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The map label for this gene is opuD [H]

Identifier: 156977748

GI number: 156977748

Start: 1689403

End: 1691022

Strand: Direct

Name: opuD [H]

Synonym: VIBHAR_06536

Alternate gene names: 156977748

Gene position: 1689403-1691022 (Clockwise)

Preceding gene: 156977746

Following gene: 156977750

Centisome position: 76.65

GC content: 48.77

Gene sequence:

>1620_bases
ATGCACGGCTTCCGAGCCCTAAAAAGCGAAGTAATTATGTCAAATATGACGAACGCCGCCTCTCATACTCCTATTCAGGA
GGCAGACTATTCTGCGATCCATCCTCCTTCCATTCTAAAGCGCCTTGAACTCACTAACCCAGTATTCTGGCTAAGTGGTA
GCTTCCTCACCGTGTTTGTATTGCTTGCTCTGACGAATACAGACTCTCTGACCGCAATGGTTAACGCAGGCTTCGGGTTT
GCGACTCAATACTTCGGTGCCTTCTGGCAAGTATTACTCCTTCTGAATTTCCTTATTGGTTTGGCTATTGCGCTTGGTCG
TACGGGTTACGTGCGCTTGGGTGGTTTAGCGAAACCGGATATCGATACCTTTAAATGGTTGTCGATCGTACTTTGTACCC
TACTCGCGGGTGGCGGTGTTTTCTGGGCTGCAGCTGAGCCTATCGCTCACTTTGTTTCTGCGCCACCGCTATATGGTGAA
GCTTCACCAAAAGCTTCTGCAATCAATGCACTTTCTCAGTCTTTCATGCACTGGGGCTTCCTTGCTTGGGCAATCTTAGG
TTGTCTTTCTTCTGTGGTTTTGATGCACCTTCACTACGATAAGGGCTTACCACTTAAACCACGTACTCTGCTCTATCCTG
TGTTTGGCAACAAGGCTATTAACGGTTGGATTGGTGATGTTACAGACGCCTGCAGCATCGTTGCCGTTGCGGCAGGTACC
ATTGGCCCTATCGGCTTCTTAGGTCTACAGATCAGTTACGCGTTGAACTCGCTATTTGGCTTGCCAGATACCTTCATCAC
CCAAAGCATAGTGATTGTAGCTGCAATTCTAATGTACACATTGTCAGCACTAAGCGGCGTGAGCAAAGGCATTCAGCTTG
TTAGCCGTTACAACATCGTGTTGTCTGTGGCTTTGATCGGTTACATCCTCGTTTTCGGGCCAACCAGCTTTATTGTTGAT
GGTTATGTACAGGGTATTGGCCGCATGGTCGATAACTTCTTCCCAATGGCACTGTATCGTGGCGACACGGAGTGGCTAAG
TTGGTGGACCGTCTTCTTCTGGGGCTGGTTCATTGGTTATGGCCCGATGATGGCAATCTTCATCGCGCGTATCTCACGTG
GCCGCACTATCCGTCAGTTGATCCTGTCTATCAGCATCGCTGCGCCTTTGATCACCTGCTTCTGGTTCAGCATTGTTGGC
GGCAGTGGTTTGGCGTTCGATCTAGAAAACCCAGGTTTGATTTCATCGGCATTTGAGGGCTTTAACTTGCCTGCCGTTCT
GCTTGCGATTACTTCTGAGCTGCCTTTCCCAATGGTAATTTCGGTATTGTTCTTGATCCTGACTACGACGTTTATCGTGA
CAACCGGTGACTCGATGACGTACACCATCAGTGTGGTAATGACAGGCTCTACTGAACCCAATGCCGTGATTCGCTCATTC
TGGGGCTTAATGATGGGTATCGTGGCTATTGCGCTTATCTCGATGGGCTCAGGTGGTATCTCTGCATTGCAGTCTTTCAT
CGTAATTACTGCCGTGCCTGTGTCTTTCATATTGCTACCAAGCATTTGGAAAGCGCCGAGCATCGCGAACCACATGGCGA
AAGAGCAAGGTTTGGTTTAA

Upstream 100 bases:

>100_bases
CAATTAATCGCGATCTGCGGTTATATTGAGCTTCTCAACGTGCTAGTATCAGCGGCTTTTTGATCATCCTCACACTTTTC
GAGCTATTGGTGTGAGGGAA

Downstream 100 bases:

>100_bases
CAACATAAACCAAGTCCTTCTCTCCAATTAGATGAGAAAACGGCCCCGACACATAAACTGTGTCGGGGCCGAATATTATG
GATTAGATTCGAAAAGCTTT

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MHGFRALKSEVIMSNMTNAASHTPIQEADYSAIHPPSILKRLELTNPVFWLSGSFLTVFVLLALTNTDSLTAMVNAGFGF
ATQYFGAFWQVLLLLNFLIGLAIALGRTGYVRLGGLAKPDIDTFKWLSIVLCTLLAGGGVFWAAAEPIAHFVSAPPLYGE
ASPKASAINALSQSFMHWGFLAWAILGCLSSVVLMHLHYDKGLPLKPRTLLYPVFGNKAINGWIGDVTDACSIVAVAAGT
IGPIGFLGLQISYALNSLFGLPDTFITQSIVIVAAILMYTLSALSGVSKGIQLVSRYNIVLSVALIGYILVFGPTSFIVD
GYVQGIGRMVDNFFPMALYRGDTEWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIAAPLITCFWFSIVG
GSGLAFDLENPGLISSAFEGFNLPAVLLAITSELPFPMVISVLFLILTTTFIVTTGDSMTYTISVVMTGSTEPNAVIRSF
WGLMMGIVAIALISMGSGGISALQSFIVITAVPVSFILLPSIWKAPSIANHMAKEQGLV

Sequences:

>Translated_539_residues
MHGFRALKSEVIMSNMTNAASHTPIQEADYSAIHPPSILKRLELTNPVFWLSGSFLTVFVLLALTNTDSLTAMVNAGFGF
ATQYFGAFWQVLLLLNFLIGLAIALGRTGYVRLGGLAKPDIDTFKWLSIVLCTLLAGGGVFWAAAEPIAHFVSAPPLYGE
ASPKASAINALSQSFMHWGFLAWAILGCLSSVVLMHLHYDKGLPLKPRTLLYPVFGNKAINGWIGDVTDACSIVAVAAGT
IGPIGFLGLQISYALNSLFGLPDTFITQSIVIVAAILMYTLSALSGVSKGIQLVSRYNIVLSVALIGYILVFGPTSFIVD
GYVQGIGRMVDNFFPMALYRGDTEWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIAAPLITCFWFSIVG
GSGLAFDLENPGLISSAFEGFNLPAVLLAITSELPFPMVISVLFLILTTTFIVTTGDSMTYTISVVMTGSTEPNAVIRSF
WGLMMGIVAIALISMGSGGISALQSFIVITAVPVSFILLPSIWKAPSIANHMAKEQGLV
>Mature_539_residues
MHGFRALKSEVIMSNMTNAASHTPIQEADYSAIHPPSILKRLELTNPVFWLSGSFLTVFVLLALTNTDSLTAMVNAGFGF
ATQYFGAFWQVLLLLNFLIGLAIALGRTGYVRLGGLAKPDIDTFKWLSIVLCTLLAGGGVFWAAAEPIAHFVSAPPLYGE
ASPKASAINALSQSFMHWGFLAWAILGCLSSVVLMHLHYDKGLPLKPRTLLYPVFGNKAINGWIGDVTDACSIVAVAAGT
IGPIGFLGLQISYALNSLFGLPDTFITQSIVIVAAILMYTLSALSGVSKGIQLVSRYNIVLSVALIGYILVFGPTSFIVD
GYVQGIGRMVDNFFPMALYRGDTEWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIAAPLITCFWFSIVG
GSGLAFDLENPGLISSAFEGFNLPAVLLAITSELPFPMVISVLFLILTTTFIVTTGDSMTYTISVVMTGSTEPNAVIRSF
WGLMMGIVAIALISMGSGGISALQSFIVITAVPVSFILLPSIWKAPSIANHMAKEQGLV

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=512, Percent_Identity=28.3203125, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786224, Length=468, Percent_Identity=24.7863247863248, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788102, Length=439, Percent_Identity=25.7403189066059, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 58052; Mature: 58052

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGFRALKSEVIMSNMTNAASHTPIQEADYSAIHPPSILKRLELTNPVFWLSGSFLTVFV
CCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHH
LLALTNTDSLTAMVNAGFGFATQYFGAFWQVLLLLNFLIGLAIALGRTGYVRLGGLAKPD
HHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
IDTFKWLSIVLCTLLAGGGVFWAAAEPIAHFVSAPPLYGEASPKASAINALSQSFMHWGF
CHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAWAILGCLSSVVLMHLHYDKGLPLKPRTLLYPVFGNKAINGWIGDVTDACSIVAVAAGT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
IGPIGFLGLQISYALNSLFGLPDTFITQSIVIVAAILMYTLSALSGVSKGIQLVSRYNIV
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVALIGYILVFGPTSFIVDGYVQGIGRMVDNFFPMALYRGDTEWLSWWTVFFWGWFIGY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GPMMAIFIARISRGRTIRQLILSISIAAPLITCFWFSIVGGSGLAFDLENPGLISSAFEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCC
FNLPAVLLAITSELPFPMVISVLFLILTTTFIVTTGDSMTYTISVVMTGSTEPNAVIRSF
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCHHHHHHH
WGLMMGIVAIALISMGSGGISALQSFIVITAVPVSFILLPSIWKAPSIANHMAKEQGLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MHGFRALKSEVIMSNMTNAASHTPIQEADYSAIHPPSILKRLELTNPVFWLSGSFLTVFV
CCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHH
LLALTNTDSLTAMVNAGFGFATQYFGAFWQVLLLLNFLIGLAIALGRTGYVRLGGLAKPD
HHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
IDTFKWLSIVLCTLLAGGGVFWAAAEPIAHFVSAPPLYGEASPKASAINALSQSFMHWGF
CHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAWAILGCLSSVVLMHLHYDKGLPLKPRTLLYPVFGNKAINGWIGDVTDACSIVAVAAGT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
IGPIGFLGLQISYALNSLFGLPDTFITQSIVIVAAILMYTLSALSGVSKGIQLVSRYNIV
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVALIGYILVFGPTSFIVDGYVQGIGRMVDNFFPMALYRGDTEWLSWWTVFFWGWFIGY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GPMMAIFIARISRGRTIRQLILSISIAAPLITCFWFSIVGGSGLAFDLENPGLISSAFEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCC
FNLPAVLLAITSELPFPMVISVLFLILTTTFIVTTGDSMTYTISVVMTGSTEPNAVIRSF
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCHHHHHHH
WGLMMGIVAIALISMGSGGISALQSFIVITAVPVSFILLPSIWKAPSIANHMAKEQGLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]