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The map label for this gene is dmlR [H]

Identifier: 156977706

GI number: 156977706

Start: 1646909

End: 1647814

Strand: Reverse

Name: dmlR [H]

Synonym: VIBHAR_06494

Alternate gene names: 156977706

Gene position: 1647814-1646909 (Counterclockwise)

Preceding gene: 156977712

Following gene: 156977702

Centisome position: 74.76

GC content: 47.57

Gene sequence:

>906_bases
ATGACGCTGTTTGTCAGCATAGTGAAGAACCAAGGGCTGGCGGCGGCTGGTAGAGAGCTCGGCTTATCGCCAGCAACAGT
AACGGCAAGATTGCAGTCGCTGGAAGACCGTTATGGGGTGAAATTACTTAATCGCAGTACAAGGCATATCTCACTGACGG
ACTCTGGCGCTATGTACCATCAGGCGTGTCTGGAAATCATCGACAGCGTTAAAGAGACGGAGAACTTACTCCAAACTGGC
ACCAAAGAAGTGCGCGGCACACTTAAAATCTCGGCTCCTCGTGATATTGGTAAGCAATATATTTCGCCAATACTGTCGGA
ATTTAGTCAGCTCTATCCCGATGTGATCCCTTATCTGTATTTGAACGATAATCTATCCAACTTGGCGGAGTCCGGTTTGG
ATATTGTTATCCGATATGGGGAGCTAGCCGACAGTAACCTTGTCTCTCGCAAATTGGCGTCCAGTCGCCGAGTGCTTTGC
GCTTCTCCTCAGTATTTGGCAAAGAAAGGCACGCCGATCACGCCACAAGATTTAGCACAGCACGATTGTTTAGCCATGGT
CCGCAGCAATGAAGAGTTGAAAACATGGCACTTTCAAGATGAAGAGCAGCACAATGTCATTACTGTCGTACCGAAGCGGT
TTTCTGATGATGGTGAAGTGATTCGTCAATGGGCGTTAGATGGCGCAGGTATTGCGTTGAAATCGATTCTCGATGTGCAA
GAGGACATCAAACAGCAGCGTTTAGTGACGGTGTTAAATGGCTATATGAAGAACTTCAACGCATCGACTTCTTCTGCAGG
CGCAGATTTGAACGTGATTTATCTGAGCCGCCAGTATCAACCCAAGCGACTTCGTTTGTTTTTGGACTTCTTGATTGAGC
GTTTTCATAGCCGGTTTCCGGAGTAA

Upstream 100 bases:

>100_bases
TTGACTGAAAGAAATTTGATAATTGGGTAATATTTGAATTGATTATTCAGTTCAGTCGAATAATGAGGTTTTAGGAGAGA
ATGTGGACAAGTTTTCGGAT

Downstream 100 bases:

>100_bases
GCAAGGCTTAGACGGCAACCTGCAATTGAGGCCATGTGATTAGCCAGTTTTTGGATTCTACCCGAGATTGAAACAGGGTT
AATATTCGCTTCGGATTGTA

Product: transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTLFVSIVKNQGLAAAGRELGLSPATVTARLQSLEDRYGVKLLNRSTRHISLTDSGAMYHQACLEIIDSVKETENLLQTG
TKEVRGTLKISAPRDIGKQYISPILSEFSQLYPDVIPYLYLNDNLSNLAESGLDIVIRYGELADSNLVSRKLASSRRVLC
ASPQYLAKKGTPITPQDLAQHDCLAMVRSNEELKTWHFQDEEQHNVITVVPKRFSDDGEVIRQWALDGAGIALKSILDVQ
EDIKQQRLVTVLNGYMKNFNASTSSAGADLNVIYLSRQYQPKRLRLFLDFLIERFHSRFPE

Sequences:

>Translated_301_residues
MTLFVSIVKNQGLAAAGRELGLSPATVTARLQSLEDRYGVKLLNRSTRHISLTDSGAMYHQACLEIIDSVKETENLLQTG
TKEVRGTLKISAPRDIGKQYISPILSEFSQLYPDVIPYLYLNDNLSNLAESGLDIVIRYGELADSNLVSRKLASSRRVLC
ASPQYLAKKGTPITPQDLAQHDCLAMVRSNEELKTWHFQDEEQHNVITVVPKRFSDDGEVIRQWALDGAGIALKSILDVQ
EDIKQQRLVTVLNGYMKNFNASTSSAGADLNVIYLSRQYQPKRLRLFLDFLIERFHSRFPE
>Mature_300_residues
TLFVSIVKNQGLAAAGRELGLSPATVTARLQSLEDRYGVKLLNRSTRHISLTDSGAMYHQACLEIIDSVKETENLLQTGT
KEVRGTLKISAPRDIGKQYISPILSEFSQLYPDVIPYLYLNDNLSNLAESGLDIVIRYGELADSNLVSRKLASSRRVLCA
SPQYLAKKGTPITPQDLAQHDCLAMVRSNEELKTWHFQDEEQHNVITVVPKRFSDDGEVIRQWALDGAGIALKSILDVQE
DIKQQRLVTVLNGYMKNFNASTSSAGADLNVIYLSRQYQPKRLRLFLDFLIERFHSRFPE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=32, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=29.6666666666667, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=31.5254237288136, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.719723183391, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=30.2013422818792, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789639, Length=259, Percent_Identity=32.046332046332, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789173, Length=282, Percent_Identity=27.6595744680851, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788706, Length=166, Percent_Identity=29.5180722891566, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=19.7952218430034, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786448, Length=256, Percent_Identity=26.171875, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33843; Mature: 33712

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFVSIVKNQGLAAAGRELGLSPATVTARLQSLEDRYGVKLLNRSTRHISLTDSGAMYH
CEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
QACLEIIDSVKETENLLQTGTKEVRGTLKISAPRDIGKQYISPILSEFSQLYPDVIPYLY
HHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
LNDNLSNLAESGLDIVIRYGELADSNLVSRKLASSRRVLCASPQYLAKKGTPITPQDLAQ
ECCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHH
HDCLAMVRSNEELKTWHFQDEEQHNVITVVPKRFSDDGEVIRQWALDGAGIALKSILDVQ
HHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
EDIKQQRLVTVLNGYMKNFNASTSSAGADLNVIYLSRQYQPKRLRLFLDFLIERFHSRFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
TLFVSIVKNQGLAAAGRELGLSPATVTARLQSLEDRYGVKLLNRSTRHISLTDSGAMYH
EEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHH
QACLEIIDSVKETENLLQTGTKEVRGTLKISAPRDIGKQYISPILSEFSQLYPDVIPYLY
HHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
LNDNLSNLAESGLDIVIRYGELADSNLVSRKLASSRRVLCASPQYLAKKGTPITPQDLAQ
ECCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHH
HDCLAMVRSNEELKTWHFQDEEQHNVITVVPKRFSDDGEVIRQWALDGAGIALKSILDVQ
HHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
EDIKQQRLVTVLNGYMKNFNASTSSAGADLNVIYLSRQYQPKRLRLFLDFLIERFHSRFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]