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The map label for this gene is sseA [H]

Identifier: 156977465

GI number: 156977465

Start: 1430108

End: 1430938

Strand: Direct

Name: sseA [H]

Synonym: VIBHAR_06252

Alternate gene names: 156977465

Gene position: 1430108-1430938 (Clockwise)

Preceding gene: 156977463

Following gene: 156977466

Centisome position: 64.89

GC content: 45.25

Gene sequence:

>831_bases
ATGCAAGCACTGATCAGTACTGAAGAACTTAATAGCCTACTCGGCCAACCTAACGTCAAACTCCTCGACGCGAGTATTGC
GTTTCAAATCCCTTCAGAAGGCGAAAAAGTTAAAGATAAGTGGCTTCCTAATTCCCTACGCTTTGATTACGACAACGATT
TTTGCTTACCTGATTCATCTCTGCCACATATGATGCCAACAGAAGAAGGCTTTAACCAAAGCGCGCAACAGCTCGGTTTG
AACAACGAAGATTTGATCGTGGTATACGACAACTCAGGGACACTTGCCGCACCAAGAGCATGGTGGATGTTCAGAGCAAT
GAGTCATGAGAATGTTCGAGTTCTGAACGGCGGTCTTCCCACTTGGATTAAAACTGGGCTTCCTGTTACGAGTGCTTTAT
CGCAACCAAGCGGAAAAGGTAACTTTTCAGGCAAGCTCAACCAAGAAGCGTTTTTGAGCGCCCAAGCTGTACTTGCTCAT
TCGAATAACCAAAGTGCAAACATCATCGATGCAAGAGCCAAAGCTCGTTTCCTTGGTGAAGTGCCAGAACCACGAGAAGG
GCTACGCAGTGGGCATATTCCAAATTCGGTTTGCTTACCGTTTCAAGAATTCTTGACCAACGGTCACATTAAACCGAATT
CAGAGTTGCAACAGATATTTAGCTCACTAACAATAGATAGTGATAATTCAATCATTTTTAGTTGTGGCTCCGGTGTAACC
GCATGCATTTTGTTACTTGCGGCGTATCAAATTGGTCTGGACAACCTGTCGGTGTACGACGGTTCTTGGACCGAATGGGG
TGCAGACCATTCTCTACCTATCGAGCGTTAA

Upstream 100 bases:

>100_bases
GATTCACTCTTCGAGCAACCGCTCACGTTTTTAAGCTGTGAATAAGCTTACTCTTCACAGTTATTTAAGGCGCCATACTA
CTTTGAGGAAACAAAAGACG

Downstream 100 bases:

>100_bases
GATCAGGAGCAGTAAAACGATATGATCGGCATAAAGAAGAACATTTGGACGCTCTACATGACGCTGTTTGCGATAAGTGT
GGGTTTGTTCTCTCTGTTTG

Product: thiosulfate sulfurtransferase SseA

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQALISTEELNSLLGQPNVKLLDASIAFQIPSEGEKVKDKWLPNSLRFDYDNDFCLPDSSLPHMMPTEEGFNQSAQQLGL
NNEDLIVVYDNSGTLAAPRAWWMFRAMSHENVRVLNGGLPTWIKTGLPVTSALSQPSGKGNFSGKLNQEAFLSAQAVLAH
SNNQSANIIDARAKARFLGEVPEPREGLRSGHIPNSVCLPFQEFLTNGHIKPNSELQQIFSSLTIDSDNSIIFSCGSGVT
ACILLLAAYQIGLDNLSVYDGSWTEWGADHSLPIER

Sequences:

>Translated_276_residues
MQALISTEELNSLLGQPNVKLLDASIAFQIPSEGEKVKDKWLPNSLRFDYDNDFCLPDSSLPHMMPTEEGFNQSAQQLGL
NNEDLIVVYDNSGTLAAPRAWWMFRAMSHENVRVLNGGLPTWIKTGLPVTSALSQPSGKGNFSGKLNQEAFLSAQAVLAH
SNNQSANIIDARAKARFLGEVPEPREGLRSGHIPNSVCLPFQEFLTNGHIKPNSELQQIFSSLTIDSDNSIIFSCGSGVT
ACILLLAAYQIGLDNLSVYDGSWTEWGADHSLPIER
>Mature_276_residues
MQALISTEELNSLLGQPNVKLLDASIAFQIPSEGEKVKDKWLPNSLRFDYDNDFCLPDSSLPHMMPTEEGFNQSAQQLGL
NNEDLIVVYDNSGTLAAPRAWWMFRAMSHENVRVLNGGLPTWIKTGLPVTSALSQPSGKGNFSGKLNQEAFLSAQAVLAH
SNNQSANIIDARAKARFLGEVPEPREGLRSGHIPNSVCLPFQEFLTNGHIKPNSELQQIFSSLTIDSDNSIIFSCGSGVT
ACILLLAAYQIGLDNLSVYDGSWTEWGADHSLPIER

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=276, Percent_Identity=38.4057971014493, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI194473668, Length=279, Percent_Identity=36.2007168458781, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI61835204, Length=279, Percent_Identity=36.2007168458781, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI194473681, Length=279, Percent_Identity=36.2007168458781, Blast_Score=180, Evalue=1e-45,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=38.7096774193548, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI87081967, Length=209, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543836, Length=264, Percent_Identity=26.5151515151515, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17543838, Length=264, Percent_Identity=26.5151515151515, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=236, Percent_Identity=26.271186440678, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=233, Percent_Identity=23.6051502145923, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559150, Length=217, Percent_Identity=26.2672811059908, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115534702, Length=204, Percent_Identity=24.0196078431373, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=246, Percent_Identity=32.520325203252, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30216; Mature: 30216

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALISTEELNSLLGQPNVKLLDASIAFQIPSEGEKVKDKWLPNSLRFDYDNDFCLPDSS
CCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHHCCCCCEEECCCCCEECCCCC
LPHMMPTEEGFNQSAQQLGLNNEDLIVVYDNSGTLAAPRAWWMFRAMSHENVRVLNGGLP
CCCCCCCHHHCCHHHHHHCCCCCCEEEEECCCCCEECCHHHHHHHHHCCCCEEEEECCCC
TWIKTGLPVTSALSQPSGKGNFSGKLNQEAFLSAQAVLAHSNNQSANIIDARAKARFLGE
HHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHCC
VPEPREGLRSGHIPNSVCLPFQEFLTNGHIKPNSELQQIFSSLTIDSDNSIIFSCGSGVT
CCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
ACILLLAAYQIGLDNLSVYDGSWTEWGADHSLPIER
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQALISTEELNSLLGQPNVKLLDASIAFQIPSEGEKVKDKWLPNSLRFDYDNDFCLPDSS
CCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHHCCCCCEEECCCCCEECCCCC
LPHMMPTEEGFNQSAQQLGLNNEDLIVVYDNSGTLAAPRAWWMFRAMSHENVRVLNGGLP
CCCCCCCHHHCCHHHHHHCCCCCCEEEEECCCCCEECCHHHHHHHHHCCCCEEEEECCCC
TWIKTGLPVTSALSQPSGKGNFSGKLNQEAFLSAQAVLAHSNNQSANIIDARAKARFLGE
HHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHCC
VPEPREGLRSGHIPNSVCLPFQEFLTNGHIKPNSELQQIFSSLTIDSDNSIIFSCGSGVT
CCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
ACILLLAAYQIGLDNLSVYDGSWTEWGADHSLPIER
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]