Click here to switch to the map view.

The map label for this gene is brnQ [H]

Identifier: 156977926

GI number: 156977926

Start: 1853056

End: 1854366

Strand: Direct

Name: brnQ [H]

Synonym: VIBHAR_06721

Alternate gene names: 156977926

Gene position: 1853056-1854366 (Clockwise)

Preceding gene: 156977923

Following gene: 156977928

Centisome position: 84.08

GC content: 49.2

Gene sequence:

>1311_bases
ATGTTAAGCGCACGCAATATCGCCGCTTTGGGCTTCATGACTTTCGCCATGTACTTAGGCGCGGGTAACCTGATCTTCCC
TCCATTTTTGGGTTATCAAGCAGGTGAAAACTTTCTGAGCGGGATGTCTGGTTTCCTCTTGACGGGCGTAGGTCTGCCTG
CAATGGCACTAGTAATGGTGGTTATCGTTAACGGTTCAGACAAACTAACCGCTGCCCTACCTAAACCTTTGGCAACCAGT
TTTTGGGTGATGGTGTTTATCGTGATTGGCCCTGCGTTTGTGATTCCACGCGCGATTACTGTGGCTTACCAATTTAGCTT
TGCACCAATGTTTGGTGATGCAGCTTTGGTGCCTTTCACTGTTGCCTTCTGTGTTATCACCATTTTATTCGCCCTATATC
CGGGTAAGTTGGTCGACAACCTAGGCAAGATCTTAACTCCCGCTTTGATGGCTATCTTGGTGAGCATGTCTGTGACCGCT
CTGGTTTATCCTGCTGGCGAACTAACCACAGCAACGGGTCCATACGTATCTGGCGCGTTTGCTGAAGGCTTAACCCAAGG
CTACATGACCATGGATGCGCTAGGCTCTATAGGTTTTGGTTGGATTATTTTCCGTGCGATTCGCAGCATGGGGGTAGATT
GTCCGAAAGCCACCGCAAAATACACGCTAATCGCCGCTTTGATGTACGCGGTTGCCATGGCATTTGTTTACATCTCATTG
GCTTACATAGGCTCGACGAGCTCATACTTAGGTTCTGAATTCAGTAACGGTGGCGATATCCTAACGGCGTTCACCTTCAA
CCACTTTGGTGCGTTTGGTTCACTATTGCTAGGTGCCGTAATGGTATTGGCTTGTTTGACTACTGCTATCGGCGTAACCA
CAGCAGGTAGTGAGTTTTACGACAACACTTTCAGCAAAGTGAACTACAAAGGCTGCGTTATTACGACCATGGTGTTGGCA
GGTTTGATTGCTAACGTAGGTCTGGAGCAACTATTGGCTATCACATTGCCTGCGGTTGTGGTGCTTCACCCAGTGGCGAT
TGCATTGATGATCATGGCACCAGTGCGCAACAAGATGTCGCAAAGCATGGTCGTCGCGACGGCGTTCACTGCGCTAACGT
TTGGTTGTGTTGATGCGCTGCACATCCTAGGTTACATGCCAGAAGCGGCAAACCAATGGTTAGACAGCAACATGCCACTG
TACCACTACTTTGCAAGTTGGATTGTTCCGACGATCGCTATGGTGGTTGTGGGCGTAGTATTTACCCGCAAGGCAAGCCA
ACAACGCACCGAATTCGTGAACCAAGCCTAG

Upstream 100 bases:

>100_bases
ATATAAACTTTCTGTCAGCTTGAGAAGAAGCTGACGGGAACACCACATACTCCTCCCATTCCTTTCAGTTTCTTCCAAGC
GAAACATAAGAGGAATAACA

Downstream 100 bases:

>100_bases
ATTGGCAAGTACCTTAACAAATAAGTACCTCAACAAAAAACGGCTTCCTAAGTGAAGCCGTTTTTTCTACGTGTAATCAG
AGCGATTCAACCCAAACTTC

Product: hypothetical protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLSARNIAALGFMTFAMYLGAGNLIFPPFLGYQAGENFLSGMSGFLLTGVGLPAMALVMVVIVNGSDKLTAALPKPLATS
FWVMVFIVIGPAFVIPRAITVAYQFSFAPMFGDAALVPFTVAFCVITILFALYPGKLVDNLGKILTPALMAILVSMSVTA
LVYPAGELTTATGPYVSGAFAEGLTQGYMTMDALGSIGFGWIIFRAIRSMGVDCPKATAKYTLIAALMYAVAMAFVYISL
AYIGSTSSYLGSEFSNGGDILTAFTFNHFGAFGSLLLGAVMVLACLTTAIGVTTAGSEFYDNTFSKVNYKGCVITTMVLA
GLIANVGLEQLLAITLPAVVVLHPVAIALMIMAPVRNKMSQSMVVATAFTALTFGCVDALHILGYMPEAANQWLDSNMPL
YHYFASWIVPTIAMVVVGVVFTRKASQQRTEFVNQA

Sequences:

>Translated_436_residues
MLSARNIAALGFMTFAMYLGAGNLIFPPFLGYQAGENFLSGMSGFLLTGVGLPAMALVMVVIVNGSDKLTAALPKPLATS
FWVMVFIVIGPAFVIPRAITVAYQFSFAPMFGDAALVPFTVAFCVITILFALYPGKLVDNLGKILTPALMAILVSMSVTA
LVYPAGELTTATGPYVSGAFAEGLTQGYMTMDALGSIGFGWIIFRAIRSMGVDCPKATAKYTLIAALMYAVAMAFVYISL
AYIGSTSSYLGSEFSNGGDILTAFTFNHFGAFGSLLLGAVMVLACLTTAIGVTTAGSEFYDNTFSKVNYKGCVITTMVLA
GLIANVGLEQLLAITLPAVVVLHPVAIALMIMAPVRNKMSQSMVVATAFTALTFGCVDALHILGYMPEAANQWLDSNMPL
YHYFASWIVPTIAMVVVGVVFTRKASQQRTEFVNQA
>Mature_436_residues
MLSARNIAALGFMTFAMYLGAGNLIFPPFLGYQAGENFLSGMSGFLLTGVGLPAMALVMVVIVNGSDKLTAALPKPLATS
FWVMVFIVIGPAFVIPRAITVAYQFSFAPMFGDAALVPFTVAFCVITILFALYPGKLVDNLGKILTPALMAILVSMSVTA
LVYPAGELTTATGPYVSGAFAEGLTQGYMTMDALGSIGFGWIIFRAIRSMGVDCPKATAKYTLIAALMYAVAMAFVYISL
AYIGSTSSYLGSEFSNGGDILTAFTFNHFGAFGSLLLGAVMVLACLTTAIGVTTAGSEFYDNTFSKVNYKGCVITTMVLA
GLIANVGLEQLLAITLPAVVVLHPVAIALMIMAPVRNKMSQSMVVATAFTALTFGCVDALHILGYMPEAANQWLDSNMPL
YHYFASWIVPTIAMVVVGVVFTRKASQQRTEFVNQA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=438, Percent_Identity=37.4429223744292, Blast_Score=284, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46187; Mature: 46187

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSARNIAALGFMTFAMYLGAGNLIFPPFLGYQAGENFLSGMSGFLLTGVGLPAMALVMV
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIVNGSDKLTAALPKPLATSFWVMVFIVIGPAFVIPRAITVAYQFSFAPMFGDAALVPFT
HHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
VAFCVITILFALYPGKLVDNLGKILTPALMAILVSMSVTALVYPAGELTTATGPYVSGAF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHH
AEGLTQGYMTMDALGSIGFGWIIFRAIRSMGVDCPKATAKYTLIAALMYAVAMAFVYISL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYIGSTSSYLGSEFSNGGDILTAFTFNHFGAFGSLLLGAVMVLACLTTAIGVTTAGSEFY
HHHCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
DNTFSKVNYKGCVITTMVLAGLIANVGLEQLLAITLPAVVVLHPVAIALMIMAPVRNKMS
HCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSMVVATAFTALTFGCVDALHILGYMPEAANQWLDSNMPLYHYFASWIVPTIAMVVVGVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FTRKASQQRTEFVNQA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSARNIAALGFMTFAMYLGAGNLIFPPFLGYQAGENFLSGMSGFLLTGVGLPAMALVMV
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIVNGSDKLTAALPKPLATSFWVMVFIVIGPAFVIPRAITVAYQFSFAPMFGDAALVPFT
HHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
VAFCVITILFALYPGKLVDNLGKILTPALMAILVSMSVTALVYPAGELTTATGPYVSGAF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHH
AEGLTQGYMTMDALGSIGFGWIIFRAIRSMGVDCPKATAKYTLIAALMYAVAMAFVYISL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYIGSTSSYLGSEFSNGGDILTAFTFNHFGAFGSLLLGAVMVLACLTTAIGVTTAGSEFY
HHHCCCHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
DNTFSKVNYKGCVITTMVLAGLIANVGLEQLLAITLPAVVVLHPVAIALMIMAPVRNKMS
HCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSMVVATAFTALTFGCVDALHILGYMPEAANQWLDSNMPLYHYFASWIVPTIAMVVVGVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FTRKASQQRTEFVNQA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]