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The map label for this gene is pntA

Identifier: 157161063

GI number: 157161063

Start: 1698506

End: 1700038

Strand: Reverse

Name: pntA

Synonym: EcHS_A1677

Alternate gene names: 157161063

Gene position: 1700038-1698506 (Counterclockwise)

Preceding gene: 157161068

Following gene: 157161062

Centisome position: 36.61

GC content: 52.45

Gene sequence:

>1533_bases
ATGCGAATTGGCATACCAAGAGAACGGTTAACCAATGAAACCCGTGTTGCAGCAACGCCAAAAACAGTGGAACAGCTGCT
GAAACTGGGTTTTACCGTCGCGGTAGAGAGCGGCGCGGGTCAACTGGCAAGTTTTGACGATAAAGCGTTTGTGCAAGCGG
GCGCTGAAATTGTAGAAGGGAATAGCGTCTGGCAGTCAGAGATCATTCTGAAGGTCAATGCGCCGTTAGATGATGAAATT
GCGTTACTGAATCCTGGGACAACGCTGGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAATTAATGCAAAAACTTGCGGA
ACGTAACGTGACCGTGATGGCGATGGACTCTGTGCCGCGTATCTCACGCGCACAATCGCTGGACGCACTAAGCTCGATGG
CGAACATCGCCGGTTATCGCGCCATTGTTGAAGCGGCACATGAATTTGGGCGCTTCTTTACCGGGCAAATTACTGCGGCC
GGGAAAGTGCCACCGGCAAAAGTGATGGTGATTGGTGCGGGTGTTGCAGGTCTGGCCGCCATTGGCGCAGCAAACAGTCT
CGGCGCGATTGTGCGTGCATTCGACACCCGCCCGGAAGTGAAAGAACAAGTTCAAAGTATGGGCGCGGAATTCCTCGAGC
TGGATTTTAAAGAGGAAGCTGGCAGCGGCGATGGCTATGCCAAAGTGATGTCGGACGCGTTCATCAAAGCGGAAATGGAA
CTCTTTGCCGCCCAGGCAAAAGAGGTCGATATCATTGTCACCACCGCGCTTATTCCAGGCAAACCAGCGCCGAAGCTAAT
TACCCGTGAAATGGTTGACTCCATGAAGGCGGGCAGTGTGATTGTCGACCTGGCAGCCCAAAACGGCGGCAACTGTGAAT
ACACCGTGCCGGGTGAAATCTTCACTACGGAAAATGGTGTCAAAGTGATTGGTTATACCGATCTTCCGGGCCGTCTGCCG
ACGCAATCCTCACAGCTTTACGGCACAAACCTCGTTAATCTGCTGAAACTGTTGTGCAAAGAGAAAGACGGCAATATCAC
TGTTGATTTTGATGATGTGGTGATTCGCGGCGTGACCGTGATCCGTGCGGGCGAAATTACCTGGCCGGCACCGCCGATTC
AGGTATCAGCTCAGCCGCAGGCGGCACAAAAAGCGGCACCGGAAGTGAAAACTGAGGAAAAATGTACCTGCTCACCGTGG
CGTAAATACGCGTTGATGGCGCTGGCAATCATTCTTTTTGGCTGGATGGCAAGCGTTGCGCCGAAAGAATTCCTTGGGCA
CTTCACCGTTTTCGCGCTGGCCTGCGTTGTCGGTTATTACGTGGTGTGGAATGTATCGCACGCGCTGCATACACCGTTGA
TGTCGGTCACCAACGCGATTTCAGGGATTATTGTTGTCGGAGCACTGTTGCAGATTGGCCAGGGCGGCTGGGTTAGCTTC
CTTAGTTTTATCGCGGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGCATGCTGAAAATGTT
CCGCAAAAATTAA

Upstream 100 bases:

>100_bases
ATTTATTTTAACGGAGTAACATTTAGCTCGTACATGAGCAGCTTGTGTGGCTCCTGACACAGGCAAACCATCATCAATAA
AACCGATGGAAGGGAATATC

Downstream 100 bases:

>100_bases
GGGGTAACATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGTCTGGCCGGTC
TTTCGAAACATGAAACGTCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=50.5639097744361, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=50.5639097744361, Blast_Score=500, Evalue=1e-141,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=100, Blast_Score=1038, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=50.5769230769231, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNTA_ECOLI (P07001)

Other databases:

- EMBL:   X04195
- EMBL:   X66086
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S24380
- RefSeq:   AP_002224.1
- RefSeq:   NP_416120.1
- PDB:   1X13
- PDB:   1X14
- PDB:   1X15
- PDB:   2BRU
- PDBsum:   1X13
- PDBsum:   1X14
- PDBsum:   1X15
- PDBsum:   2BRU
- ProteinModelPortal:   P07001
- SMR:   P07001
- DIP:   DIP-366N
- MINT:   MINT-1299705
- STRING:   P07001
- EnsemblBacteria:   EBESCT00000001358
- EnsemblBacteria:   EBESCT00000017035
- GeneID:   946628
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1595
- KEGG:   eco:b1603
- EchoBASE:   EB0737
- EcoGene:   EG10744
- eggNOG:   COG3288
- GeneTree:   EBGT00050000011851
- HOGENOM:   HBG289735
- OMA:   FKVNAPT
- ProtClustDB:   PRK09424
- BioCyc:   EcoCyc:PNTA-MONOMER
- Genevestigator:   P07001
- GO:   GO:0005488
- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571
- TIGRFAMs:   TIGR00561

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N

EC number: =1.6.1.2

Molecular weight: Translated: 54624; Mature: 54624

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00836 ALADH_PNT_1; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1487dc74)-; HASH(0x14e7f3cc)-; HASH(0x1422f3cc)-; HASH(0x147d9280)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCHHHCCC
NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR
CCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEE
FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCEEE
IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVA
EEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCH
>Mature Secondary Structure
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCHHHCCC
NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR
CCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI
HHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEE
FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCEEE
IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVA
EEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078