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The map label for this gene is rspB [H]

Identifier: 157161039

GI number: 157161039

Start: 1675184

End: 1676050

Strand: Reverse

Name: rspB [H]

Synonym: EcHS_A1653

Alternate gene names: 157161039

Gene position: 1676050-1675184 (Counterclockwise)

Preceding gene: 157161040

Following gene: 157161038

Centisome position: 36.09

GC content: 47.17

Gene sequence:

>867_bases
ATGAAAAGCATATTAATTGAAAAACCGAATCAACTGGCGATTGTCGAACGTGAAATACCCACCCCGTCAGCGGGTGAAGT
ACGAGTAAAAGTGAAACTTGCCGGAATTTGTGGTTCAGATAGCCATATTTATCGTGGGCATAATCCTTTTGCGAAATATC
CGCGCGTCATTGGTCATGAATTCTTTGGCGTCATTGATGCAGTGGGTGAAGGCGTGGAAAGCGCCAGAGTCGGTGAACGT
GTTGCTGTCGATCCGGTGGTCAGCTGTGGGCATTGCTATCCGTGCTCTGTAGGTAAACCGAACGTTTGTACGACACTGGC
TGTATTAGGTGTGCACGCTGACGGTGGTTTCAGTGAATATGCCGTGGTTCCGGCAAAAAATGCGTGGAAAATTCCTGAAG
CAGTGGCCGATCAATATGCGGTAATGATCGAACCTTTTACCATTGCGGCTAACGTAACCGGACATGGTCAACCGACTGAA
AATGATACCGTTCTGGTTTATGGTGCCGGTCCAATCGGCCTGACGATCGTTCAGGTATTAAAAGGCGTCTATAACGTTAA
AAATGTGATTGTTGCCGATCGCATTGATGAACGACTGGAAAAAGCGAAAGAGAGCGGGGCTGACTGGGCGATTAATAACA
GCCAGACACCGCTTGGCGAGATTTTCACTGAAAAAGGCATCAAGCCGACATTAATTATTGATGCGGCTTGTCATCCTTCT
ATCCTGAAAGAGGCCGTAACGCTGGCTTCTCCAGCGGCACGTATTGTATTGATGGGTTTCTCCAGTGAACCGTCTGAAGT
GATTCAGCAAGGAATTACCGGAAAAGAACTCTCTATTTTCGGTGATGCTGCCAACTTACTGATTTAG

Upstream 100 bases:

>100_bases
TCGATGAAAAGCTGGCGGCGAAATATCCCTATGAACCTGCTTATCTACCAGTCGCACGTCTGGAAGATGGCACGCTGTGG
AACTGGTAAGGAGTAAGATA

Downstream 100 bases:

>100_bases
TGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGG
TGCGTAACGGCAAAAGCACC

Product: starvation sensing protein RspB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGER
VAVDPVVSCGHCYPCSVGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGEIFTEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFGDAANLLI

Sequences:

>Translated_288_residues
MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGER
VAVDPVVSCGHCYPCSVGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGEIFTEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFGDAANLLI
>Mature_288_residues
MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGER
VAVDPVVSCGHCYPCSVGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGEIFTEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFGDAANLLI

Specific function: Not known; probable catabolic enzyme [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=287, Percent_Identity=29.616724738676, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI262073058, Length=248, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI71743840, Length=248, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4501933, Length=251, Percent_Identity=29.8804780876494, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4501929, Length=251, Percent_Identity=29.0836653386454, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI71565154, Length=240, Percent_Identity=30, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI156523966, Length=258, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4501939, Length=258, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI34577061, Length=251, Percent_Identity=28.6852589641434, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1787863, Length=285, Percent_Identity=98.2456140350877, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI226510992, Length=174, Percent_Identity=42.5287356321839, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790045, Length=277, Percent_Identity=31.0469314079422, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1788407, Length=293, Percent_Identity=33.1058020477816, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788073, Length=265, Percent_Identity=28.6792452830189, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790718, Length=288, Percent_Identity=27.0833333333333, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788075, Length=285, Percent_Identity=29.8245614035088, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082125, Length=220, Percent_Identity=34.5454545454545, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87081918, Length=269, Percent_Identity=29.7397769516729, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786825, Length=240, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786552, Length=228, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87082401, Length=110, Percent_Identity=36.3636363636364, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=270, Percent_Identity=32.962962962963, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17562878, Length=208, Percent_Identity=31.7307692307692, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25146526, Length=241, Percent_Identity=29.8755186721992, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71997431, Length=243, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=126, Percent_Identity=34.1269841269841, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565904, Length=213, Percent_Identity=29.1079812206573, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=122, Percent_Identity=28.6885245901639, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=219, Percent_Identity=32.8767123287671, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319955, Length=219, Percent_Identity=32.8767123287671, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323099, Length=224, Percent_Identity=30.3571428571429, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319258, Length=222, Percent_Identity=31.0810810810811, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319257, Length=212, Percent_Identity=28.7735849056604, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=272, Percent_Identity=27.2058823529412, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=134, Percent_Identity=34.3283582089552, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=133, Percent_Identity=32.3308270676692, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=132, Percent_Identity=32.5757575757576, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137530, Length=205, Percent_Identity=31.219512195122, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17737897, Length=206, Percent_Identity=30.5825242718447, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17737895, Length=201, Percent_Identity=27.363184079602, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221457811, Length=266, Percent_Identity=23.3082706766917, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550770, Length=266, Percent_Identity=23.3082706766917, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551930, Length=266, Percent_Identity=23.3082706766917, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 30534; Mature: 30534

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHE
CCCCEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHH
FFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSVGKPNVCTTLAVLGVHADGGFSEY
HHHHHHHHHCCHHHHHCCCEEEECCHHHCCCEEECCCCCCCHHHEEEEEEEECCCCCCCE
AVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTENDTVLVYGAGPIGLTIVQVL
EEEECCCCCCCCHHHHCCEEEEEECEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGEIFTEKGIKPTLIIDAACHPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCEEEEEECCCCHH
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFGDAANLLI
HHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEECCHHHHCC
>Mature Secondary Structure
MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHE
CCCCEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHH
FFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSVGKPNVCTTLAVLGVHADGGFSEY
HHHHHHHHHCCHHHHHCCCEEEECCHHHCCCEEECCCCCCCHHHEEEEEEEECCCCCCCE
AVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTENDTVLVYGAGPIGLTIVQVL
EEEECCCCCCCCHHHHCCEEEEEECEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGEIFTEKGIKPTLIIDAACHPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCEEEEEECCCCHH
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFGDAANLLI
HHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]