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The map label for this gene is purR [H]

Identifier: 156978131

GI number: 156978131

Start: 2031297

End: 2032319

Strand: Reverse

Name: purR [H]

Synonym: VIBHAR_06936

Alternate gene names: 156978131

Gene position: 2032319-2031297 (Counterclockwise)

Preceding gene: 156978132

Following gene: 156978130

Centisome position: 92.21

GC content: 46.14

Gene sequence:

>1023_bases
ATGAAAAAGAAGAAAAGAGCGGCATCCATCTATGATGTTGCCAAGTTGGCAGGTGTGTCACCGAGCACCGTATCACGTTT
CCTCAATCGCACGACCTTTGTCTCTGATGATAAAAGCCAAAACATAGAAAAAGCGATCAAGTCACTGGGCTATAAGCCAA
ATTATCAAATGGGTCAGGGCACTAACACGCGTTCTATGACCATTGGCGTTCTTATCCAACACCCAGAAAGCCCGCACTCC
AGTCGTATCCTTAATGATATGGAAAAGGTTCTGAATACTCAGGGTTACTCGTTGGTGATTGCGACGGGGCATTGGCAGCG
TTCATTGGAATCGCATTCTTTGGAGTATTTGGCGAAGAGCAATGTCGATGGGGTCATCATTGTAACGGGCAGTTTGACGG
AAGCGCAGATACTGGAATATGCAGAGCGAATTCCGGTTGTTGCAGTTGGATACAATGTCATTGGCGATAAGGTTCGATCC
ATAAATATAGATAATGTATTGGGCGGATACATGGCGACACTACATTTGATGCAACAAGGTCATGTGAACATCGCTCATAT
CAAAGGTCTTTTGAATCAACCAGATGCGGTGGCTCGCTTTGTTGGTTACAAGAACGCTCTGCAAGAGGCAGGCATTAAGG
TGATGCCAAAATTAGTCAAACAAGGTGATTTCAGCAGTGAGTCTGGTTATCAAAAAACGGTGGAATTGCTCGAATCTAAA
GTGCATTTCTCTGCATTGTTTGCTGCCAATGACCAAACGGCTTATGGGGCGATCAAGGCTCTGCATGACCACGACGTTCG
TGTGCCAGAGGATGTGTCTGTGATCGGCTTTGATGACTTGCCGACCTCACAATATTTCACGCCAGCACTTACGACACTGA
GACAGCCGATTGAAGAAGTTGGAGCCATTTGTGCCGAGTCCATACTCAATTTATTGAGTGGTCAAAGGCATGAAGTGCGT
CTACCTCCGATCGATCTGATTGTCCGCCAATCAACGGTGTCTAAGTTCCGCCAATCAAGTTGA

Upstream 100 bases:

>100_bases
CTGGCGACTTATCTAGCTCTCATTTCCCATATCAAAATGTTGTCGCGTCTCCCGCTGCAACTTATATTTTTGTTATTAGC
TTGCTGGGGAAATTCCTACA

Downstream 100 bases:

>100_bases
GATAAGTCGCCAAACCCATTAGAAAATATTTTCCGAAACTCGACGAAATCTTGTTGTATTTGTGACCAAACTCCGTCTAG
AATTCGCGCCTGCATTTTGG

Product: hypothetical protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKKKRAASIYDVAKLAGVSPSTVSRFLNRTTFVSDDKSQNIEKAIKSLGYKPNYQMGQGTNTRSMTIGVLIQHPESPHS
SRILNDMEKVLNTQGYSLVIATGHWQRSLESHSLEYLAKSNVDGVIIVTGSLTEAQILEYAERIPVVAVGYNVIGDKVRS
INIDNVLGGYMATLHLMQQGHVNIAHIKGLLNQPDAVARFVGYKNALQEAGIKVMPKLVKQGDFSSESGYQKTVELLESK
VHFSALFAANDQTAYGAIKALHDHDVRVPEDVSVIGFDDLPTSQYFTPALTTLRQPIEEVGAICAESILNLLSGQRHEVR
LPPIDLIVRQSTVSKFRQSS

Sequences:

>Translated_340_residues
MKKKKRAASIYDVAKLAGVSPSTVSRFLNRTTFVSDDKSQNIEKAIKSLGYKPNYQMGQGTNTRSMTIGVLIQHPESPHS
SRILNDMEKVLNTQGYSLVIATGHWQRSLESHSLEYLAKSNVDGVIIVTGSLTEAQILEYAERIPVVAVGYNVIGDKVRS
INIDNVLGGYMATLHLMQQGHVNIAHIKGLLNQPDAVARFVGYKNALQEAGIKVMPKLVKQGDFSSESGYQKTVELLESK
VHFSALFAANDQTAYGAIKALHDHDVRVPEDVSVIGFDDLPTSQYFTPALTTLRQPIEEVGAICAESILNLLSGQRHEVR
LPPIDLIVRQSTVSKFRQSS
>Mature_340_residues
MKKKKRAASIYDVAKLAGVSPSTVSRFLNRTTFVSDDKSQNIEKAIKSLGYKPNYQMGQGTNTRSMTIGVLIQHPESPHS
SRILNDMEKVLNTQGYSLVIATGHWQRSLESHSLEYLAKSNVDGVIIVTGSLTEAQILEYAERIPVVAVGYNVIGDKVRS
INIDNVLGGYMATLHLMQQGHVNIAHIKGLLNQPDAVARFVGYKNALQEAGIKVMPKLVKQGDFSSESGYQKTVELLESK
VHFSALFAANDQTAYGAIKALHDHDVRVPEDVSVIGFDDLPTSQYFTPALTTLRQPIEEVGAICAESILNLLSGQRHEVR
LPPIDLIVRQSTVSKFRQSS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=314, Percent_Identity=33.4394904458599, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1790194, Length=322, Percent_Identity=31.6770186335404, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789068, Length=296, Percent_Identity=31.7567567567568, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=32.6460481099656, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=31.2703583061889, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=32.2388059701493, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=30.5232558139535, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=27.4447949526814, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=28.6153846153846, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=25.4716981132075, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787906, Length=300, Percent_Identity=25.6666666666667, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=315, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=22.4683544303797, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=21.3375796178344, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790192, Length=277, Percent_Identity=24.187725631769, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37402; Mature: 37402

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKRAASIYDVAKLAGVSPSTVSRFLNRTTFVSDDKSQNIEKAIKSLGYKPNYQMGQG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TNTRSMTIGVLIQHPESPHSSRILNDMEKVLNTQGYSLVIATGHWQRSLESHSLEYLAKS
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHC
NVDGVIIVTGSLTEAQILEYAERIPVVAVGYNVIGDKVRSINIDNVLGGYMATLHLMQQG
CCCEEEEEECCCHHHHHHHHHHHCCEEEECHHHHCCHHHCCCHHHHHHHHHHHHHHHHCC
HVNIAHIKGLLNQPDAVARFVGYKNALQEAGIKVMPKLVKQGDFSSESGYQKTVELLESK
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VHFSALFAANDQTAYGAIKALHDHDVRVPEDVSVIGFDDLPTSQYFTPALTTLRQPIEEV
HHHHHEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
GAICAESILNLLSGQRHEVRLPPIDLIVRQSTVSKFRQSS
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKKRAASIYDVAKLAGVSPSTVSRFLNRTTFVSDDKSQNIEKAIKSLGYKPNYQMGQG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TNTRSMTIGVLIQHPESPHSSRILNDMEKVLNTQGYSLVIATGHWQRSLESHSLEYLAKS
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHC
NVDGVIIVTGSLTEAQILEYAERIPVVAVGYNVIGDKVRSINIDNVLGGYMATLHLMQQG
CCCEEEEEECCCHHHHHHHHHHHCCEEEECHHHHCCHHHCCCHHHHHHHHHHHHHHHHCC
HVNIAHIKGLLNQPDAVARFVGYKNALQEAGIKVMPKLVKQGDFSSESGYQKTVELLESK
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VHFSALFAANDQTAYGAIKALHDHDVRVPEDVSVIGFDDLPTSQYFTPALTTLRQPIEEV
HHHHHEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHH
GAICAESILNLLSGQRHEVRLPPIDLIVRQSTVSKFRQSS
HHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA