Click here to switch to the map view.

The map label for this gene is yfmE [H]

Identifier: 156977223

GI number: 156977223

Start: 1195041

End: 1196012

Strand: Direct

Name: yfmE [H]

Synonym: VIBHAR_06009

Alternate gene names: 156977223

Gene position: 1195041-1196012 (Clockwise)

Preceding gene: 156977222

Following gene: 156977224

Centisome position: 54.22

GC content: 47.74

Gene sequence:

>972_bases
ATGAACAAAACAAAAGCAATTACGTTAACCATTTTATTAATTGCTTGTGCGATAGGCAGCTTATTCGTCGGTAGTGTCTC
CCTTTCCTTAAGTGACGTGGTAACTACATTACTTCACGGTGGTGACTTCGATTTCATTATTAATCAATACCGACTGCCAC
GTTTTATCTTAGCGATTGGCGTTGGTGCTGGACTCGGGCTCTCTGGAGCATTGATCCAAGGAGTCTTGAGAAACCCTCTC
GCCTCACCAGATTTAATGGGTATCAGCGCGGGTACTGGTTTTGCTGCAACCTTGCTGCTTGTTTATGTGCCAGACGCATC
ATTGCCGTTGCTTGCCTCTGTTGCCATTTCCGGAGGAGTTACCGCCGCGCTTGGTATTATTGGTATCAGCTATAAATACA
ACCCCACCCCAGCAAGGCTCGCATTGATTGGCATTGCAATCAGCACCTTCATTTCTAGCGGTATCGATTTTCTGCTGATT
GTTAACCCGGTAGAAATTAATACGGCAATGGTCTGGTTAACCGGAAGCTTATGGGGGCGTAACTGGTCACAAGTGCCATT
GATTTGGATATCACTGGCCATCTTACTGCCTCTTGCGTTGCGACTGGCTTGGAAACTCGACTTAATGGGGCTTGGGGAAG
AAGCCGCCACCACAATAGGTGCTCAACCAGAGCGCACACGCTTTATCGCATTGGTTCTTTCCGTTCTCCTTGCCAGTATT
AGCGTTGCAGTATGTGGGACGATCAGCTTCGTGGGCTTGCTATCTCCTCATTTAGCTCGCTTATTGTTTGGACACAATCA
CAAGTACTTGCTCCCTATGTCTGGTGTGATTGGGGCTTTACTTGTGGTTGTCGCAGACGCCGCCGCTCGTGGTATTGCTC
CCCCACTTGAACTTCCGGCTGGAGTACTGACTTCGCTCATTGGTGCACCTTACTTCATCTTCTTACTTCAACGCTATAAA
GGTTGGAAATAA

Upstream 100 bases:

>100_bases
CACGCATGGTCGCGTTCCCAGCAGAAACGCCAGTCGGGGTTATTACTGCATTGATTGGTTCGCCATTCTTCATTGCACTG
GCCGTTAGGAGCAAAGTGTC

Downstream 100 bases:

>100_bases
TGCTTAAAACACATCAGCTGTCTGTTTCTTATGGCAAAGAAAACATTATTCATAACCTCGATCTCGACATTCCAGAAGGA
AAGATCACAGCATTAATTGG

Product: ABC-type Fe3+-siderophore transporter permease

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate import system permease protein [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNKTKAITLTILLIACAIGSLFVGSVSLSLSDVVTTLLHGGDFDFIINQYRLPRFILAIGVGAGLGLSGALIQGVLRNPL
ASPDLMGISAGTGFAATLLLVYVPDASLPLLASVAISGGVTAALGIIGISYKYNPTPARLALIGIAISTFISSGIDFLLI
VNPVEINTAMVWLTGSLWGRNWSQVPLIWISLAILLPLALRLAWKLDLMGLGEEAATTIGAQPERTRFIALVLSVLLASI
SVAVCGTISFVGLLSPHLARLLFGHNHKYLLPMSGVIGALLVVVADAAARGIAPPLELPAGVLTSLIGAPYFIFLLQRYK
GWK

Sequences:

>Translated_323_residues
MNKTKAITLTILLIACAIGSLFVGSVSLSLSDVVTTLLHGGDFDFIINQYRLPRFILAIGVGAGLGLSGALIQGVLRNPL
ASPDLMGISAGTGFAATLLLVYVPDASLPLLASVAISGGVTAALGIIGISYKYNPTPARLALIGIAISTFISSGIDFLLI
VNPVEINTAMVWLTGSLWGRNWSQVPLIWISLAILLPLALRLAWKLDLMGLGEEAATTIGAQPERTRFIALVLSVLLASI
SVAVCGTISFVGLLSPHLARLLFGHNHKYLLPMSGVIGALLVVVADAAARGIAPPLELPAGVLTSLIGAPYFIFLLQRYK
GWK
>Mature_323_residues
MNKTKAITLTILLIACAIGSLFVGSVSLSLSDVVTTLLHGGDFDFIINQYRLPRFILAIGVGAGLGLSGALIQGVLRNPL
ASPDLMGISAGTGFAATLLLVYVPDASLPLLASVAISGGVTAALGIIGISYKYNPTPARLALIGIAISTFISSGIDFLLI
VNPVEINTAMVWLTGSLWGRNWSQVPLIWISLAILLPLALRLAWKLDLMGLGEEAATTIGAQPERTRFIALVLSVLLASI
SVAVCGTISFVGLLSPHLARLLFGHNHKYLLPMSGVIGALLVVVADAAARGIAPPLELPAGVLTSLIGAPYFIFLLQRYK
GWK

Specific function: Part of the ABC transporter complex yfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790740, Length=320, Percent_Identity=42.5, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1790741, Length=306, Percent_Identity=34.640522875817, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786347, Length=274, Percent_Identity=32.8467153284672, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786804, Length=319, Percent_Identity=32.6018808777429, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786805, Length=304, Percent_Identity=33.2236842105263, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788004, Length=281, Percent_Identity=33.8078291814947, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 33813; Mature: 33813

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTKAITLTILLIACAIGSLFVGSVSLSLSDVVTTLLHGGDFDFIINQYRLPRFILAIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
VGAGLGLSGALIQGVLRNPLASPDLMGISAGTGFAATLLLVYVPDASLPLLASVAISGGV
CCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHEEHEECCCCCCHHHHHHHHCCCH
TAALGIIGISYKYNPTPARLALIGIAISTFISSGIDFLLIVNPVEINTAMVWLTGSLWGR
HHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECEEECEEEEEEECHHCCC
NWSQVPLIWISLAILLPLALRLAWKLDLMGLGEEAATTIGAQPERTRFIALVLSVLLASI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SVAVCGTISFVGLLSPHLARLLFGHNHKYLLPMSGVIGALLVVVADAAARGIAPPLELPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCH
GVLTSLIGAPYFIFLLQRYKGWK
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKTKAITLTILLIACAIGSLFVGSVSLSLSDVVTTLLHGGDFDFIINQYRLPRFILAIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
VGAGLGLSGALIQGVLRNPLASPDLMGISAGTGFAATLLLVYVPDASLPLLASVAISGGV
CCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHEEHEECCCCCCHHHHHHHHCCCH
TAALGIIGISYKYNPTPARLALIGIAISTFISSGIDFLLIVNPVEINTAMVWLTGSLWGR
HHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECEEECEEEEEEECHHCCC
NWSQVPLIWISLAILLPLALRLAWKLDLMGLGEEAATTIGAQPERTRFIALVLSVLLASI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SVAVCGTISFVGLLSPHLARLLFGHNHKYLLPMSGVIGALLVVVADAAARGIAPPLELPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCH
GVLTSLIGAPYFIFLLQRYKGWK
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]