCCCGTGCCAGTTATCTTGATGATGTTGAT
ACGGTTCGCCCGGACAACATGCAGGCTGCACAGTTGTTGACGGAGCATCTCATTCGCAATGGGCATCAGCGGATCGCCTG
GCTGGGAGGGCAAAGTTCCTCATTAACCCGTGCAGAACGGGTTGGGGGCTATTGTGCAACTCTACTAAAATTTGGCCTGC
CGTTTCATAGCGATTGGGTGTTGGAGTGCACTTCCAGCCAGAAGCAAGCCGCGGAAGCTATCACGGCGCTTTTACGTCAT
AACCCGACCATCAGTGCCGTGGTTTGCTATAACGAAACTATTGCGATGGGGGCATGGTTTGGTTTGCTCAAAGCAGGGCG
GCAAAGCGGGGAAAGCGGAGTCGATCGTTACTTTGAGCAACAGGTTTCGCTGGCGGCATTTACCGATGCGACACCAACCA
CACTTGATGATATACCTGTTACCTGGGCCAGCACGCCAGCGCGGGAACTTGGTATCACACTTGCGGATCGCATGATGCAA
AAAATCACCCATGAAGAGACGCATTCACGCAATCTTATTATTCCCGCCCGGCTCATTGCAGCGAAATAA

Upstream 100 bases:

>100_bases
GTGAGGCATAAATCACATTACGCAACGATAATAGCGGGTATAAGATAAATAAAAGGTAAAACGTTTTATCTGTCACATAA
TCAGGGAGTAGGTCATCTGC

Downstream 100 bases:

>100_bases
TCCTCTCTTTATCTGCTATACCTGGTAGTGTCCTTTCCTCAAGGTTAATGATAAAAAATGTGGCACACAGGCGGTGCCTT
TTATTTTTACTGAAGTTCAC

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQ
KITHEETHSRNLIIPARLIAAK

Sequences:

>Translated_342_residues
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQ
KITHEETHSRNLIIPARLIAAK
>Mature_341_residues
ATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAE
LTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDT
VRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRHN
PTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQK
ITHEETHSRNLIIPARLIAAK

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=100, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=25.8461538461538, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=27.8787878787879, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=29.0220820189274, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=26.2658227848101, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788474, Length=261, Percent_Identity=29.8850574712644, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=28.3737024221453, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=26.2390670553936, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=26.1980830670927, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=23.2835820895522, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790715, Length=334, Percent_Identity=24.8502994011976, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790689, Length=258, Percent_Identity=27.5193798449612, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MALI_ECOLI (P18811)

Other databases:

- EMBL:   M28539
- EMBL:   M60722
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64918
- RefSeq:   AP_002241.1
- RefSeq:   NP_416137.1
- ProteinModelPortal:   P18811
- SMR:   P18811
- DIP:   DIP-10143N
- STRING:   P18811
- EnsemblBacteria:   EBESCT00000000253
- EnsemblBacteria:   EBESCT00000018376
- GeneID:   947104
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1612
- KEGG:   eco:b1620
- EchoBASE:   EB0552
- EcoGene:   EG10557
- eggNOG:   COG1609
- GeneTree:   EBGT00050000009236
- HOGENOM:   HBG753640
- OMA:   WVLECES
- ProtClustDB:   PRK10014
- BioCyc:   EcoCyc:PD00361
- Genevestigator:   P18811
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36625; Mature: 36494

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCCEEEHHHHHHHCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGITLADRMMQKITHEETHSRNLIIPARLIAAK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC
>Mature Secondary Structure 
ATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCEEEHHHHHHHCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGITLADRMMQKITHEETHSRNLIIPARLIAAK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503