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The map label for this gene is opuD [H]

Identifier: 156977641

GI number: 156977641

Start: 1587745

End: 1589352

Strand: Direct

Name: opuD [H]

Synonym: VIBHAR_06429

Alternate gene names: 156977641

Gene position: 1587745-1589352 (Clockwise)

Preceding gene: 156977639

Following gene: 156977642

Centisome position: 72.04

GC content: 46.58

Gene sequence:

>1608_bases
ATGGATAATGCACTAAAAAAATATAGTATCGACACTACCGACTATCAGGTTGGTCAAGATAACGTTCAAAAATGGGGATT
TGATATCCACAATCCAGTATTCGGAATTAGTGCTGGTCTTGTTCTTCTCTGTCTTGCTTCTCTTCTCATTGTTGACCCTG
CTACTGCTCGTGACGCCTTAAATGGTTTAAAGAACAGCATCATTTCAGACTTCGACTCGTTCTTTATGTGGTCAGCAAAC
TTCTTTATCTTATTCGCTATCGCGATTCTTTTATCTCCTCTGGGTAAGATCAGAATCGGCGGTAAAGACGCCGTTGTGGA
ACATTCAACCGTCTCTTGGTTATCGATGCTTTTCGCTGCGGGTATGGGTATCGGCTTGTTGTTCTGGAGTGTTTCAGAAC
CAACGGCATACTTTACCAATTGGTTTGGTACCCCTCTCAATGTAGAACCTTACACGGAAGAAGCGAAATCACTCGCGATG
GCCGCCACCATGTTCCACTGGGGCATCCACGGTTGGGCGATTTACGCCATTGTTGCACTCTCGCTGGCCTTCTTCGCATT
CAATAAAGGTTTACCGCTTTCTCTGCGTTCTGCGTTTTATCCTATCTTTGGCGATCGCGCATGGGGTTGGTTAGGCCACG
TTATCGATATTCTGGCGGTACTCGCAACGCTATTTGGTCTCGCGACTTCTCTGGGCTTAGGTGCGCAACAAGCCACCAGC
GGTATTAACCATGTATTTGGTTTAGAAGGTGGTATTGGTACGCAAATGATTGTTATCGCCTTCGTTACTTTTATTGCAGT
ACTGTCTGTGATTCGAGGTATCGATGGAGGTGTCAAACTACTAAGTAACATCAATATGGCGGTTGCGTTTGCGCTGCTGG
CTTTTGTGATGTTTATTAGCTTCGATATTGCGACAACATCCATTTGGGATACCGCTGTCGCGTACGCACAGAATATTTTA
CCGCTAAGTAACCCTGCAGGTCGTGAAGACACAGAATGGATGCATGGCTGGACAGTATTCTACTGGGCATGGTGGGTATC
TTGGTCACCATTTGTGGGAATGTTCATCGCGCGCGTATCGAAAGGTCGTACTGTTCGCGAATTTATCTTCGCGGTTATTG
TCATTCCAACTACCGTTACACTTGTTTGGATGGCAGTCTTCGGTGGTATCGCCCTTGATCAAGTGGTGAACAAAGTCGGC
GAACTTGGTGCCAATGGATTAACGGATATCTCGCTTACTCTATTCCACGTTTATGACGCGATGCCGTTTAGCTCGATCAT
CTCTGTGCTCTCTATCGTCCTGATTTTGGTGTTCTTCATCACCTCATCAGATTCTGGCTCATTAGTTATCGATAGCATCA
CTGCCGGCGGTAAGATTGATGCACCAGTGCCACAACGTATTTTCTGGGCATGTATTGAAGGCTCTATCGCTGCCGTTATG
CTCTGGATTGGTGGTAAAGAAGCACTGCAAGCTCTGCAATCTGGTGTTGTCGCGACAGGTTTGCCATTCACATTGGTGCT
GTTAGTGATGTGTGTGAGCCTGATTAAAGGGTTAAGGTCTGAGTTACCTGCCTATCAAAACGCGGCGCAGAACGCGTCTG
TCGCCTAA

Upstream 100 bases:

>100_bases
CGTCGCATTTTTTAATTTGAACTCAAAATAATTGGAGGGCGGTTTTAGTGTTTTTCTTGTTCAAGAAGGACACATTTAAA
AAAGCGTATATGTTGTATTT

Downstream 100 bases:

>100_bases
TTCATCCTGAGCACAAGCGTATAGCAATACGGCTCATCGAAGGTTGAGATAACAAACGAGCGTCTATTTGGCGCTCGTTT
TTTGTTTCTGGCTAACCACG

Product: hypothetical protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MDNALKKYSIDTTDYQVGQDNVQKWGFDIHNPVFGISAGLVLLCLASLLIVDPATARDALNGLKNSIISDFDSFFMWSAN
FFILFAIAILLSPLGKIRIGGKDAVVEHSTVSWLSMLFAAGMGIGLLFWSVSEPTAYFTNWFGTPLNVEPYTEEAKSLAM
AATMFHWGIHGWAIYAIVALSLAFFAFNKGLPLSLRSAFYPIFGDRAWGWLGHVIDILAVLATLFGLATSLGLGAQQATS
GINHVFGLEGGIGTQMIVIAFVTFIAVLSVIRGIDGGVKLLSNINMAVAFALLAFVMFISFDIATTSIWDTAVAYAQNIL
PLSNPAGREDTEWMHGWTVFYWAWWVSWSPFVGMFIARVSKGRTVREFIFAVIVIPTTVTLVWMAVFGGIALDQVVNKVG
ELGANGLTDISLTLFHVYDAMPFSSIISVLSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVM
LWIGGKEALQALQSGVVATGLPFTLVLLVMCVSLIKGLRSELPAYQNAAQNASVA

Sequences:

>Translated_535_residues
MDNALKKYSIDTTDYQVGQDNVQKWGFDIHNPVFGISAGLVLLCLASLLIVDPATARDALNGLKNSIISDFDSFFMWSAN
FFILFAIAILLSPLGKIRIGGKDAVVEHSTVSWLSMLFAAGMGIGLLFWSVSEPTAYFTNWFGTPLNVEPYTEEAKSLAM
AATMFHWGIHGWAIYAIVALSLAFFAFNKGLPLSLRSAFYPIFGDRAWGWLGHVIDILAVLATLFGLATSLGLGAQQATS
GINHVFGLEGGIGTQMIVIAFVTFIAVLSVIRGIDGGVKLLSNINMAVAFALLAFVMFISFDIATTSIWDTAVAYAQNIL
PLSNPAGREDTEWMHGWTVFYWAWWVSWSPFVGMFIARVSKGRTVREFIFAVIVIPTTVTLVWMAVFGGIALDQVVNKVG
ELGANGLTDISLTLFHVYDAMPFSSIISVLSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVM
LWIGGKEALQALQSGVVATGLPFTLVLLVMCVSLIKGLRSELPAYQNAAQNASVA
>Mature_535_residues
MDNALKKYSIDTTDYQVGQDNVQKWGFDIHNPVFGISAGLVLLCLASLLIVDPATARDALNGLKNSIISDFDSFFMWSAN
FFILFAIAILLSPLGKIRIGGKDAVVEHSTVSWLSMLFAAGMGIGLLFWSVSEPTAYFTNWFGTPLNVEPYTEEAKSLAM
AATMFHWGIHGWAIYAIVALSLAFFAFNKGLPLSLRSAFYPIFGDRAWGWLGHVIDILAVLATLFGLATSLGLGAQQATS
GINHVFGLEGGIGTQMIVIAFVTFIAVLSVIRGIDGGVKLLSNINMAVAFALLAFVMFISFDIATTSIWDTAVAYAQNIL
PLSNPAGREDTEWMHGWTVFYWAWWVSWSPFVGMFIARVSKGRTVREFIFAVIVIPTTVTLVWMAVFGGIALDQVVNKVG
ELGANGLTDISLTLFHVYDAMPFSSIISVLSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVM
LWIGGKEALQALQSGVVATGLPFTLVLLVMCVSLIKGLRSELPAYQNAAQNASVA

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=520, Percent_Identity=35.5769230769231, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1788102, Length=452, Percent_Identity=30.3097345132743, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1786224, Length=482, Percent_Identity=26.7634854771784, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57764; Mature: 57764

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNALKKYSIDTTDYQVGQDNVQKWGFDIHNPVFGISAGLVLLCLASLLIVDPATARDAL
CCCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NGLKNSIISDFDSFFMWSANFFILFAIAILLSPLGKIRIGGKDAVVEHSTVSWLSMLFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHH
GMGIGLLFWSVSEPTAYFTNWFGTPLNVEPYTEEAKSLAMAATMFHWGIHGWAIYAIVAL
CCCHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAFFAFNKGLPLSLRSAFYPIFGDRAWGWLGHVIDILAVLATLFGLATSLGLGAQQATS
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GINHVFGLEGGIGTQMIVIAFVTFIAVLSVIRGIDGGVKLLSNINMAVAFALLAFVMFIS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHH
FDIATTSIWDTAVAYAQNILPLSNPAGREDTEWMHGWTVFYWAWWVSWSPFVGMFIARVS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGRTVREFIFAVIVIPTTVTLVWMAVFGGIALDQVVNKVGELGANGLTDISLTLFHVYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
MPFSSIISVLSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVM
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHH
LWIGGKEALQALQSGVVATGLPFTLVLLVMCVSLIKGLRSELPAYQNAAQNASVA
EECCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MDNALKKYSIDTTDYQVGQDNVQKWGFDIHNPVFGISAGLVLLCLASLLIVDPATARDAL
CCCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NGLKNSIISDFDSFFMWSANFFILFAIAILLSPLGKIRIGGKDAVVEHSTVSWLSMLFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHH
GMGIGLLFWSVSEPTAYFTNWFGTPLNVEPYTEEAKSLAMAATMFHWGIHGWAIYAIVAL
CCCHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAFFAFNKGLPLSLRSAFYPIFGDRAWGWLGHVIDILAVLATLFGLATSLGLGAQQATS
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GINHVFGLEGGIGTQMIVIAFVTFIAVLSVIRGIDGGVKLLSNINMAVAFALLAFVMFIS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHH
FDIATTSIWDTAVAYAQNILPLSNPAGREDTEWMHGWTVFYWAWWVSWSPFVGMFIARVS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGRTVREFIFAVIVIPTTVTLVWMAVFGGIALDQVVNKVGELGANGLTDISLTLFHVYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
MPFSSIISVLSIVLILVFFITSSDSGSLVIDSITAGGKIDAPVPQRIFWACIEGSIAAVM
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHH
LWIGGKEALQALQSGVVATGLPFTLVLLVMCVSLIKGLRSELPAYQNAAQNASVA
EECCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]