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The map label for this gene is ynfL

Identifier: 157161055

GI number: 157161055

Start: 1690834

End: 1691727

Strand: Reverse

Name: ynfL

Synonym: EcHS_A1669

Alternate gene names: 157161055

Gene position: 1691727-1690834 (Counterclockwise)

Preceding gene: 157161059

Following gene: 157161054

Centisome position: 36.43

GC content: 52.68

Gene sequence:

>894_bases
ATGAATATTGAACTTCGTCATCTGCGTTACTTTGTTGCTGTTGCGGAAGAGCTGCATTTCGGGCGCGCCGCTGCCCGCCT
GAATATTTCGCAACCGCCGCTAAGTCAGCAGATTCAGGCGCTGGAGCAACAAATTGGTGCCCGACTGCTGGCACGAACCA
ATCGCAGTGTATTGCTGACGGCAGCAGGAAAACAGTTTCTTGCAGATAGTCGGCAAATCCTGTCTATGGTGGATGACGCT
GCCGCTCGCGCTGAAAGGCTGCATCAGGGTGAAGCGGGGGAGTTGCGCATTGGTTTTACTTCGTCGGCTCCTTTTATTCG
GGCGGTGTCCGATACGTTATCGCTGTTTCGCCGTGATTATCCTGATGTCCATTTACAAACCCGCGAAATGAACACTCGCG
AGCAAATCGCTCCGCTCATTGAAGGAACGCTGGATATGGGATTGCTGCGTAACACAGCGTTACCGGAGTCGCTTGAACAC
GCAGTCATCGTCCATGAACCGCTTATGGCGATGATCCCGCACGATCATCCCCTGGCAAATAACCCGAATGTAACGCTGGC
TGAACTGGCGAAAGAACCCTTTGTCTTTTTTGATCCGCACGTCGGGACAGGGCTGTATGACGATATTCTCGGGCTGATGC
GACGTTACCATTTGACGCCCGTCATCACTCAGGAGGTGGGCGAGGCAATGACCATCATCGGTCTGGTTTCCGCCGGTCTG
GGTGTTTCAATTTTGCCTGCGTCATTTAAACGTGTTCAGCTCAACGAAATGCGCTGGGTGCCGATTGCTGAAGAGGATGC
GGTTTCTGAAATGTGGTTGGTCTGGCCGAAACATCATGAACAAAGTCCGGCTGCGCGTAACTTTCGTATTCATCTGCTGA
ATGCTCTCAGGTGA

Upstream 100 bases:

>100_bases
TCCTTGCTAAATATGCCTGTAGATCAGGCTTATACATAGGGTAGGAAAATCGAATTGTTCTGTCTAATATATTAATAATC
TCAAATAAGATGTTTTAAAT

Downstream 100 bases:

>100_bases
GGGAAATTTCAGCGAAAAAGCCCGAAAAATGTGCTGTTAATCACATGCCTAAGTAAAAATTTGACGACACGTATTGAAGT
GCTTCACCATAGCCTACAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Sequences:

>Translated_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
>Mature_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=100, Blast_Score=602, Evalue=1e-174,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=36.3636363636364, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=37.7431906614786, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=31.8548387096774, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=29.1497975708502, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=29.7945205479452, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=32.8205128205128, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=26.7657992565056, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786713, Length=258, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=25.2491694352159, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=43.6170212765958, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789506, Length=284, Percent_Identity=22.887323943662, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789204, Length=251, Percent_Identity=26.2948207171315, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=32.3076923076923, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=24.7491638795987, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=163, Percent_Identity=32.5153374233129, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.962457337884, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YNFL_ECOLI (P77559)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64915
- RefSeq:   AP_002216.1
- RefSeq:   NP_416112.1
- ProteinModelPortal:   P77559
- SMR:   P77559
- DIP:   DIP-12771N
- STRING:   P77559
- EnsemblBacteria:   EBESCT00000001120
- EnsemblBacteria:   EBESCT00000017281
- GeneID:   946136
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1587
- KEGG:   eco:b1595
- EchoBASE:   EB3611
- EcoGene:   EG13850
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008327
- HOGENOM:   HBG685425
- OMA:   SRNFRIH
- ProtClustDB:   CLSK880141
- BioCyc:   EcoCyc:G6853-MONOMER
- Genevestigator:   P77559
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33282; Mature: 33282

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503