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The map label for this gene is flbD [H]

Identifier: 156977088

GI number: 156977088

Start: 1065035

End: 1066663

Strand: Direct

Name: flbD [H]

Synonym: VIBHAR_05873

Alternate gene names: 156977088

Gene position: 1065035-1066663 (Clockwise)

Preceding gene: 156977087

Following gene: 156977096

Centisome position: 48.32

GC content: 47.58

Gene sequence:

>1629_bases
ATGCGTTCAGCTAATCATAGCGAACTTAGCAACGCCCTACTCGCGATCAGCCAATCTTTGGCTGATCGCAGCCAGCTGAC
CCAAACGTTAGATGCCGTGTTGACTGCGGCGAGACAAATGACACTCGCAAAGCATGGCATCATTTACGTTTTAGACCAGA
CTGGTCAAGCGTTGATCCCAAGTACCGCTCACCATAACGATAAAACGATTGTTTCCCACCCTTGGGAAGCATTGCAGATA
GATTCTGCCAGTGAGAACGACCCATTTAACTTTGCGATTCGCAATGGTGAAGTGGTGTTGATCAACGAGCTGTACAAGTA
CAACGGTTACGACTGTGAAAGCATTTATCAAAGTGAGCAAATACTTGGACTAAAGAGTGAAAACTTATTGGCTTGGCCAC
TTATCGACAGCGATAGTAAAACCATCGGCCTGTTAGTATTGCTCGATTTAAGCGTTATCGACAACGAAGCCGCACTCACT
GAGTTCTGTCGTATGGCTGCAAGCAATATTCGCCAAGCGGTGTGGTTAGAGCAATATGGGCAAGTGATCAAAAGCCTGAG
TGCCGACAATCAAGCTCTAGTGCGCGAGAACACTCAGCTTAAAAAACGAACCAAGAAAACCTACCAAGGTCCGATTGCAG
AGAGCGAAGAAATGGTCAATGTCTTGAATCGTCTTGATAAGGTGCTGTCTCTTCCTGTCGACGTTCTGTTGCGCGGTGAA
ACTGGTGCAGGTAAAGAAGTGATCGCGAAATACATTCACGAGAACTCTAACCGAGCAGACCAGCCTTTGATCGTTCAAAA
CTGTGCGGCTATTCCAGAGCAGTTATTGGAATCGGAACTTTTTGGGCATAAGAAAGGCTCATTCACGGGCGCAGACAAAG
ATAAAGTGGGCCTGTTTGAAGCAGCCAATGGTGGCACGCTATTCCTAGACGAGATTGGTGATATGCCAATGCTACTTCAA
GCTAAGCTACTTCGAGTACTGCAAGAGCGTAAGGTTCGCCCTATCGGTGCCAGTAAAGAGATTGAGGTAGACGTTCGCGT
TATCGCAGCCACCCACTGCAATCTTATGCAGCAGATCAAAGACGGCGGGTTCCGTGCTGACTTGTTCTATCGCTTGAATG
TATTCCCGATTACGCTGCCTCCATTGAGAGAACGTGAGTCAGACATTATTCCTCTCGCAGAACATTTCGTGCAGCACACG
ACCAAGACCCTTGGCTTACCGCAAGCGCCGGGGTTAAGTGCCAACGTGCGTAAACAGCTTCTTGCATACTCTTATCCGGG
TAACGTACGTGAGCTGAAAAACATCATCGAGCGCTCAGTACTACTGTCCGATTTTGAAACCATCACTCACATCGAGTTTG
GTGAACAAATCCCAGAAGACACGCCAAGTGTTGAATTAAAAGCGGCATCACCTACCCCAGAACAACCCAATTACGAGCCA
GTACAAGCGGATTTAGAAGACTCGGCAATCGGCTTAAAGGACGTTGTTAGCCAATACGAACGCACAGTGATCATCGACTG
CTTAAATGCCTGTAATTGGCACACCAAGAAAGCCGCCGAGCAATTGGCGCTCCCAATGAGCACACTTAACCACAAAATGA
AGAAGTACGATATTTCTGAAGCGGGTTGA

Upstream 100 bases:

>100_bases
CAGTTACTGCCGCAGCTATCGACACAGCTATTGGTGCATGATAAGGATGATTCACATACGCAAATCGCAGTTTCTGTCGA
TGAGCAAGGAACACTAACCT

Downstream 100 bases:

>100_bases
TAAAAAACGGGAAACGGGAAACGGGAAACGGGAAACGGGAAACGGGAAAATTTTAACTACTGTTTGAACAGAAAAGAATT
AAAAAAACCGCCCGAAGGCG

Product: nitric oxide reductase regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIPSTAHHNDKTIVSHPWEALQI
DSASENDPFNFAIRNGEVVLINELYKYNGYDCESIYQSEQILGLKSENLLAWPLIDSDSKTIGLLVLLDLSVIDNEAALT
EFCRMAASNIRQAVWLEQYGQVIKSLSADNQALVRENTQLKKRTKKTYQGPIAESEEMVNVLNRLDKVLSLPVDVLLRGE
TGAGKEVIAKYIHENSNRADQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFEAANGGTLFLDEIGDMPMLLQ
AKLLRVLQERKVRPIGASKEIEVDVRVIAATHCNLMQQIKDGGFRADLFYRLNVFPITLPPLRERESDIIPLAEHFVQHT
TKTLGLPQAPGLSANVRKQLLAYSYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDTPSVELKAASPTPEQPNYEP
VQADLEDSAIGLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISEAG

Sequences:

>Translated_542_residues
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIPSTAHHNDKTIVSHPWEALQI
DSASENDPFNFAIRNGEVVLINELYKYNGYDCESIYQSEQILGLKSENLLAWPLIDSDSKTIGLLVLLDLSVIDNEAALT
EFCRMAASNIRQAVWLEQYGQVIKSLSADNQALVRENTQLKKRTKKTYQGPIAESEEMVNVLNRLDKVLSLPVDVLLRGE
TGAGKEVIAKYIHENSNRADQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFEAANGGTLFLDEIGDMPMLLQ
AKLLRVLQERKVRPIGASKEIEVDVRVIAATHCNLMQQIKDGGFRADLFYRLNVFPITLPPLRERESDIIPLAEHFVQHT
TKTLGLPQAPGLSANVRKQLLAYSYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDTPSVELKAASPTPEQPNYEP
VQADLEDSAIGLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISEAG
>Mature_542_residues
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIPSTAHHNDKTIVSHPWEALQI
DSASENDPFNFAIRNGEVVLINELYKYNGYDCESIYQSEQILGLKSENLLAWPLIDSDSKTIGLLVLLDLSVIDNEAALT
EFCRMAASNIRQAVWLEQYGQVIKSLSADNQALVRENTQLKKRTKKTYQGPIAESEEMVNVLNRLDKVLSLPVDVLLRGE
TGAGKEVIAKYIHENSNRADQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFEAANGGTLFLDEIGDMPMLLQ
AKLLRVLQERKVRPIGASKEIEVDVRVIAATHCNLMQQIKDGGFRADLFYRLNVFPITLPPLRERESDIIPLAEHFVQHT
TKTLGLPQAPGLSANVRKQLLAYSYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDTPSVELKAASPTPEQPNYEP
VQADLEDSAIGLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISEAG

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=262, Percent_Identity=51.1450381679389, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=44.6808510638298, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788550, Length=356, Percent_Identity=40.1685393258427, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=41.9161676646707, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=49.1596638655462, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=39.8203592814371, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1790437, Length=328, Percent_Identity=39.6341463414634, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=39.4578313253012, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=339, Percent_Identity=41.8879056047198, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786524, Length=317, Percent_Identity=39.1167192429022, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=37.6506024096386, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=36.1607142857143, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=28.6184210526316, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60211; Mature: 60211

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEECC
STAHHNDKTIVSHPWEALQIDSASENDPFNFAIRNGEVVLINELYKYNGYDCESIYQSEQ
CCCCCCCCCEECCCCHHEEECCCCCCCCCEEEEECCCEEEEEHHHHCCCCCHHHHHHHHH
ILGLKSENLLAWPLIDSDSKTIGLLVLLDLSVIDNEAALTEFCRMAASNIRQAVWLEQYG
HCCCCCCCEEEEEEECCCCCCEEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVIKSLSADNQALVRENTQLKKRTKKTYQGPIAESEEMVNVLNRLDKVLSLPVDVLLRGE
HHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
TGAGKEVIAKYIHENSNRADQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
AANGGTLFLDEIGDMPMLLQAKLLRVLQERKVRPIGASKEIEVDVRVIAATHCNLMQQIK
ECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHH
DGGFRADLFYRLNVFPITLPPLRERESDIIPLAEHFVQHTTKTLGLPQAPGLSANVRKQL
CCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LAYSYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDTPSVELKAASPTPEQPNYEP
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
VQADLEDSAIGLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCC
AG
CC
>Mature Secondary Structure
MRSANHSELSNALLAISQSLADRSQLTQTLDAVLTAARQMTLAKHGIIYVLDQTGQALIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEECC
STAHHNDKTIVSHPWEALQIDSASENDPFNFAIRNGEVVLINELYKYNGYDCESIYQSEQ
CCCCCCCCCEECCCCHHEEECCCCCCCCCEEEEECCCEEEEEHHHHCCCCCHHHHHHHHH
ILGLKSENLLAWPLIDSDSKTIGLLVLLDLSVIDNEAALTEFCRMAASNIRQAVWLEQYG
HCCCCCCCEEEEEEECCCCCCEEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVIKSLSADNQALVRENTQLKKRTKKTYQGPIAESEEMVNVLNRLDKVLSLPVDVLLRGE
HHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
TGAGKEVIAKYIHENSNRADQPLIVQNCAAIPEQLLESELFGHKKGSFTGADKDKVGLFE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
AANGGTLFLDEIGDMPMLLQAKLLRVLQERKVRPIGASKEIEVDVRVIAATHCNLMQQIK
ECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHH
DGGFRADLFYRLNVFPITLPPLRERESDIIPLAEHFVQHTTKTLGLPQAPGLSANVRKQL
CCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LAYSYPGNVRELKNIIERSVLLSDFETITHIEFGEQIPEDTPSVELKAASPTPEQPNYEP
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
VQADLEDSAIGLKDVVSQYERTVIIDCLNACNWHTKKAAEQLALPMSTLNHKMKKYDISE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]