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The map label for this gene is cynR [H]

Identifier: 157159855

GI number: 157159855

Start: 422997

End: 423896

Strand: Reverse

Name: cynR [H]

Synonym: EcHS_A0403

Alternate gene names: 157159855

Gene position: 423896-422997 (Counterclockwise)

Preceding gene: 157159859

Following gene: 157159848

Centisome position: 9.13

GC content: 55.22

Gene sequence:

>900_bases
ATGCTCTCTCGACATATCAATTATTTTCTTGCCGTGGCTGAACATGGCAGCTTCACCCGTGCCGCCAGTGCGTTGCACGT
CTCTCAGCCTGCGCTTTCCCAGCAGATTCGCCAGTTAGAGGAGAGTTTAGGCGTGCCGCTGTTTGACCGTAGCGGGCGAA
CGATTCGTCTCACCGATGCAGGAGAAGTCTGGCGACAGTACGCCAGCCGGGCGTTACAGGAACTGGGAGCGGGTAAACGG
GCGATTCATGATGTTGCCGATCTGACGCGAGGATCGCTGCGTATCGCCGTCACCCCCACCTTTACAAGCTACTTTATCGG
CCCTTTAATGGCGGATTTCTATGCGCGCTACCCCGGCATCACGCTCCAGCTACAGGAAATGTCGCAGGAGAAAATCGAAG
ATCTGCTTTGTCGCGACGAGCTGGACGTTGGGATTGCCTTCGCGCCTGTGCATTCGCCGGAGCTGGAGGCAATTCCTTTA
CTGACAGAAAGTTTAGCGTTAGTCGTGGCGCAACATCATCCGCTGGCTGCCTGTGAACAGGTGGCGTTGAGTCGCTTGCA
TGATGAAAAACTGGTCCTGCTCAGCGCGGAATTTGCCACCAGAGAGCAAATTGACCACTACTGCGAGAAAGCGGGGCTAC
ATCCACAGGTGGTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTTCCACATTGTTA
CCAGCAGCGATTGCCACACAACATGACGGGCTTAAAGCGATTTCTCTTGCCCCGCCACTACTGGAGAGAACGGCGGTTTT
GTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGCGAAGGCATTTTTGCACATGGCGTTGGAGGAATGTGCGGATGTTG
GCGAGAATGAATCACGGTAG

Upstream 100 bases:

>100_bases
CTGTCTGTCTCTGGAATGAGAGGCAGACTACTCGCCGATTGTCATAAGGTAAAAGTCTCATTTATGATGAGTTCTATTGG
ATTTACTTATAGGTTGGCGA

Downstream 100 bases:

>100_bases
CCACTGACACAGGTCAGTGGCTCTTCAACACAGAGGAAATTACCGTTTGTAATCGATGGCTTCTGGCTGCTCCAGATATA
CGGTGGTTTGTGCCGGTTGT

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR

Sequences:

>Translated_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
>Mature_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=97.3244147157191, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1790399, Length=276, Percent_Identity=30.7971014492754, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=29.4520547945205, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=28.0821917808219, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787530, Length=235, Percent_Identity=29.7872340425532, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788748, Length=290, Percent_Identity=26.8965517241379, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081904, Length=227, Percent_Identity=33.4801762114537, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367136, Length=227, Percent_Identity=31.2775330396476, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=30.6451612903226, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=25, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786401, Length=171, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=29.7945205479452, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789173, Length=212, Percent_Identity=31.6037735849057, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789204, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788296, Length=207, Percent_Identity=29.4685990338164, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=182, Percent_Identity=31.3186813186813, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790262, Length=236, Percent_Identity=25, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=24.5614035087719, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789639, Length=163, Percent_Identity=31.9018404907975, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787949, Length=168, Percent_Identity=32.7380952380952, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=206, Percent_Identity=29.6116504854369, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=158, Percent_Identity=34.1772151898734, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081684, Length=294, Percent_Identity=23.469387755102, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=222, Percent_Identity=27.4774774774775, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788706, Length=172, Percent_Identity=32.5581395348837, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082184, Length=252, Percent_Identity=25.3968253968254, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=29.1428571428571, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQTAAAKAFLHMALEECADVGENESR
HHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]