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The map label for this gene is tnaA [H]

Identifier: 156977914

GI number: 156977914

Start: 1843523

End: 1844956

Strand: Reverse

Name: tnaA [H]

Synonym: VIBHAR_06709

Alternate gene names: 156977914

Gene position: 1844956-1843523 (Counterclockwise)

Preceding gene: 156977916

Following gene: 156977912

Centisome position: 83.71

GC content: 48.19

Gene sequence:

>1434_bases
ATGCGTAGGGTCATGCGTATAAGGAAATTAACAATGAAACGTATCCCAGAACCGTTCCGTATCAAAATGGTTGAGCCAAT
CAAAATGACGACCTTGGCTGACCGAGACGTTGCCCTTAAACAAGCGGGCTTTAACCCATTTCTTCTTCGTAGTGATGATG
TCTACATCGACCTACTGACCGACTCTGGTACAGGTGCTATGAGTGACAATCAGTGGGCAGGTATCATGATGGGCGATGAG
TCCTATGCAGGCAGTCGTAACTATTACAACTTGTGTGACGCGGTAGAGCATTTCTTTGGTTACAAGCTCACAGTGCCTGC
GCACCAAGGTCGCGGGGCGGAGCAAATCCTATTCCCTGCTTTGATTGAACGTATGCGTCAGGTTCGCGGTGGTGAAACGC
CCGTATTTATCTCGAATTATCACTTCGATACCACTGCGGGCCACATTGAACTCAATGGCGGTAAAGCGATTAACGTAGTG
ACGGAAGAAGCGTTTGATACCACCACGCCATACGACTGGAAGGGGAACTTTGACCTTAATAAGTTGGTGGCAACCATTGA
AGAACATGGTTCTCATAACATCTGCGCGATCATCACAACGGTCACGTGTAACAGCTCTGGTGGTCAGCCTGTGTCGATGG
AAAACATGCGCAGCGTGTACGAAATCGCAACCAAGTACGACATCCCTGTGGTGATCGATTCGGCTCGCTATTGCGAGAAC
GCTTACTTTATCAAGCAGCGTGAAGCGGGTTATGAGAACAAATCGATTCTCGAAATCATTCGCGAGATGTACCAATACGG
TGACATGCTCACCATGTCAGCGAAAAAAGATCCGATGGTGAATATTGGTGGTATGTGTTGTATTCGCGATCATCAAGAGC
TTTTCCAAGCGGTTCAAACACGATGTGTACCGATGGAAGGCTTTGTCACTTACGGTGGTATGGCAGGTCGTGATATGGAA
GCCTTGGCTCGCGGATTGTATGAAGGTGCGGATGAAGATTACCTTCACTACCGCATTAGCCAAGTGCAATACTTGGGTGA
GCGTCTACGCGAGGGTGGCATTCCAATTCAATACCCAACAGGTGGTCATGCCGTCTTTGTTGATGCGGCTAAGATTCTGC
CACATATCCCACCAGAGCAATTCCCTGCACAAGTGTTATGTAATGCTTTGTACTTAGAGGCGGGGATACGAGCGGTAGAG
ATTGGGTCACTGTTGCTTGGCCGCGATCCGGAAACCGGTGAACAAAAAGCTTCAGAACTTGAGTTGATGCGTTTGACCAT
TCCACGTCGTGTGTACACCAACGATCACATGGATTACATCGCTGATGCGATCATCGAGTTGAAGAATCGTGCTCATGAAC
TGAAAGGATTGACGTTTGAATACGAACCACCAGTACTTCGTCACTTCACAGCTCGTTTCCGCGAACTGGATTAA

Upstream 100 bases:

>100_bases
TACTGCTTGTCTCTTCTTAGTAAAATCTCTGCGCGTTAATTTCCTCAATGCTCCCGCATTGAAAAGCAATTTATCAGTTT
GTTTTTAATCTTACGCTTAA

Downstream 100 bases:

>100_bases
TAATTCTGTGGGTCGTTAATGACATTGATAAACGTTAATTAAGCAAAAAGCCGCGAATTTCGCGGCTTTTTTTGATTCAT
CTGAGTGTCGTATTACGCCA

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRVMRIRKLTMKRIPEPFRIKMVEPIKMTTLADRDVALKQAGFNPFLLRSDDVYIDLLTDSGTGAMSDNQWAGIMMGDE
SYAGSRNYYNLCDAVEHFFGYKLTVPAHQGRGAEQILFPALIERMRQVRGGETPVFISNYHFDTTAGHIELNGGKAINVV
TEEAFDTTTPYDWKGNFDLNKLVATIEEHGSHNICAIITTVTCNSSGGQPVSMENMRSVYEIATKYDIPVVIDSARYCEN
AYFIKQREAGYENKSILEIIREMYQYGDMLTMSAKKDPMVNIGGMCCIRDHQELFQAVQTRCVPMEGFVTYGGMAGRDME
ALARGLYEGADEDYLHYRISQVQYLGERLREGGIPIQYPTGGHAVFVDAAKILPHIPPEQFPAQVLCNALYLEAGIRAVE
IGSLLLGRDPETGEQKASELELMRLTIPRRVYTNDHMDYIADAIIELKNRAHELKGLTFEYEPPVLRHFTARFRELD

Sequences:

>Translated_477_residues
MRRVMRIRKLTMKRIPEPFRIKMVEPIKMTTLADRDVALKQAGFNPFLLRSDDVYIDLLTDSGTGAMSDNQWAGIMMGDE
SYAGSRNYYNLCDAVEHFFGYKLTVPAHQGRGAEQILFPALIERMRQVRGGETPVFISNYHFDTTAGHIELNGGKAINVV
TEEAFDTTTPYDWKGNFDLNKLVATIEEHGSHNICAIITTVTCNSSGGQPVSMENMRSVYEIATKYDIPVVIDSARYCEN
AYFIKQREAGYENKSILEIIREMYQYGDMLTMSAKKDPMVNIGGMCCIRDHQELFQAVQTRCVPMEGFVTYGGMAGRDME
ALARGLYEGADEDYLHYRISQVQYLGERLREGGIPIQYPTGGHAVFVDAAKILPHIPPEQFPAQVLCNALYLEAGIRAVE
IGSLLLGRDPETGEQKASELELMRLTIPRRVYTNDHMDYIADAIIELKNRAHELKGLTFEYEPPVLRHFTARFRELD
>Mature_477_residues
MRRVMRIRKLTMKRIPEPFRIKMVEPIKMTTLADRDVALKQAGFNPFLLRSDDVYIDLLTDSGTGAMSDNQWAGIMMGDE
SYAGSRNYYNLCDAVEHFFGYKLTVPAHQGRGAEQILFPALIERMRQVRGGETPVFISNYHFDTTAGHIELNGGKAINVV
TEEAFDTTTPYDWKGNFDLNKLVATIEEHGSHNICAIITTVTCNSSGGQPVSMENMRSVYEIATKYDIPVVIDSARYCEN
AYFIKQREAGYENKSILEIIREMYQYGDMLTMSAKKDPMVNIGGMCCIRDHQELFQAVQTRCVPMEGFVTYGGMAGRDME
ALARGLYEGADEDYLHYRISQVQYLGERLREGGIPIQYPTGGHAVFVDAAKILPHIPPEQFPAQVLCNALYLEAGIRAVE
IGSLLLGRDPETGEQKASELELMRLTIPRRVYTNDHMDYIADAIIELKNRAHELKGLTFEYEPPVLRHFTARFRELD

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=469, Percent_Identity=56.7164179104478, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 53996; Mature: 53996

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVMRIRKLTMKRIPEPFRIKMVEPIKMTTLADRDVALKQAGFNPFLLRSDDVYIDLLT
CCHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCHHHHHCCCCCEEEECCCEEEEEEE
DSGTGAMSDNQWAGIMMGDESYAGSRNYYNLCDAVEHFFGYKLTVPAHQGRGAEQILFPA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHH
LIERMRQVRGGETPVFISNYHFDTTAGHIELNGGKAINVVTEEAFDTTTPYDWKGNFDLN
HHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHH
KLVATIEEHGSHNICAIITTVTCNSSGGQPVSMENMRSVYEIATKYDIPVVIDSARYCEN
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHCC
AYFIKQREAGYENKSILEIIREMYQYGDMLTMSAKKDPMVNIGGMCCIRDHQELFQAVQT
EEEEEEHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEECCCEEEEHHHHHHHHHHHH
RCVPMEGFVTYGGMAGRDMEALARGLYEGADEDYLHYRISQVQYLGERLREGGIPIQYPT
HCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECC
GGHAVFVDAAKILPHIPPEQFPAQVLCNALYLEAGIRAVEIGSLLLGRDPETGEQKASEL
CCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHCCCCCCCHHHHHHHH
ELMRLTIPRRVYTNDHMDYIADAIIELKNRAHELKGLTFEYEPPVLRHFTARFRELD
HHHEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRVMRIRKLTMKRIPEPFRIKMVEPIKMTTLADRDVALKQAGFNPFLLRSDDVYIDLLT
CCHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCHHHHHCCCCCEEEECCCEEEEEEE
DSGTGAMSDNQWAGIMMGDESYAGSRNYYNLCDAVEHFFGYKLTVPAHQGRGAEQILFPA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHH
LIERMRQVRGGETPVFISNYHFDTTAGHIELNGGKAINVVTEEAFDTTTPYDWKGNFDLN
HHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHH
KLVATIEEHGSHNICAIITTVTCNSSGGQPVSMENMRSVYEIATKYDIPVVIDSARYCEN
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHCC
AYFIKQREAGYENKSILEIIREMYQYGDMLTMSAKKDPMVNIGGMCCIRDHQELFQAVQT
EEEEEEHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEECCCEEEEHHHHHHHHHHHH
RCVPMEGFVTYGGMAGRDMEALARGLYEGADEDYLHYRISQVQYLGERLREGGIPIQYPT
HCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECC
GGHAVFVDAAKILPHIPPEQFPAQVLCNALYLEAGIRAVEIGSLLLGRDPETGEQKASEL
CCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHCCCCCCCHHHHHHHH
ELMRLTIPRRVYTNDHMDYIADAIIELKNRAHELKGLTFEYEPPVLRHFTARFRELD
HHHEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]