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The map label for this gene is cmlA [H]

Identifier: 156977248

GI number: 156977248

Start: 1214573

End: 1215655

Strand: Direct

Name: cmlA [H]

Synonym: VIBHAR_06034

Alternate gene names: 156977248

Gene position: 1214573-1215655 (Clockwise)

Preceding gene: 156977245

Following gene: 156977252

Centisome position: 55.11

GC content: 41.74

Gene sequence:

>1083_bases
ATGGTTCAGCGCATTTTGAGCTACAACTTTATTGGTATCTGTCTCGGCTCTTCATTATACGGTCCTCTATCCGATCATTT
TGGCCGCAGAAAGGTGTTAATATTTGGTTTTAGCATTTTTCTTACCGCAAGCATTGGCTGTATCTTTTCGACAAGTATTG
AACAGTTGCTGGCATTTCGACTATTGCAAGGCTTTGGAAGCTCAGCATGTGTGATAGTGGGTACGGCAATGATCTTCGAT
TTATTCGAGGAAGAATCAGCAGCAAAACTCATTTCAGACTTAAACACATTGGTGGTTAGTCTCATGGCTTTTGCTCCACT
CATTGGTGGATGGATCAGCTTACATATGGGCTATAAAGCAAACTTCGTTTTTATCGCAGTTCTCGTGCTTATTTCCGCAC
TCAAATGTTTCCTGTTCTTGCCAGAATCTCTCGCTGTGAACAAAAGAAAGAAACTAGCACCAAAGCGTATTCTCAGTGAT
TATAAGACGGTCTTATCTTCCACTGAGTTTTGGTACAACACATTAGTCACCAGTTTGATCCTTGGTGCCTACATGATTTA
TGTGTCAAATATGTCCTTGCTGTATATCGATCAGTTGGGGGTTAGCCCAAAGCAGTTCCCGTTTTACCAAATGGCGACAC
TCGGCACATTTGTCTTGGTAAGTTTGAACAACTCACGCTTGATAGATAAGTTCGGTGTTGAAAAGCTTAAAGCAACTGGC
CTTGGACTGATATTCGCTAATACCCTGATATTTTTCATATCACCGTCGAATTTACTGGATTCACCAGTATTCTTAACGGC
AACCATGTGTTTGTTTTCGATTGGTGCAGGCTTATCGATAGGTTTATTTTTTGCAAAATCAATGCAGGTATTTCCAGAAC
TCACGGGCATTGCGGCATCTCTAGTGACAGCGATAAGACTGTTTATAGTATCTGCCGCTATCGATATTGGCAGTAATGCT
TACAAGGGAACATCGCAGTCAGTCGTCCAAATCATGACGCTTGTCGTTCTCGTTTTGGCTATCCTTTGGCTGGCGAATTA
TGTGCACATGAAAAAAATGTCAGGTTTATCAGACGCCAGCTAA

Upstream 100 bases:

>100_bases
TTTTGTTCGAGGAAAATAGCTGAAAAACATTGAAGTCACGCTTGCAGCATTGAAATATTATGTTACATTCATACTCAGCG
ATTTCAACACCAATGCTGTT

Downstream 100 bases:

>100_bases
TCACTCGTAAAATAAGGAGAGCATGTCAACGCTCTCCTTTTCACTGCAGGGGCCTTATAGATTCTTAATGATCTACTTGG
AACGATGTTGGGAATAAACT

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MVQRILSYNFIGICLGSSLYGPLSDHFGRRKVLIFGFSIFLTASIGCIFSTSIEQLLAFRLLQGFGSSACVIVGTAMIFD
LFEEESAAKLISDLNTLVVSLMAFAPLIGGWISLHMGYKANFVFIAVLVLISALKCFLFLPESLAVNKRKKLAPKRILSD
YKTVLSSTEFWYNTLVTSLILGAYMIYVSNMSLLYIDQLGVSPKQFPFYQMATLGTFVLVSLNNSRLIDKFGVEKLKATG
LGLIFANTLIFFISPSNLLDSPVFLTATMCLFSIGAGLSIGLFFAKSMQVFPELTGIAASLVTAIRLFIVSAAIDIGSNA
YKGTSQSVVQIMTLVVLVLAILWLANYVHMKKMSGLSDAS

Sequences:

>Translated_360_residues
MVQRILSYNFIGICLGSSLYGPLSDHFGRRKVLIFGFSIFLTASIGCIFSTSIEQLLAFRLLQGFGSSACVIVGTAMIFD
LFEEESAAKLISDLNTLVVSLMAFAPLIGGWISLHMGYKANFVFIAVLVLISALKCFLFLPESLAVNKRKKLAPKRILSD
YKTVLSSTEFWYNTLVTSLILGAYMIYVSNMSLLYIDQLGVSPKQFPFYQMATLGTFVLVSLNNSRLIDKFGVEKLKATG
LGLIFANTLIFFISPSNLLDSPVFLTATMCLFSIGAGLSIGLFFAKSMQVFPELTGIAASLVTAIRLFIVSAAIDIGSNA
YKGTSQSVVQIMTLVVLVLAILWLANYVHMKKMSGLSDAS
>Mature_360_residues
MVQRILSYNFIGICLGSSLYGPLSDHFGRRKVLIFGFSIFLTASIGCIFSTSIEQLLAFRLLQGFGSSACVIVGTAMIFD
LFEEESAAKLISDLNTLVVSLMAFAPLIGGWISLHMGYKANFVFIAVLVLISALKCFLFLPESLAVNKRKKLAPKRILSD
YKTVLSSTEFWYNTLVTSLILGAYMIYVSNMSLLYIDQLGVSPKQFPFYQMATLGTFVLVSLNNSRLIDKFGVEKLKATG
LGLIFANTLIFFISPSNLLDSPVFLTATMCLFSIGAGLSIGLFFAKSMQVFPELTGIAASLVTAIRLFIVSAAIDIGSNA
YKGTSQSVVQIMTLVVLVLAILWLANYVHMKKMSGLSDAS

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=245, Percent_Identity=24.0816326530612, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1790794, Length=353, Percent_Identity=27.1954674220963, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994889, Length=307, Percent_Identity=23.7785016286645, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787065, Length=340, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790146, Length=277, Percent_Identity=24.5487364620939, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082312, Length=152, Percent_Identity=30.9210526315789, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 39260; Mature: 39260

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQRILSYNFIGICLGSSLYGPLSDHFGRRKVLIFGFSIFLTASIGCIFSTSIEQLLAFR
CCCHHHHCCHHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGFGSSACVIVGTAMIFDLFEEESAAKLISDLNTLVVSLMAFAPLIGGWISLHMGYKA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NFVFIAVLVLISALKCFLFLPESLAVNKRKKLAPKRILSDYKTVLSSTEFWYNTLVTSLI
HHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAYMIYVSNMSLLYIDQLGVSPKQFPFYQMATLGTFVLVSLNNSRLIDKFGVEKLKATG
HHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCC
LGLIFANTLIFFISPSNLLDSPVFLTATMCLFSIGAGLSIGLFFAKSMQVFPELTGIAAS
HHHHHHHHHHEEECCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVTAIRLFIVSAAIDIGSNAYKGTSQSVVQIMTLVVLVLAILWLANYVHMKKMSGLSDAS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVQRILSYNFIGICLGSSLYGPLSDHFGRRKVLIFGFSIFLTASIGCIFSTSIEQLLAFR
CCCHHHHCCHHHHHHCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGFGSSACVIVGTAMIFDLFEEESAAKLISDLNTLVVSLMAFAPLIGGWISLHMGYKA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NFVFIAVLVLISALKCFLFLPESLAVNKRKKLAPKRILSDYKTVLSSTEFWYNTLVTSLI
HHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAYMIYVSNMSLLYIDQLGVSPKQFPFYQMATLGTFVLVSLNNSRLIDKFGVEKLKATG
HHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCC
LGLIFANTLIFFISPSNLLDSPVFLTATMCLFSIGAGLSIGLFFAKSMQVFPELTGIAAS
HHHHHHHHHHEEECCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LVTAIRLFIVSAAIDIGSNAYKGTSQSVVQIMTLVVLVLAILWLANYVHMKKMSGLSDAS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]