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The map label for this gene is focA [H]

Identifier: 156977137

GI number: 156977137

Start: 1111512

End: 1112963

Strand: Direct

Name: focA [H]

Synonym: VIBHAR_05923

Alternate gene names: 156977137

Gene position: 1111512-1112963 (Clockwise)

Preceding gene: 156977135

Following gene: 156977141

Centisome position: 50.43

GC content: 44.77

Gene sequence:

>1452_bases
ATGGCGGTGACCAGCTCTCAAAATAACCAGTATTTTTCTCCAAAAGAAATGATGGCTGAAGCTGAGAAGTTTGCACTTAG
TAAAGCAAACAAAACCAGCGGCATGACACTGGGTTTGGCAATCATGGCAGGCGCATTCATCGGTTTAGCATTCTTGTTTT
ACATCACAGTGACAACAGGTAGTGCGGATGCAGGTTGGGGGCTTAGCCGTCTTGCAGGTGGTTTAGCATTCAGTATGGGT
TTGATACTCATCGTCATTTGTGGTGGTGAGTTGTTTACCAGCTCAGTGCTTTCGAGCATCTCATGGGCAAACAAACAAAT
CTCATTCGGCAAAATGCTGTCGATCTGGGGCAAGGTGTATGTGGGTAACTTCATCGGTGCGATGTTCCTACTTGCATTGG
TAAGCGCTGCAGGCTTGTACCAAATGGATGCGGGTCAATGGGGCTTAAACGCACTAAACATTGCGCAGCACAAGCTACAC
CACACATTGGTACAAGCATTTGCACTGGGGATTCTATGTAACTTATTGGTTTGTCTTGCTATTTGGCTAACGTTCAGTTC
TGCAAATGCAATGACAAAAGCAGCGATGACCATCATGCCAGTGGCAATGTTCGTCAGCAGTGGCTTTGAGCACTGTGTAG
CAAACATGTTCATGGTACCGCTAGGCATCGTAATCGCGAATTTCGCACCAGAGACTTTCTGGACAGCAGTTGGCGTGCCG
GCTTCTCAATATGCAGATTTAAATGTCGGTCACTTTATTACTGCAAACCTAATTCCAGTAACCCTAGGCAACATCATTGG
TGGCGCTGTGTTAGTTGGCTTAGCTAACTGGTGTATTTACCGTCGACCAGAACTAAAGGCAGCAAAAATTTCATCCATTA
CAAACACTACAACACTAACGTCAGTTAAGGAAAGCACTATGAAAAACGCATCAATTGTAAAAGAAATCATGAACCCGAGC
CCAGTTACTCTGAGCGTAGAAATGCCAGTTTCAACTGCACTAGATACCCTATTGTACAACAATCTAATCAGTGCTCCTGT
TGTTGACATCGACAATCGTTTGGTTGGTTTCTTTTCAGCGCACGACGTAATGGTTGAATTGTGGTGCCAAGATTACATCC
CTGTTCAAGAACAAAAAGTGGTAGATTTGATGAGCCGTGACGTTGTAGCGATTGATGCTGGTGACCGTTTGGTAGATGTA
GTTGAGTTCCTATGTATCGATAAGGAACAACTTTACCCAACAACAAGTATGGGCATGGCGACACGCTTTACTTCTCTTTC
TCTTGAAGAGCGTGCTAAGAGCATCAAAGTGAGCAAACCACAAGTGCTTCCTGTATTAGAAAACGGTTTGATGGCTGGCG
TTGTGACACGTAGCGAAGTACTTAAAGCACTGCGCCCAGTGTATGGCGAGCGTCTAAACCTAATTGAAGATAACGTTCTA
GAAACGGCATAA

Upstream 100 bases:

>100_bases
CCGCAACAAAAAGCAATAACACCTTCACTTTCGAGTACAGACAACGTCTCGATTTCACTAACCAAGGAGTTCATATGGAA
CCACAGACTAAGGAGTCATT

Downstream 100 bases:

>100_bases
AACTATTACAACTAGCAACTAGCAAGACAAGTTGGCACCAATAAAAAGCGCTCTTATTTAAGAGCGCTTTCTGCATTTAT
GTCGTTAAGAACGAGGTATA

Product: methionine gamma-lyase

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAVTSSQNNQYFSPKEMMAEAEKFALSKANKTSGMTLGLAIMAGAFIGLAFLFYITVTTGSADAGWGLSRLAGGLAFSMG
LILIVICGGELFTSSVLSSISWANKQISFGKMLSIWGKVYVGNFIGAMFLLALVSAAGLYQMDAGQWGLNALNIAQHKLH
HTLVQAFALGILCNLLVCLAIWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIANFAPETFWTAVGVP
ASQYADLNVGHFITANLIPVTLGNIIGGAVLVGLANWCIYRRPELKAAKISSITNTTTLTSVKESTMKNASIVKEIMNPS
PVTLSVEMPVSTALDTLLYNNLISAPVVDIDNRLVGFFSAHDVMVELWCQDYIPVQEQKVVDLMSRDVVAIDAGDRLVDV
VEFLCIDKEQLYPTTSMGMATRFTSLSLEERAKSIKVSKPQVLPVLENGLMAGVVTRSEVLKALRPVYGERLNLIEDNVL
ETA

Sequences:

>Translated_483_residues
MAVTSSQNNQYFSPKEMMAEAEKFALSKANKTSGMTLGLAIMAGAFIGLAFLFYITVTTGSADAGWGLSRLAGGLAFSMG
LILIVICGGELFTSSVLSSISWANKQISFGKMLSIWGKVYVGNFIGAMFLLALVSAAGLYQMDAGQWGLNALNIAQHKLH
HTLVQAFALGILCNLLVCLAIWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIANFAPETFWTAVGVP
ASQYADLNVGHFITANLIPVTLGNIIGGAVLVGLANWCIYRRPELKAAKISSITNTTTLTSVKESTMKNASIVKEIMNPS
PVTLSVEMPVSTALDTLLYNNLISAPVVDIDNRLVGFFSAHDVMVELWCQDYIPVQEQKVVDLMSRDVVAIDAGDRLVDV
VEFLCIDKEQLYPTTSMGMATRFTSLSLEERAKSIKVSKPQVLPVLENGLMAGVVTRSEVLKALRPVYGERLNLIEDNVL
ETA
>Mature_482_residues
AVTSSQNNQYFSPKEMMAEAEKFALSKANKTSGMTLGLAIMAGAFIGLAFLFYITVTTGSADAGWGLSRLAGGLAFSMGL
ILIVICGGELFTSSVLSSISWANKQISFGKMLSIWGKVYVGNFIGAMFLLALVSAAGLYQMDAGQWGLNALNIAQHKLHH
TLVQAFALGILCNLLVCLAIWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIANFAPETFWTAVGVPA
SQYADLNVGHFITANLIPVTLGNIIGGAVLVGLANWCIYRRPELKAAKISSITNTTTLTSVKESTMKNASIVKEIMNPSP
VTLSVEMPVSTALDTLLYNNLISAPVVDIDNRLVGFFSAHDVMVELWCQDYIPVQEQKVVDLMSRDVVAIDAGDRLVDVV
EFLCIDKEQLYPTTSMGMATRFTSLSLEERAKSIKVSKPQVLPVLENGLMAGVVTRSEVLKALRPVYGERLNLIEDNVLE
TA

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=269, Percent_Identity=51.6728624535316, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1788837, Length=266, Percent_Identity=40.9774436090226, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082249, Length=276, Percent_Identity=31.1594202898551, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321779, Length=272, Percent_Identity=26.4705882352941, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 52043; Mature: 51911

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTSSQNNQYFSPKEMMAEAEKFALSKANKTSGMTLGLAIMAGAFIGLAFLFYITVTTG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC
SADAGWGLSRLAGGLAFSMGLILIVICGGELFTSSVLSSISWANKQISFGKMLSIWGKVY
CCCCCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VGNFIGAMFLLALVSAAGLYQMDAGQWGLNALNIAQHKLHHTLVQAFALGILCNLLVCLA
HHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIANFAPETFWTAVGVP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
ASQYADLNVGHFITANLIPVTLGNIIGGAVLVGLANWCIYRRPELKAAKISSITNTTTLT
HHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHH
SVKESTMKNASIVKEIMNPSPVTLSVEMPVSTALDTLLYNNLISAPVVDIDNRLVGFFSA
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
HDVMVELWCQDYIPVQEQKVVDLMSRDVVAIDAGDRLVDVVEFLCIDKEQLYPTTSMGMA
HHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHHCCCCCCCCHH
TRFTSLSLEERAKSIKVSKPQVLPVLENGLMAGVVTRSEVLKALRPVYGERLNLIEDNVL
HHHHHCCHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETA
CCC
>Mature Secondary Structure 
AVTSSQNNQYFSPKEMMAEAEKFALSKANKTSGMTLGLAIMAGAFIGLAFLFYITVTTG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC
SADAGWGLSRLAGGLAFSMGLILIVICGGELFTSSVLSSISWANKQISFGKMLSIWGKVY
CCCCCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VGNFIGAMFLLALVSAAGLYQMDAGQWGLNALNIAQHKLHHTLVQAFALGILCNLLVCLA
HHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWLTFSSANAMTKAAMTIMPVAMFVSSGFEHCVANMFMVPLGIVIANFAPETFWTAVGVP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
ASQYADLNVGHFITANLIPVTLGNIIGGAVLVGLANWCIYRRPELKAAKISSITNTTTLT
HHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHH
SVKESTMKNASIVKEIMNPSPVTLSVEMPVSTALDTLLYNNLISAPVVDIDNRLVGFFSA
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
HDVMVELWCQDYIPVQEQKVVDLMSRDVVAIDAGDRLVDVVEFLCIDKEQLYPTTSMGMA
HHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHHCCCCCCCCHH
TRFTSLSLEERAKSIKVSKPQVLPVLENGLMAGVVTRSEVLKALRPVYGERLNLIEDNVL
HHHHHCCHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]