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The map label for this gene is eno2 [H]

Identifier: 156977842

GI number: 156977842

Start: 1771800

End: 1773083

Strand: Direct

Name: eno2 [H]

Synonym: VIBHAR_06630

Alternate gene names: 156977842

Gene position: 1771800-1773083 (Clockwise)

Preceding gene: 156977840

Following gene: 156977844

Centisome position: 80.39

GC content: 49.77

Gene sequence:

>1284_bases
ATGGATTTCTTGATTGAGGATATTCTGGCGCGCGAAATTTTAGATTCACGCGGAAATCCGACTGTGGAAGTCGAAGTCAC
ACTCGGTTGCGGCATTGCAGCAAGAGCATCGGTGCCCTCGGGTGCCAGTACTGGTTCACGAGAAGCGGTTGAACTGCGAG
ATGGTGACGACACCCGCTTCGGTGGCAAAGGTGTACTCAATGCCGTTCAACTGATCAACACCGAAGTGCTTGAGGCTATT
GTTGGTTTAGACTCTCGAGATCAAGCACTGATTGATCGCATTATGATCGAGTTAGACGGCACCGAAAATAAGTCTCGTCT
GGGCGCAAACGGCATTCTTGGGGTTTCTTTGGCCGTAGCTCGCGCATCCGCGATGACAGCAGACATCCCACTCTATCAGT
ATCTCGGTGGTGTAGGCGCTAACCTTCTACCCGTGCCTTGCATGAACATCCTCAACGGTGGTGTACATGCTCGCTGGCAA
GGCCCGGATTTTCAGGAATACATGATCGCGCCATATGGAGCTAAAAACTTCAGACAAGCGGTGCAATGGGGCAGCGAAGT
CTATCATGCCCTACGTCAGGTTCTGCTTGAGTCAGATTACTCTGTTGGTGTCGGTGATGAAGGTGGCTTTGCTCCTGCAG
TGAAATCAAACCGCGAACCATTGGAGCTGATTGTCAAAGGGATTGAGAGAGCTGGACTGAAACCCGGTGTCGATGTAGGT
ATCTGTATGGACCCAGCCTCTAGTGAATTCTATGAGAATGGTAAATACAAGTTGCGCTCAGAGAACCGTGAACTGACCTC
CGCAGAGATGATTGAATACTACGAGAAGTTGGTTGAAGATTTTCCGGTCGTCCTTCTTGAAGATGGCTTGGCAGAAGACG
ATTGGGACAACTGGCCACTACTAAATGAGAAGCTTGGCGGTAAGATCGAAATCGTCGGTGACGATCTTCTCGTTACTAAT
ACCAAGTACATACAGAAAGCGATTGAGATGAATGCTGCCAACTCAGCATTGATCAAACTGAATCAGATTGGCTCTTTAAC
CGAAACCATTGAAGCGATTCAAATGTGTCATCACGCTGCATGGGGCGCGTTTGTTTCTCACCGCTCGGGGGAAACCACTG
ACAGCTTCATTGCCGATATGACAGTCGCACTTCGCACAGGTCACCTCAAAACAGGCGCACCATGCCGTGGTGAACGTGTC
GAAAAATACAACCAGCTCATGCGCATTGAAGACGAGTTAGGCTCAATGGCGATGTTTGCTGGCCGCAAAGCCTTCATTCG
CTAA

Upstream 100 bases:

>100_bases
CATCCTTGAACATCAAAGCTATCAAAAGCCGCCCACATTATAAATCGGAGTCTACACTCTAAAATAGTTTCTCCATCTAA
ATGATCATTAAGGACAAAAT

Downstream 100 bases:

>100_bases
TCCAACAATATGCCTAAAGCAAAGCCGCACAGTGTGGAACTGGGCGGCTTTTTAGTATCTGGAAAAATAATTACAAGGCT
TGTCGCATAATATGCTGAGG

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2 [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDFLIEDILAREILDSRGNPTVEVEVTLGCGIAARASVPSGASTGSREAVELRDGDDTRFGGKGVLNAVQLINTEVLEAI
VGLDSRDQALIDRIMIELDGTENKSRLGANGILGVSLAVARASAMTADIPLYQYLGGVGANLLPVPCMNILNGGVHARWQ
GPDFQEYMIAPYGAKNFRQAVQWGSEVYHALRQVLLESDYSVGVGDEGGFAPAVKSNREPLELIVKGIERAGLKPGVDVG
ICMDPASSEFYENGKYKLRSENRELTSAEMIEYYEKLVEDFPVVLLEDGLAEDDWDNWPLLNEKLGGKIEIVGDDLLVTN
TKYIQKAIEMNAANSALIKLNQIGSLTETIEAIQMCHHAAWGAFVSHRSGETTDSFIADMTVALRTGHLKTGAPCRGERV
EKYNQLMRIEDELGSMAMFAGRKAFIR

Sequences:

>Translated_427_residues
MDFLIEDILAREILDSRGNPTVEVEVTLGCGIAARASVPSGASTGSREAVELRDGDDTRFGGKGVLNAVQLINTEVLEAI
VGLDSRDQALIDRIMIELDGTENKSRLGANGILGVSLAVARASAMTADIPLYQYLGGVGANLLPVPCMNILNGGVHARWQ
GPDFQEYMIAPYGAKNFRQAVQWGSEVYHALRQVLLESDYSVGVGDEGGFAPAVKSNREPLELIVKGIERAGLKPGVDVG
ICMDPASSEFYENGKYKLRSENRELTSAEMIEYYEKLVEDFPVVLLEDGLAEDDWDNWPLLNEKLGGKIEIVGDDLLVTN
TKYIQKAIEMNAANSALIKLNQIGSLTETIEAIQMCHHAAWGAFVSHRSGETTDSFIADMTVALRTGHLKTGAPCRGERV
EKYNQLMRIEDELGSMAMFAGRKAFIR
>Mature_427_residues
MDFLIEDILAREILDSRGNPTVEVEVTLGCGIAARASVPSGASTGSREAVELRDGDDTRFGGKGVLNAVQLINTEVLEAI
VGLDSRDQALIDRIMIELDGTENKSRLGANGILGVSLAVARASAMTADIPLYQYLGGVGANLLPVPCMNILNGGVHARWQ
GPDFQEYMIAPYGAKNFRQAVQWGSEVYHALRQVLLESDYSVGVGDEGGFAPAVKSNREPLELIVKGIERAGLKPGVDVG
ICMDPASSEFYENGKYKLRSENRELTSAEMIEYYEKLVEDFPVVLLEDGLAEDDWDNWPLLNEKLGGKIEIVGDDLLVTN
TKYIQKAIEMNAANSALIKLNQIGSLTETIEAIQMCHHAAWGAFVSHRSGETTDSFIADMTVALRTGHLKTGAPCRGERV
EKYNQLMRIEDELGSMAMFAGRKAFIR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=51.3953488372093, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=51.3888888888889, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=51.3888888888889, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.4651162790698, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=46.2790697674419, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=22.9166666666667, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=22.9166666666667, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=22.9166666666667, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=52.8301886792453, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=50, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=50, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=50, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.6535796766744, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=48.5057471264368, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.8845265588915, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=48.2758620689655, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=48.2758620689655, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=48.5057471264368, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=48.5057471264368, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=48.5057471264368, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=48.5057471264368, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=48.8425925925926, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=48.8425925925926, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46529; Mature: 46529

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLIEDILAREILDSRGNPTVEVEVTLGCGIAARASVPSGASTGSREAVELRDGDDTRF
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCEEEEECCCCCCC
GGKGVLNAVQLINTEVLEAIVGLDSRDQALIDRIMIELDGTENKSRLGANGILGVSLAVA
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCHHCCCCCCHHHHHHHH
RASAMTADIPLYQYLGGVGANLLPVPCMNILNGGVHARWQGPDFQEYMIAPYGAKNFRQA
HHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHEECCCCHHHHHHH
VQWGSEVYHALRQVLLESDYSVGVGDEGGFAPAVKSNREPLELIVKGIERAGLKPGVDVG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
ICMDPASSEFYENGKYKLRSENRELTSAEMIEYYEKLVEDFPVVLLEDGLAEDDWDNWPL
EEECCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCC
LNEKLGGKIEIVGDDLLVTNTKYIQKAIEMNAANSALIKLNQIGSLTETIEAIQMCHHAA
CHHCCCCEEEEEECCEEECCHHHHHHHHHHCCCCHHEEEEHHHCCHHHHHHHHHHHHHHH
WGAFVSHRSGETTDSFIADMTVALRTGHLKTGAPCRGERVEKYNQLMRIEDELGSMAMFA
HHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GRKAFIR
CCHHHCC
>Mature Secondary Structure
MDFLIEDILAREILDSRGNPTVEVEVTLGCGIAARASVPSGASTGSREAVELRDGDDTRF
CCHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCEEEEECCCCCCC
GGKGVLNAVQLINTEVLEAIVGLDSRDQALIDRIMIELDGTENKSRLGANGILGVSLAVA
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCHHCCCCCCHHHHHHHH
RASAMTADIPLYQYLGGVGANLLPVPCMNILNGGVHARWQGPDFQEYMIAPYGAKNFRQA
HHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHEECCCCHHHHHHH
VQWGSEVYHALRQVLLESDYSVGVGDEGGFAPAVKSNREPLELIVKGIERAGLKPGVDVG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
ICMDPASSEFYENGKYKLRSENRELTSAEMIEYYEKLVEDFPVVLLEDGLAEDDWDNWPL
EEECCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCC
LNEKLGGKIEIVGDDLLVTNTKYIQKAIEMNAANSALIKLNQIGSLTETIEAIQMCHHAA
CHHCCCCEEEEEECCEEECCHHHHHHHHHHCCCCHHEEEEHHHCCHHHHHHHHHHHHHHH
WGAFVSHRSGETTDSFIADMTVALRTGHLKTGAPCRGERVEKYNQLMRIEDELGSMAMFA
HHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GRKAFIR
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]