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The map label for this gene is dctB [H]

Identifier: 156977928

GI number: 156977928

Start: 1855957

End: 1858017

Strand: Direct

Name: dctB [H]

Synonym: VIBHAR_06723

Alternate gene names: 156977928

Gene position: 1855957-1858017 (Clockwise)

Preceding gene: 156977926

Following gene: 156977929

Centisome position: 84.21

GC content: 46.43

Gene sequence:

>2061_bases
ATGCAGCGTAGAAGAATTGGCAAAACATGGCTTGTAGTCGCACTTGCATCCATGCTGATTTTGTCTTGCTATATCGCCAG
TCGAGAGGTTGCTCGTCAATGGTTGAGTGTCGAGACTCAAAACGAAGCTCAACAAAAGTTGCTCGACTATATCGGTGAAG
TTCGCCGGGCTCTCAAACGCTTCTATCACCTGCCATACCTTGTTACCAATAACCCGGAAACCATCGCGTTTGTAAAAGGG
AATCTAACTTATTACGAGCCGCTACAAACCCAACTGACTCAACTGGATAAAGCCGCTAACACAGAAGGTTGGCTAATCCT
GTCTAACTCTGGTGAGGTACTGGTGTCGAGTTTAATCGAAGAGCGCTTTAGCCTGTCTGACCGAAAAGCGATTGTGGAAC
AAATTCACAATCAAGGCGGCGGTGTCTCCTTGGTGAACAAAACCAAAGGCTCCAGCCCACTGTATTATTTGGCCGCTCCT
ATCTATGACGGCTTGGATATCGCTGGCATTGTGGTAGTACAAATCAACCTGAGCCTATTGACTGAGCAAACCATTACCAG
TCAAGACATCATCACATTGCAAAATCGCCAGCAGCGTTTCTTCCTATCGAGCAGCCCAGTCTATAACGCAGACTGGTTTA
ACGAACCGAACAACACAGTCCAGCTAGAAACTCAACGCTTATACGACAACACCAATATCAGAGCATGGACACTCAACCAT
CAACGCTATTTTGCACATACCGTGAAGCTGGATGACTTGAATTGGTACGTCAGTTACCTCACCCCTTCCAAACCGATTCA
ACAAACTATCAATGTGATTGGTTGGAGCGTAGCGGCACTGCTCCTATTGATCGTCTTGCTTGTCATCATTGGCTTGCAAC
GTCGCCAAAAACACATCAACCAACAACGGATTCAAGCCCTTTTGGAAGAGTCCCAACGCCGCTTGAAGCAAATGATCAAT
AAGACTCACGTTGGTTTGTTATTGATTGACGAACAAGGGCAACTGTTTGACCTCAACCCGATGGCGAAGCGTTTGTTTTG
TTTGCCAGAGAGTGTTAATCGACACACTTTTGCTTGGGAGCTATTTGATGCCGGTAACCCAAACTCCACAACTCTCAAGT
TACTTAAAGATCTACCTAAACATAAAGAGCTCGCAGAGATCAGCACGGTCGAAACCATCGCCAAAAGGAGTGATGGCAGC
GTCTTCCCTGTTCTGTTCTCCCTGACCAGTTTCCCTTGGCATGGCGAGCAGTATTTCCTTGCAACGATCATAGACATCAG
TAAGCGGAAAAAAGCCGAGCAATCACTGCAACAGATTAACCAAGAACTGGCGCAACGGGTAGAAGAGCGAACTCAAGCGC
TGAAAGAAGCACAAAGCCAGTTAATTGAATCGAGCAAAATGGCAGCCTTAGGTCGTATGTCGAGCGCGATCACTCATGAA
CTTAATCAGCCGCTAACGGGCTTGCGTACTCTGCTCACCACTAATGAGCTATTGGCTGAACGCGGTGAAACCGACATGAT
GAAAGCCAATAACGTCTTGGTACAGAAACTGATTGATCGCATGGCAAGCATGACCAGTCAGCTAAAAACGTTCGCTTACA
ACAAACCACAACGTTTACAAGCGATTTCCCTTACCTCTGCACTCGAAGAAACATTGCGTGTTTACCAATCTCAACTAGAC
AAGGTGGATGTACGGGTCCGTGTTCCTCGAAATTTAAACAAGATCATGGGAGAAGAACAACGACTGCTGCAGGTGCTTGG
CAACCTTGTATCGAACGCATTGGATGCGATGGGCAACACCACTAATCCTCAGCTCAACTTGATCGTCAATCCAACGGCAA
ATCAGGTAGAACTAACGGTGAGTGACAATGGCTGCGGCGTCTCAGATGAGATGCTCGAATCGATGTTTGAACCCTTCCAA
ACCTCAAAGAAAATTGGTGAGGGGCTAGGGCTGGGCTTAGCGATTACGGCCAACAATATGCGTGATATGAATGGACAAGT
TACCGTGCATAAGAATGAACAAAATGGACTGACTTTCACTTTATTGTTCAAATCAGCTTAA

Upstream 100 bases:

>100_bases
AGGAGCTAGATCTCACTTCTTGCTTACAAGTCTTTAACATCCAACCAGTTAGGCGCACTCTTTCATAGTCGAAAAAGATC
AATACCAGTATGGGGGCGGT

Downstream 100 bases:

>100_bases
GTCGATATTGATTACAAAATCATCATCAGAACTCGGTATCTCCCACCGTTTGAGAAAAACACGATCTCGATACAAGAATC
CCACCTTGACCTACACTATT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQRRRIGKTWLVVALASMLILSCYIASREVARQWLSVETQNEAQQKLLDYIGEVRRALKRFYHLPYLVTNNPETIAFVKG
NLTYYEPLQTQLTQLDKAANTEGWLILSNSGEVLVSSLIEERFSLSDRKAIVEQIHNQGGGVSLVNKTKGSSPLYYLAAP
IYDGLDIAGIVVVQINLSLLTEQTITSQDIITLQNRQQRFFLSSSPVYNADWFNEPNNTVQLETQRLYDNTNIRAWTLNH
QRYFAHTVKLDDLNWYVSYLTPSKPIQQTINVIGWSVAALLLLIVLLVIIGLQRRQKHINQQRIQALLEESQRRLKQMIN
KTHVGLLLIDEQGQLFDLNPMAKRLFCLPESVNRHTFAWELFDAGNPNSTTLKLLKDLPKHKELAEISTVETIAKRSDGS
VFPVLFSLTSFPWHGEQYFLATIIDISKRKKAEQSLQQINQELAQRVEERTQALKEAQSQLIESSKMAALGRMSSAITHE
LNQPLTGLRTLLTTNELLAERGETDMMKANNVLVQKLIDRMASMTSQLKTFAYNKPQRLQAISLTSALEETLRVYQSQLD
KVDVRVRVPRNLNKIMGEEQRLLQVLGNLVSNALDAMGNTTNPQLNLIVNPTANQVELTVSDNGCGVSDEMLESMFEPFQ
TSKKIGEGLGLGLAITANNMRDMNGQVTVHKNEQNGLTFTLLFKSA

Sequences:

>Translated_686_residues
MQRRRIGKTWLVVALASMLILSCYIASREVARQWLSVETQNEAQQKLLDYIGEVRRALKRFYHLPYLVTNNPETIAFVKG
NLTYYEPLQTQLTQLDKAANTEGWLILSNSGEVLVSSLIEERFSLSDRKAIVEQIHNQGGGVSLVNKTKGSSPLYYLAAP
IYDGLDIAGIVVVQINLSLLTEQTITSQDIITLQNRQQRFFLSSSPVYNADWFNEPNNTVQLETQRLYDNTNIRAWTLNH
QRYFAHTVKLDDLNWYVSYLTPSKPIQQTINVIGWSVAALLLLIVLLVIIGLQRRQKHINQQRIQALLEESQRRLKQMIN
KTHVGLLLIDEQGQLFDLNPMAKRLFCLPESVNRHTFAWELFDAGNPNSTTLKLLKDLPKHKELAEISTVETIAKRSDGS
VFPVLFSLTSFPWHGEQYFLATIIDISKRKKAEQSLQQINQELAQRVEERTQALKEAQSQLIESSKMAALGRMSSAITHE
LNQPLTGLRTLLTTNELLAERGETDMMKANNVLVQKLIDRMASMTSQLKTFAYNKPQRLQAISLTSALEETLRVYQSQLD
KVDVRVRVPRNLNKIMGEEQRLLQVLGNLVSNALDAMGNTTNPQLNLIVNPTANQVELTVSDNGCGVSDEMLESMFEPFQ
TSKKIGEGLGLGLAITANNMRDMNGQVTVHKNEQNGLTFTLLFKSA
>Mature_686_residues
MQRRRIGKTWLVVALASMLILSCYIASREVARQWLSVETQNEAQQKLLDYIGEVRRALKRFYHLPYLVTNNPETIAFVKG
NLTYYEPLQTQLTQLDKAANTEGWLILSNSGEVLVSSLIEERFSLSDRKAIVEQIHNQGGGVSLVNKTKGSSPLYYLAAP
IYDGLDIAGIVVVQINLSLLTEQTITSQDIITLQNRQQRFFLSSSPVYNADWFNEPNNTVQLETQRLYDNTNIRAWTLNH
QRYFAHTVKLDDLNWYVSYLTPSKPIQQTINVIGWSVAALLLLIVLLVIIGLQRRQKHINQQRIQALLEESQRRLKQMIN
KTHVGLLLIDEQGQLFDLNPMAKRLFCLPESVNRHTFAWELFDAGNPNSTTLKLLKDLPKHKELAEISTVETIAKRSDGS
VFPVLFSLTSFPWHGEQYFLATIIDISKRKKAEQSLQQINQELAQRVEERTQALKEAQSQLIESSKMAALGRMSSAITHE
LNQPLTGLRTLLTTNELLAERGETDMMKANNVLVQKLIDRMASMTSQLKTFAYNKPQRLQAISLTSALEETLRVYQSQLD
KVDVRVRVPRNLNKIMGEEQRLLQVLGNLVSNALDAMGNTTNPQLNLIVNPTANQVELTVSDNGCGVSDEMLESMFEPFQ
TSKKIGEGLGLGLAITANNMRDMNGQVTVHKNEQNGLTFTLLFKSA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=28.2608695652174, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=27.8969957081545, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081816, Length=272, Percent_Identity=26.8382352941176, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=286, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 77623; Mature: 77623

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRRIGKTWLVVALASMLILSCYIASREVARQWLSVETQNEAQQKLLDYIGEVRRALKR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FYHLPYLVTNNPETIAFVKGNLTYYEPLQTQLTQLDKAANTEGWLILSNSGEVLVSSLIE
HHCCCEEEECCCCEEEEEECCEEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
ERFSLSDRKAIVEQIHNQGGGVSLVNKTKGSSPLYYLAAPIYDGLDIAGIVVVQINLSLL
HHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEHHHH
TEQTITSQDIITLQNRQQRFFLSSSPVYNADWFNEPNNTVQLETQRLYDNTNIRAWTLNH
HHHHCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECC
QRYFAHTVKLDDLNWYVSYLTPSKPIQQTINVIGWSVAALLLLIVLLVIIGLQRRQKHIN
CEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
QQRIQALLEESQRRLKQMINKTHVGLLLIDEQGQLFDLNPMAKRLFCLPESVNRHTFAWE
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHCCCCCCCCCCEEEEE
LFDAGNPNSTTLKLLKDLPKHKELAEISTVETIAKRSDGSVFPVLFSLTSFPWHGEQYFL
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEH
ATIIDISKRKKAEQSLQQINQELAQRVEERTQALKEAQSQLIESSKMAALGRMSSAITHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNQPLTGLRTLLTTNELLAERGETDMMKANNVLVQKLIDRMASMTSQLKTFAYNKPQRLQ
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISLTSALEETLRVYQSQLDKVDVRVRVPRNLNKIMGEEQRLLQVLGNLVSNALDAMGNT
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
TNPQLNLIVNPTANQVELTVSDNGCGVSDEMLESMFEPFQTSKKIGEGLGLGLAITANNM
CCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
RDMNGQVTVHKNEQNGLTFTLLFKSA
CCCCCEEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MQRRRIGKTWLVVALASMLILSCYIASREVARQWLSVETQNEAQQKLLDYIGEVRRALKR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FYHLPYLVTNNPETIAFVKGNLTYYEPLQTQLTQLDKAANTEGWLILSNSGEVLVSSLIE
HHCCCEEEECCCCEEEEEECCEEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
ERFSLSDRKAIVEQIHNQGGGVSLVNKTKGSSPLYYLAAPIYDGLDIAGIVVVQINLSLL
HHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEHHHH
TEQTITSQDIITLQNRQQRFFLSSSPVYNADWFNEPNNTVQLETQRLYDNTNIRAWTLNH
HHHHCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECC
QRYFAHTVKLDDLNWYVSYLTPSKPIQQTINVIGWSVAALLLLIVLLVIIGLQRRQKHIN
CEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
QQRIQALLEESQRRLKQMINKTHVGLLLIDEQGQLFDLNPMAKRLFCLPESVNRHTFAWE
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHCCCCCCCCCCEEEEE
LFDAGNPNSTTLKLLKDLPKHKELAEISTVETIAKRSDGSVFPVLFSLTSFPWHGEQYFL
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEH
ATIIDISKRKKAEQSLQQINQELAQRVEERTQALKEAQSQLIESSKMAALGRMSSAITHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNQPLTGLRTLLTTNELLAERGETDMMKANNVLVQKLIDRMASMTSQLKTFAYNKPQRLQ
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISLTSALEETLRVYQSQLDKVDVRVRVPRNLNKIMGEEQRLLQVLGNLVSNALDAMGNT
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
TNPQLNLIVNPTANQVELTVSDNGCGVSDEMLESMFEPFQTSKKIGEGLGLGLAITANNM
CCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
RDMNGQVTVHKNEQNGLTFTLLFKSA
CCCCCEEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]