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The map label for this gene is ecpD [H]

Identifier: 157159607

GI number: 157159607

Start: 154502

End: 155242

Strand: Reverse

Name: ecpD [H]

Synonym: EcHS_A0143

Alternate gene names: 157159607

Gene position: 155242-154502 (Counterclockwise)

Preceding gene: 157159608

Following gene: 157159606

Centisome position: 3.34

GC content: 44.26

Gene sequence:

>741_bases
ATGTTTTTTAACATCAAACATACAACAGCTTTATGCTTCGTAACCTGTATGGCTTTTAGTTCATCGTCTATTGCGGACAT
TGTCATTTCGGGTACTCGCGTAATATATAAAAGCGATCAAAAAAGTGTCAACGTACGTCTGGAAAATAAAGGGAATAACC
CGTTGCTTGTCCAGAGTTGGTTAGATACTGGCGATGACAACGCTGAGCCTGGCAGTATTACAGTCCCTTTTACTGCCACG
CCGCCAGTATCGCGTATTGATGCCAAACGTGGGCAAACAATCAAATTAATGTACACAGCCAGCACCTCACTGCCTAAAGA
CAGAGAGAGCGTGTTCTGGTTTAACGTACTGGAAGTTCCACCAAAACCAGATGCAGAAAAGGTCGCGAATCAAAGTCTGC
TGCAACTGGCATTTCGCACACGTATAAAACTTTTCTATCGCCCGGATGGATTGAAGGGAAATCCCTCTGAAGCCCCGTTA
GCCCTGAAGTGGTTCTGGTCAGGTTCAGAAGGTAAGGCGTCATTGCGAGTGACCAATCCAACCCCTTACTACGTCTCTTT
TAGCAGTGGTGATTTAGAAGCTAGCGGTAAACGCTATCCGATTGATGTGAAAATGATTGCACCATTTAGTGATGAGGTCA
TGAAAGTCACTGGCCTTAATGGCAAAGCAAGTTCTGCAAAAGTACATTTTTACGCCATTAATGATTTTGGTGGCGCCATT
GAAGGTAATGCCAGTCTGTAA

Upstream 100 bases:

>100_bases
TCACCAATTAAATTGATTGAATGAATATACAGGGAATAGTATTAATAATTTCTATTTTTATATTATTCCCTGTTTTAATT
AACTCTGTCAGGGATGGTTT

Downstream 100 bases:

>100_bases
TCAGGCAAGGATATAATTCCGCAGGAAGCATAACGTGACTATAAAACCAACTAAAAATTTGAATCGCCACGGGTTTAACA
GACACCTCAGAGTCATTTAA

Product: putative chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGAI
EGNASL

Sequences:

>Translated_246_residues
MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGAI
EGNASL
>Mature_246_residues
MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGAI
EGNASL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=98.3739837398374, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1788428, Length=244, Percent_Identity=34.4262295081967, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789532, Length=230, Percent_Identity=33.9130434782609, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788677, Length=211, Percent_Identity=36.9668246445498, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=30.1310043668122, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=31.3807531380753, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1786936, Length=210, Percent_Identity=30.4761904761905, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081806, Length=216, Percent_Identity=32.8703703703704, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787171, Length=227, Percent_Identity=28.6343612334802, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082203, Length=241, Percent_Identity=28.2157676348548, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26957; Mature: 26957

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSW
CEEEECHHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
TPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGAI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
CCCCCC
>Mature Secondary Structure
MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLENKGNNPLLVQSW
CEEEECHHHHHHHHHHHHCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
TPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGAI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]