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The map label for this gene is insA [H]

Identifier: 156977202

GI number: 156977202

Start: 1175245

End: 1175520

Strand: Direct

Name: insA [H]

Synonym: VIBHAR_05988

Alternate gene names: 156977202

Gene position: 1175245-1175520 (Clockwise)

Preceding gene: 156977201

Following gene: 156977203

Centisome position: 53.32

GC content: 42.75

Gene sequence:

>276_bases
ATGGCTACTATCCAAGTCCAATGTCGTTTCTGTAATAAAACTGAGTCCGTCCGTAAACACGGAAAGGGGCATTCTGGCTT
TCCTCGTTTCAGATGTATCGAGTGTCGAAAGAGCTTTCAACTTGATTACGTTTACGAAGCTCGCAAGCCAAATGTAAAAG
AAAAAATTGTCGATATGGCAATGAATAGCTCTGGCGTTAGGGAAACAGCAGGAGTGTTGAATGTCGCATACAACACTGTG
TTGAGCACTTTAAAAAACTCTCGCCAAGGCAAGTGA

Upstream 100 bases:

>100_bases
AAATAAAACAAAACAAAGAGGAAATATATTTTTAGAGGTCGTGACTCAAATCGGTTGAAGCGTGTTAATGTAGCCTCCTG
ATTTTTGATGTTTTTCAATC

Downstream 100 bases:

>100_bases
CTTCGATCCCCTTCGGTCTTTGTTGCTTAAATCGTTGGAACTAAATCAAGAAGCTAGGATCTGATCGGCTACACCTTTCA
ATCGTCGTAGCCTTATGTCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MATIQVQCRFCNKTESVRKHGKGHSGFPRFRCIECRKSFQLDYVYEARKPNVKEKIVDMAMNSSGVRETAGVLNVAYNTV
LSTLKNSRQGK

Sequences:

>Translated_91_residues
MATIQVQCRFCNKTESVRKHGKGHSGFPRFRCIECRKSFQLDYVYEARKPNVKEKIVDMAMNSSGVRETAGVLNVAYNTV
LSTLKNSRQGK
>Mature_90_residues
ATIQVQCRFCNKTESVRKHGKGHSGFPRFRCIECRKSFQLDYVYEARKPNVKEKIVDMAMNSSGVRETAGVLNVAYNTVL
STLKNSRQGK

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=87, Percent_Identity=43.6781609195402, Blast_Score=89, Evalue=6e-20,
Organism=Escherichia coli, GI1788204, Length=87, Percent_Identity=43.6781609195402, Blast_Score=89, Evalue=6e-20,
Organism=Escherichia coli, GI1786204, Length=87, Percent_Identity=43.6781609195402, Blast_Score=89, Evalue=6e-20,
Organism=Escherichia coli, GI1790747, Length=87, Percent_Identity=45.9770114942529, Blast_Score=88, Evalue=8e-20,
Organism=Escherichia coli, GI87081814, Length=87, Percent_Identity=42.5287356321839, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786471, Length=87, Percent_Identity=42.5287356321839, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786460, Length=87, Percent_Identity=42.5287356321839, Blast_Score=87, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10344; Mature: 10213

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIQVQCRFCNKTESVRKHGKGHSGFPRFRCIECRKSFQLDYVYEARKPNVKEKIVDMA
CCEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
MNSSGVRETAGVLNVAYNTVLSTLKNSRQGK
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATIQVQCRFCNKTESVRKHGKGHSGFPRFRCIECRKSFQLDYVYEARKPNVKEKIVDMA
CEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
MNSSGVRETAGVLNVAYNTVLSTLKNSRQGK
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]