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The map label for this gene is nfnB [C]

Identifier: 156977388

GI number: 156977388

Start: 1354063

End: 1354719

Strand: Direct

Name: nfnB [C]

Synonym: VIBHAR_06175

Alternate gene names: 156977388

Gene position: 1354063-1354719 (Clockwise)

Preceding gene: 156977387

Following gene: 156977395

Centisome position: 61.44

GC content: 45.36

Gene sequence:

>657_bases
GTGTCTCATCAAATCATTACAGACCTAAACACCCGTTACACAGCGAAAAAATACGACGAAGAGAAACGCATCTCTCAAGA
AGATATGGCGATCATCAAAGAATCCATTCGCCTATCTGCATCTTCTATCAACTCTCAGCCTTGGAAATTCATCGTAATTG
AGAGCGACGAAGCGAAACAGCGTTTTCACGACACATTTGCTAACATGCACCAGTTCAACCAACCGCATGCTAAAGCAGCA
TCTCACACTATTCTGCTAGCGCACGACCCAAAATTCACTAAAGAGAAATACGCGAAGCGTGTTGATGCTGAGGTAACTTC
CGGTCACCTACCCGCAGACAAGTACAATATGTTCATGGGCAATTACGCATTTGCTGAAGCAAACACAGATGAGAACGGCT
ACAACGACGGTTGGACGAAAGCGCAAGTTTACATCGCTCTTGGTAACCTAATGCACACACTAGCGCGTCTAGGTATCGAC
TCAACTCCAATGGAAGGTGTAGACCCAACACTTATCGGCGAAGAGTTCAAAGCAGAACTAGATGGTCACGTATGTGAAAT
TGCACTTGCAATCGGCTATCACAAAGACGGCGAAGATTACAACCACGGTCTACCTAAGGCGCGTCTACACATGGATGACG
TGATTACTACTCTTTAA

Upstream 100 bases:

>100_bases
TTCGAGAAAAGTCGATATGATGGACACACTTTCGAGGTACGCATGGTTACCGATGCTAAGCGCATCCTCATTTGTCTTAT
AATATTAAGAGGATATAAAC

Downstream 100 bases:

>100_bases
TAAGTACCGTCTCCTGGCGCCTTGGCTTCTAACCTAGCTATCTAAATCATTGTTCAAATTGAAAGAGCCGTACTTCTTAG
TCTAAGAGCACGGCTCTTTT

Product: NAD(P)H-dependent flavin reductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: FRASE I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSHQIITDLNTRYTAKKYDEEKRISQEDMAIIKESIRLSASSINSQPWKFIVIESDEAKQRFHDTFANMHQFNQPHAKAA
SHTILLAHDPKFTKEKYAKRVDAEVTSGHLPADKYNMFMGNYAFAEANTDENGYNDGWTKAQVYIALGNLMHTLARLGID
STPMEGVDPTLIGEEFKAELDGHVCEIALAIGYHKDGEDYNHGLPKARLHMDDVITTL

Sequences:

>Translated_218_residues
MSHQIITDLNTRYTAKKYDEEKRISQEDMAIIKESIRLSASSINSQPWKFIVIESDEAKQRFHDTFANMHQFNQPHAKAA
SHTILLAHDPKFTKEKYAKRVDAEVTSGHLPADKYNMFMGNYAFAEANTDENGYNDGWTKAQVYIALGNLMHTLARLGID
STPMEGVDPTLIGEEFKAELDGHVCEIALAIGYHKDGEDYNHGLPKARLHMDDVITTL
>Mature_217_residues
SHQIITDLNTRYTAKKYDEEKRISQEDMAIIKESIRLSASSINSQPWKFIVIESDEAKQRFHDTFANMHQFNQPHAKAAS
HTILLAHDPKFTKEKYAKRVDAEVTSGHLPADKYNMFMGNYAFAEANTDENGYNDGWTKAQVYIALGNLMHTLARLGIDS
TPMEGVDPTLIGEEFKAELDGHVCEIALAIGYHKDGEDYNHGLPKARLHMDDVITTL

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Major FMN reductase. It is capable of using both NADH and NADPH as electron donors. As electron acceptor, FMN is the most effectiv

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=214, Percent_Identity=30.8411214953271, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24657; Mature: 24526

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQIITDLNTRYTAKKYDEEKRISQEDMAIIKESIRLSASSINSQPWKFIVIESDEAKQ
CCCCEEECCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
RFHDTFANMHQFNQPHAKAASHTILLAHDPKFTKEKYAKRVDAEVTSGHLPADKYNMFMG
HHHHHHHHHHHCCCCCHHHHCCEEEEEECCCHHHHHHHHHHCCHHCCCCCCCHHHHHEEC
NYAFAEANTDENGYNDGWTKAQVYIALGNLMHTLARLGIDSTPMEGVDPTLIGEEFKAEL
CEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DGHVCEIALAIGYHKDGEDYNHGLPKARLHMDDVITTL
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SHQIITDLNTRYTAKKYDEEKRISQEDMAIIKESIRLSASSINSQPWKFIVIESDEAKQ
CCCEEECCCCCHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
RFHDTFANMHQFNQPHAKAASHTILLAHDPKFTKEKYAKRVDAEVTSGHLPADKYNMFMG
HHHHHHHHHHHCCCCCHHHHCCEEEEEECCCHHHHHHHHHHCCHHCCCCCCCHHHHHEEC
NYAFAEANTDENGYNDGWTKAQVYIALGNLMHTLARLGIDSTPMEGVDPTLIGEEFKAEL
CEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DGHVCEIALAIGYHKDGEDYNHGLPKARLHMDDVITTL
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206830; 8033996; 9654450 [H]