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The map label for this gene is ppsA [H]

Identifier: 156977729

GI number: 156977729

Start: 1672296

End: 1674683

Strand: Direct

Name: ppsA [H]

Synonym: VIBHAR_06517

Alternate gene names: 156977729

Gene position: 1672296-1674683 (Clockwise)

Preceding gene: 156977727

Following gene: 156977730

Centisome position: 75.87

GC content: 48.2

Gene sequence:

>2388_bases
ATGTTTCTGGAGAAAGACATGCAAAAGAACACCCTCTGGTTCAATGGACTATCCATGGATGATGTCGACAAGGTCGGCGG
TAAAAATGCTTCACTTGGCGAAATGGTTTCTAACCTAGCCAATGTTGGTGTATCAGTACCAAACGGTTTTGCGACAACCT
CGTATGCGTTTAACCAATTCCTTGACCACGAAGGTTTGGATGAGCGTATTCACCAACTACTTGATGAACTAGACGTTGAG
GATGTAGAAGCCCTGCGTAAGACAGGTGCCACCATTCGTCAATGGGTATTAGAAGCCCCGTTCCCTGCGGATCTTGAACA
AGACATCCGTGATAACTACAAAGAACTGACAGACAATCATCCTGAGATTTCTGTTGCCGTTCGCTCTTCTGCAACTGCAG
AGGATCTACCGGATGCGTCATTTGCTGGTCAGCAAGAAACGTTCCTAAACGTAAAAGGCATTGATGCAGTACTAGAAGCG
ACCAAGCACGTTTACGCTTCTCTGTTCAATGACCGCGCAATTTCATACCGTGTACACCAAGGCTTTAACCACCGCGGCAT
TTCGCTGTCGGCAGGTATCCAACGCATGGTGCGCTCGGACAAAGCCTCTTCTGGCGTTATGTTCACCCTAGATACCGAAT
CTGGCTTCGACCAAGTCGTGTTCATCACCTCTTCTTGGGGCTTGGGTGAAATGGTGGTTCAGGGTGCGGTTAACCCTGAT
GAATTCTACGTTCACAAGCCAATGCTAGAAGCAGGTCAACACCCTATCGTTAAGAAGACATTTGGCTCTAAGCAGATCAA
AATGATCTACTCAGACCGTCAAGAGATCGGTAAGCAAGTTGAGATCATCGACACCTCAGTAGAGGAGCGCAATGCCTTCT
CTCTGAATGATGAAGAGATCAAAGAGCTCGCAAAACAGGCGATGATCATCGAAAAGCACTACAAACGTCCTATGGACATT
GAGTGGGCAAAAGATGGCATCGACGGCAAGCTTTACATCGTACAGGCACGCCCAGAGACCGTATGTTCTCAAAGCGAGCA
AAACGTGATTGAACGCTACGAGCTAAGCAACAAAGCTGATGTTTTGGTTGAAGGTCGTGCAATTGGTCAGCGTATCGGTA
AAGGCCCAGTGCGCTTAGTCGATTCTCTAGACCAAATGTCATTGGTACAAGAAGGCGATGTACTGGTTACTGATATGACT
GACCCAGACTGGGAACCAGTAATGAAGAAGGCGTCTGCGATTGTCACGAACCGTGGCGGCCGTACTTGTCACGCAGCGAT
CATCGCACGTGAGCTTGGTATTCCGGCTATCGTTGGTTGTGGTGACGCCACCAGCAAACTGACTGACGGTGAGACCGTAA
CAGTATCTTGTGCTGAAGGCGAAACGGGCTACGTTTACCAAGGCGAGCTGGAGTTTGAAATCAAGCGCTCAGCGGTCGAT
GAGCTGCCACTGCTACCAACCAAAGTGATGATGAACGTTGGTAACCCAGATCGTGCATTCGACTTTGCTCAGATTCCAAA
CGAAGGTGTGGGCCTTGCGCGTCTTGAGTTCATCATCAACAAAATGATTGGTATTCACCCGAAAGCCTTGCTGAACTTCG
ATGCACAAAGCGATGAGCTAAAAGCTGAAATCACTGAGCGTATCCGTGGTTACAAAGATCCAATTGATTTCTACGTTAGC
AAGCTAACCGAAGGCATCGCGACTATCGCGTCGGCATTCTGGCCAAAACGCGTGATTGTTCGTATGTCTGATTTCAAATC
GAACGAGTACAGCAACTTGGTGGGCGGTAAAGGTTACGAACCGCATGAAGAGAACCCAATGCTGGGCTTCCGTGGTGCGT
CTCGCTACATTTCTCCAGTGTTTGAAGACTGTTTCGAGCTAGAAACTCAAGCAATCAAACGCGTTCGTAACGAGATGGGT
CTTAAGAACGTTGAAATCATGATCCCATTCGTACGTACACCAAGCGAAGCAGCTTCGGTTATCGACCTACTGGCTAAGTT
CGACCTACGTCGTGGCGACCAAGGTTTGAAAGTCATCATGATGTGTGAACTACCGTCAAACGCAGTACTTGCTGAAGAGT
TCTTGAAGTACTTCGATGGCTTCTCTATCGGTTCAAACGACATGACTCAGCTTACACTTGGTCTTGACCGTGACTCTGGC
GATGTTGCTCACCTATTCGATGAGCGTAACCCAGCAGTGAAAGCCATGCTGCAAATGGCCATAGATGCGGCAACCAAAGC
AGGCAAATATGTCGGTATCTGTGGCCAAGGTCCATCAGACCACGATGATCTTGCGGAATGGTTGATGGAACAAGGCATCA
GCTCTGTTTCACTCAACCCAGATACGGTTATCGATACTTGGTTGAGACTAGGTAAAGTGAGCAAGTAA

Upstream 100 bases:

>100_bases
AATGGCTGTTGTCAGCCCGAGACTGTGTCTTCGATGACATTGACCTGCAAGGATGAACCACCGCTGTTCAAGGTTGGTAT
TAATCCCCTACAAAGCTGCA

Downstream 100 bases:

>100_bases
AACGAGCCTTCCTAACTTGTAATACTAAGCCCTCAAGCTCCTGAGTTTGAGGGCTTTTTCTTTACCCAGCATGTCAAAGC
TCTGTGAGTTCGTGACGACA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MFLEKDMQKNTLWFNGLSMDDVDKVGGKNASLGEMVSNLANVGVSVPNGFATTSYAFNQFLDHEGLDERIHQLLDELDVE
DVEALRKTGATIRQWVLEAPFPADLEQDIRDNYKELTDNHPEISVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEA
TKHVYASLFNDRAISYRVHQGFNHRGISLSAGIQRMVRSDKASSGVMFTLDTESGFDQVVFITSSWGLGEMVVQGAVNPD
EFYVHKPMLEAGQHPIVKKTFGSKQIKMIYSDRQEIGKQVEIIDTSVEERNAFSLNDEEIKELAKQAMIIEKHYKRPMDI
EWAKDGIDGKLYIVQARPETVCSQSEQNVIERYELSNKADVLVEGRAIGQRIGKGPVRLVDSLDQMSLVQEGDVLVTDMT
DPDWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAIVGCGDATSKLTDGETVTVSCAEGETGYVYQGELEFEIKRSAVD
ELPLLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNFDAQSDELKAEITERIRGYKDPIDFYVS
KLTEGIATIASAFWPKRVIVRMSDFKSNEYSNLVGGKGYEPHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRNEMG
LKNVEIMIPFVRTPSEAASVIDLLAKFDLRRGDQGLKVIMMCELPSNAVLAEEFLKYFDGFSIGSNDMTQLTLGLDRDSG
DVAHLFDERNPAVKAMLQMAIDAATKAGKYVGICGQGPSDHDDLAEWLMEQGISSVSLNPDTVIDTWLRLGKVSK

Sequences:

>Translated_795_residues
MFLEKDMQKNTLWFNGLSMDDVDKVGGKNASLGEMVSNLANVGVSVPNGFATTSYAFNQFLDHEGLDERIHQLLDELDVE
DVEALRKTGATIRQWVLEAPFPADLEQDIRDNYKELTDNHPEISVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEA
TKHVYASLFNDRAISYRVHQGFNHRGISLSAGIQRMVRSDKASSGVMFTLDTESGFDQVVFITSSWGLGEMVVQGAVNPD
EFYVHKPMLEAGQHPIVKKTFGSKQIKMIYSDRQEIGKQVEIIDTSVEERNAFSLNDEEIKELAKQAMIIEKHYKRPMDI
EWAKDGIDGKLYIVQARPETVCSQSEQNVIERYELSNKADVLVEGRAIGQRIGKGPVRLVDSLDQMSLVQEGDVLVTDMT
DPDWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAIVGCGDATSKLTDGETVTVSCAEGETGYVYQGELEFEIKRSAVD
ELPLLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNFDAQSDELKAEITERIRGYKDPIDFYVS
KLTEGIATIASAFWPKRVIVRMSDFKSNEYSNLVGGKGYEPHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRNEMG
LKNVEIMIPFVRTPSEAASVIDLLAKFDLRRGDQGLKVIMMCELPSNAVLAEEFLKYFDGFSIGSNDMTQLTLGLDRDSG
DVAHLFDERNPAVKAMLQMAIDAATKAGKYVGICGQGPSDHDDLAEWLMEQGISSVSLNPDTVIDTWLRLGKVSK
>Mature_795_residues
MFLEKDMQKNTLWFNGLSMDDVDKVGGKNASLGEMVSNLANVGVSVPNGFATTSYAFNQFLDHEGLDERIHQLLDELDVE
DVEALRKTGATIRQWVLEAPFPADLEQDIRDNYKELTDNHPEISVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEA
TKHVYASLFNDRAISYRVHQGFNHRGISLSAGIQRMVRSDKASSGVMFTLDTESGFDQVVFITSSWGLGEMVVQGAVNPD
EFYVHKPMLEAGQHPIVKKTFGSKQIKMIYSDRQEIGKQVEIIDTSVEERNAFSLNDEEIKELAKQAMIIEKHYKRPMDI
EWAKDGIDGKLYIVQARPETVCSQSEQNVIERYELSNKADVLVEGRAIGQRIGKGPVRLVDSLDQMSLVQEGDVLVTDMT
DPDWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAIVGCGDATSKLTDGETVTVSCAEGETGYVYQGELEFEIKRSAVD
ELPLLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNFDAQSDELKAEITERIRGYKDPIDFYVS
KLTEGIATIASAFWPKRVIVRMSDFKSNEYSNLVGGKGYEPHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRNEMG
LKNVEIMIPFVRTPSEAASVIDLLAKFDLRRGDQGLKVIMMCELPSNAVLAEEFLKYFDGFSIGSNDMTQLTLGLDRDSG
DVAHLFDERNPAVKAMLQMAIDAATKAGKYVGICGQGPSDHDDLAEWLMEQGISSVSLNPDTVIDTWLRLGKVSK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=785, Percent_Identity=70.0636942675159, Blast_Score=1176, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=424, Percent_Identity=25.9433962264151, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788726, Length=378, Percent_Identity=26.4550264550265, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=31.0526315789474, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789193, Length=388, Percent_Identity=25.5154639175258, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=369, Percent_Identity=26.5582655826558, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 88191; Mature: 88191

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEKDMQKNTLWFNGLSMDDVDKVGGKNASLGEMVSNLANVGVSVPNGFATTSYAFNQF
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LDHEGLDERIHQLLDELDVEDVEALRKTGATIRQWVLEAPFPADLEQDIRDNYKELTDNH
HCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
PEISVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEATKHVYASLFNDRAISYRVHQ
CCEEEEEECCCCHHCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECC
GFNHRGISLSAGIQRMVRSDKASSGVMFTLDTESGFDQVVFITSSWGLGEMVVQGAVNPD
CCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCC
EFYVHKPMLEAGQHPIVKKTFGSKQIKMIYSDRQEIGKQVEIIDTSVEERNAFSLNDEEI
CEEEECCHHHCCCCCCCHHCCCCCCEEHHHHHHHHHCCEEEEEECCHHHCCCCCCCHHHH
KELAKQAMIIEKHYKRPMDIEWAKDGIDGKLYIVQARPETVCSQSEQNVIERYELSNKAD
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCCC
VLVEGRAIGQRIGKGPVRLVDSLDQMSLVQEGDVLVTDMTDPDWEPVMKKASAIVTNRGG
EEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGIPAIVGCGDATSKLTDGETVTVSCAEGETGYVYQGELEFEIKRSAVD
CCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEECCEEEEEHHHHHH
ELPLLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNFDAQSDEL
CCCCCCHHHHEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCHHH
KAEITERIRGYKDPIDFYVSKLTEGIATIASAFWPKRVIVRMSDFKSNEYSNLVGGKGYE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCHHHCCCCCCCC
PHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRNEMGLKNVEIMIPFVRTPSEAASV
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHH
IDLLAKFDLRRGDQGLKVIMMCELPSNAVLAEEFLKYFDGFSIGSNDMTQLTLGLDRDSG
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
DVAHLFDERNPAVKAMLQMAIDAATKAGKYVGICGQGPSDHDDLAEWLMEQGISSVSLNP
CHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEECCCC
DTVIDTWLRLGKVSK
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLEKDMQKNTLWFNGLSMDDVDKVGGKNASLGEMVSNLANVGVSVPNGFATTSYAFNQF
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LDHEGLDERIHQLLDELDVEDVEALRKTGATIRQWVLEAPFPADLEQDIRDNYKELTDNH
HCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
PEISVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEATKHVYASLFNDRAISYRVHQ
CCEEEEEECCCCHHCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECC
GFNHRGISLSAGIQRMVRSDKASSGVMFTLDTESGFDQVVFITSSWGLGEMVVQGAVNPD
CCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCC
EFYVHKPMLEAGQHPIVKKTFGSKQIKMIYSDRQEIGKQVEIIDTSVEERNAFSLNDEEI
CEEEECCHHHCCCCCCCHHCCCCCCEEHHHHHHHHHCCEEEEEECCHHHCCCCCCCHHHH
KELAKQAMIIEKHYKRPMDIEWAKDGIDGKLYIVQARPETVCSQSEQNVIERYELSNKAD
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCCCC
VLVEGRAIGQRIGKGPVRLVDSLDQMSLVQEGDVLVTDMTDPDWEPVMKKASAIVTNRGG
EEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGIPAIVGCGDATSKLTDGETVTVSCAEGETGYVYQGELEFEIKRSAVD
CCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEECCEEEEEHHHHHH
ELPLLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNFDAQSDEL
CCCCCCHHHHEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCHHH
KAEITERIRGYKDPIDFYVSKLTEGIATIASAFWPKRVIVRMSDFKSNEYSNLVGGKGYE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCHHHCCCCCCCC
PHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRNEMGLKNVEIMIPFVRTPSEAASV
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHH
IDLLAKFDLRRGDQGLKVIMMCELPSNAVLAEEFLKYFDGFSIGSNDMTQLTLGLDRDSG
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
DVAHLFDERNPAVKAMLQMAIDAATKAGKYVGICGQGPSDHDDLAEWLMEQGISSVSLNP
CHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEECCCC
DTVIDTWLRLGKVSK
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]