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The map label for this gene is prpR [H]

Identifier: 157159848

GI number: 157159848

Start: 412848

End: 414434

Strand: Reverse

Name: prpR [H]

Synonym: EcHS_A0396

Alternate gene names: 157159848

Gene position: 414434-412848 (Counterclockwise)

Preceding gene: 157159855

Following gene: 157159846

Centisome position: 8.92

GC content: 55.26

Gene sequence:

>1587_bases
ATGGCACATCCACCACGGCTTAATGACGACAAACCGGTTATCTGGACGGTATCTGTAACGCGCCTGTTCGAGCTGTTTCG
CGATATCAGCCTCGAGTTTGATCACCTGGCGAACATTACCCCTATCCAGCTTGGCTTTGAAAAAGCAGTGACCTATATCC
GCAAGAAACTGGCAAACGAACGCTGCGACGCCGTCATCGCCGCTGGCTCTAACGGCGCGTACCTGAAAAGCCGCCTGTCA
GTGCCAGTTATTTTGATTAAACCGAGCGGCTACGATGTGTTACAGGCACTGGCAAAAGCCGGAAAGCTCACCTCTTCTAT
CGGCGTTGTCACTTATCAGGAAACTATTCCGGCACTGGTGGCGTTTCAAAAAACCTTTAATTTGCGCCTCGATCAACGTA
GCTACATTACCGAAGAAGACGCCCGCGGGCAGATTAACGAGCTCAAAGCTAACGGCACCGAAGCGGTGGTCGGTGCGGGG
CTGATTACCGATCTGGCAGAAGAAGCCGGAATGACCGGAATTTTTATCTATTCCGCCGTCACCGTGCGCCAGGCGTTCAG
CGATGCGCTGGATATGACGCGCATGTCGTTACGCCATAACACTCACGATGCCACCCGCAACGCCCTGAGAACTCGTTACG
TGCTGGGCGATATGCTCGGTCAATCACCACAGATGGAACAAGTACGGCAGACTATTTTGCTGTATGCCCGCTCCAGTGCG
GCGGTGTTGATTGAAGGGGAAACGGGGACGGGCAAAGAGCTGGCGGCCCAGGCGATTCATCGGGAATATTTTGCCCGCCA
CGATGCGCGACAGGGCAAAAAGTCGCATCCGTTTGTTGCCGTCAACTGCGGGGCGATAGCCGAATCGCTGCTGGAAGCGG
AGCTGTTTGGCTATGAGGAAGGGGCGTTTACCGGCTCGCGACGCGGAGGTCGCGCCGGGCTGTTCGAAATTGCCCACGGC
GGTACGCTGTTTCTGGATGAGATTGGCGAAATGCCGCTACCTTTGCAGACTCGCCTGTTACGGGTGCTGGAAGAAAAAGA
GGTTACTCGCGTCGGCGGGCATCAGCCTGTTCCGGTGGATGTGCGGGTCATTAGCGCCACTCACTGCAATCTGGAAGAAG
ATATGCGGCAAGGGCAGTTTCGCCGTGATCTGTTTTATCGGCTGAGTATTTTGCGTCTGCAATTGCCACCACTGCGCGAG
CGGGTGGCGGATATTCTTCCGCTGGCGGAAAGCTTTTTGAAAGTGTCTCTGGCGGCGCTCTCCGCCCCGTTTTCTGCCGC
ATTACGCCAGGGATTACAGGCAAGCGAAACCGTGCTGGTGCACTACGACTGGCCAGGCAATATTCGTGAACTGCGCAATA
TGATGGAACGACTGGCGCTGTTTTTAAGTGTGGAACCGACGCCGGATTTAACGCCGCAGTTTATGCAACTGCTACTGCCG
GAGCTGGCGCGCGAGTCGGCGAAAATTCCCGCTCCACGCTTACTGACACCACAACAGGCACTGGAGAAATTTAAAGGCGA
TAAAACAGCAGCGGCGAATTATTTAGGCATAAGCCGGACGACGTTCTGGCGGCGGCTGAAAAACTGA

Upstream 100 bases:

>100_bases
TTTCAGTTAACGTTTCAGGCAATGTTTCACCGCGTTTCATTGCAACAATTATGAAACAAGACTAAACCCAATATTCGGTT
TCTTAACTTTGCGGTGCGCT

Downstream 100 bases:

>100_bases
ACTGCCGAATGCAGCGCGAACGCTTTATCCGGCATAAATTTCAATAGATGATGATCACCGATAAATTTACGCATCGGGGA
TGTTTACATAGGTTTCAGAA

Product: propionate catabolism operon regulatory protein PrpR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFMQLLLP
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN

Sequences:

>Translated_528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFMQLLLP
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
>Mature_527_residues
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSV
PVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGL
ITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSAA
VLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGG
TLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRER
VADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFMQLLLPE
LARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN

Specific function: Involved in the transcriptional regulation of the propionate catabolism operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=528, Percent_Identity=98.4848484848485, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=42.603550295858, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1790299, Length=345, Percent_Identity=43.1884057971014, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1790437, Length=329, Percent_Identity=43.161094224924, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=355, Percent_Identity=42.2535211267606, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=242, Percent_Identity=44.6280991735537, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1788905, Length=349, Percent_Identity=40.1146131805158, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=37.0481927710843, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=45.5284552845528, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=43.5684647302905, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1787583, Length=237, Percent_Identity=44.3037974683544, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=33.993399339934, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.9832635983264, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR012704
- InterPro:   IPR010524 [H]

Pfam domain/function: PF02954 HTH_8; PF06506 PrpR_N; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58703; Mature: 58572

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANE
CCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCH
RCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAV
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAPFSAALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFMQLLLP
CCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANE
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCH
RCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAV
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAPFSAALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFMQLLLP
CCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]