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The map label for this gene is fre [H]

Identifier: 156977452

GI number: 156977452

Start: 1419813

End: 1420532

Strand: Reverse

Name: fre [H]

Synonym: VIBHAR_06239

Alternate gene names: 156977452

Gene position: 1420532-1419813 (Counterclockwise)

Preceding gene: 156977453

Following gene: 156977451

Centisome position: 64.45

GC content: 44.17

Gene sequence:

>720_bases
ATGAAGGAGGCAACTGACATGTTATGTTCGATAGAAAAAATTGAGCCGTTAACTAACCTCATATTCCGAGTATTACTCAA
GCCAGATCAGCCTTTTGAATTTAGGGCAGGGCAGTACATTAACGTCAGTTTAAGCTTTGGTAGTTTACCGTTTTCTATAG
CCTCATGTCCTTCTAATGGTGCGTTTTTAGAACTCCATATTGGTGGCTCAGATATCAGCAAGAAAAATACGCTTGTGATG
GAAGAACTCACCAATTCGTGGATCTGCGGCAACATGGTTGAAGTCAGTGAGGCGCGAGGCGACGCTTGGCTGCGTGATGA
GAGTGTCAAACCCTTGTTATTGGTCGCAGGCGGGACCGGAATGTCTTACACCCTAAGTATTTTGAAAAATAGCTTGGAGC
AAGGGTTTATCCAGCCGATTTACGTCTATTGGGGCGCTAAGGATATGGATAACCTGTATGTACATGACGAACTGGTGGAT
ATTGCGCTTGAAAACAAAAATGTCAGTTACGTGCCAGTCACTGAAATATCAACCTGTCCCCAATACGCTAAGCAAGGAAA
GGTGTTGGAGTGTGTGATGAGTGATTTCCGTAACTTATCTGAGTTCGATATCTACTTGTGTGGTCCTTGCAAAATGGTTG
AAGTGGCTCGTGATTGGTTCTGTGACAAAAGAGGGGCAGAACCAGAGCAAATTTACGCGGACGCGTTCGCTTATTTGTAA

Upstream 100 bases:

>100_bases
GGCGATCGCCCGGGAACCACCGTTGAGATCGTTAGAAGAGTTAAGACGCGGGGTATGAAGGGGTGTGCTCTCAGTATGTC
TCAAGCATTTACAGCTAAGA

Downstream 100 bases:

>100_bases
TCATTATCAAGGAGAAGAAACTATGAGCTCAACGTCACTACTAGATGAGTTTGGCACTCCAGTACAAAGGGTAGAAAGAG
CGATTGAGGCTCTGAAAAAT

Product: FMN reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKEATDMLCSIEKIEPLTNLIFRVLLKPDQPFEFRAGQYINVSLSFGSLPFSIASCPSNGAFLELHIGGSDISKKNTLVM
EELTNSWICGNMVEVSEARGDAWLRDESVKPLLLVAGGTGMSYTLSILKNSLEQGFIQPIYVYWGAKDMDNLYVHDELVD
IALENKNVSYVPVTEISTCPQYAKQGKVLECVMSDFRNLSEFDIYLCGPCKMVEVARDWFCDKRGAEPEQIYADAFAYL

Sequences:

>Translated_239_residues
MKEATDMLCSIEKIEPLTNLIFRVLLKPDQPFEFRAGQYINVSLSFGSLPFSIASCPSNGAFLELHIGGSDISKKNTLVM
EELTNSWICGNMVEVSEARGDAWLRDESVKPLLLVAGGTGMSYTLSILKNSLEQGFIQPIYVYWGAKDMDNLYVHDELVD
IALENKNVSYVPVTEISTCPQYAKQGKVLECVMSDFRNLSEFDIYLCGPCKMVEVARDWFCDKRGAEPEQIYADAFAYL
>Mature_239_residues
MKEATDMLCSIEKIEPLTNLIFRVLLKPDQPFEFRAGQYINVSLSFGSLPFSIASCPSNGAFLELHIGGSDISKKNTLVM
EELTNSWICGNMVEVSEARGDAWLRDESVKPLLLVAGGTGMSYTLSILKNSLEQGFIQPIYVYWGAKDMDNLYVHDELVD
IALENKNVSYVPVTEISTCPQYAKQGKVLECVMSDFRNLSEFDIYLCGPCKMVEVARDWFCDKRGAEPEQIYADAFAYL

Specific function: Probable flavin reductase in the luminescent systems of different marine bacteria [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=236, Percent_Identity=37.2881355932203, Blast_Score=177, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 26804; Mature: 26804

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEATDMLCSIEKIEPLTNLIFRVLLKPDQPFEFRAGQYINVSLSFGSLPFSIASCPSNG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCC
AFLELHIGGSDISKKNTLVMEELTNSWICGNMVEVSEARGDAWLRDESVKPLLLVAGGTG
CEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCEEEHHCCCCCEECCCCCCEEEEEECCCC
MSYTLSILKNSLEQGFIQPIYVYWGAKDMDNLYVHDELVDIALENKNVSYVPVTEISTCP
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHEEEEEECCCCCEEEECCCCCCCH
QYAKQGKVLECVMSDFRNLSEFDIYLCGPCKMVEVARDWFCDKRGAEPEQIYADAFAYL
HHHHCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKEATDMLCSIEKIEPLTNLIFRVLLKPDQPFEFRAGQYINVSLSFGSLPFSIASCPSNG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCC
AFLELHIGGSDISKKNTLVMEELTNSWICGNMVEVSEARGDAWLRDESVKPLLLVAGGTG
CEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCEEEHHCCCCCEECCCCCCEEEEEECCCC
MSYTLSILKNSLEQGFIQPIYVYWGAKDMDNLYVHDELVDIALENKNVSYVPVTEISTCP
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHEEEEEECCCCCEEEECCCCCCCH
QYAKQGKVLECVMSDFRNLSEFDIYLCGPCKMVEVARDWFCDKRGAEPEQIYADAFAYL
HHHHCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2303459; 2775296 [H]