Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 156977000

GI number: 156977000

Start: 969588

End: 970577

Strand: Reverse

Name: ddl

Synonym: VIBHAR_05785

Alternate gene names: 156977000

Gene position: 970577-969588 (Counterclockwise)

Preceding gene: 156977001

Following gene: 156976999

Centisome position: 44.04

GC content: 42.73

Gene sequence:

>990_bases
ATGATTAAAAATATTCTTTTACTATGCGGTGGCGGCTCTTCAGAGCACGAGATTTCACTACTTTCTGCAAACTTTGTAGA
GCAACAGCTGAATCTGATTGAAAACGTGAGCGTTACACGCGTAGAAATCAAAAATGAAGGTTGGGTAACAAACCAAGGTG
AATTGGTTTACTTAGACCCTAACCAGAAAAAGCTGTGTTCAGACAACCTAAGCCTAGACGTTGATTTCATTGTCCCTTGT
ATTCATGGTTTCCCTGGCGAAACCGGTGACATTCAATCTATGTTTGAAATCGCGGGCATTCCTTACCTAGGCTGTGGCCC
TGAAGCAAGCAGCAACAGCTTCAACAAAATCACATCTAAGCTTTGGTACGATGCGATTGGCATTCCGAACACTCCATACT
TATTCTTAACACGTAACGATGAGCAAGCTCATGAACAAGCGTTACAAGCGTTTGATAAATGGGGAAAAGTGTTTGTTAAA
GCTGCACGTCAAGGTTCTTCTGTTGGTTGTTACAGTGTCACTAACAAGCAATCTGTTTCCCAAGCTGTGAACGATGCATT
TGGTTACTCAGAGCAAGTCTTGGTAGAAAAGTCAGTCAAACCTCGTGAACTCGAAGTTGCTGCTTATGAGATGAACGGCG
AGTTACACATTACTAAGCCTGGTGAAGTGATTGCGCCTGATGGTGCCTTCTATTCTTACGATGAGAAGTACAGCACGTCT
AGCCATTCACTGACTGAAGTAGAAGCGAAAAACCTGACAGAAGAGCAGGTGGAAGCGATTCGAGTTGCTTCGGAAACCGT
CTTTAAGCAAATGAATTTACGTCATTTATCGCGCATCGACTTCTTCCTAACAGAAGATGGTGAGATTTACCTTAACGAAG
TCAACACCTTCCCAGGAATGACGCCAATCTCGATGTTCCCGAAAATGCTACAAAACAACGGTCACAAGTTCCATGAGTTC
TTAGCAGATTGTATTAATACTGCAATCTAA

Upstream 100 bases:

>100_bases
TATTCGAACAGCATTCCTCACAGTAAAATTACTGTACGTTACTACTCAAAGGTTTATCATACTGAATAGTATCTTTTGGA
GATCACGGAAAGATTGATTC

Downstream 100 bases:

>100_bases
CTAACGTTACCGTCGTTCATTTTGTGGTAAACCTAAGCATAACTGAGTAATGGCACAGTGCTAAACTTTGACTATTACTC
GGTTGCATTTATGATTGTAT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIKNILLLCGGGSSEHEISLLSANFVEQQLNLIENVSVTRVEIKNEGWVTNQGELVYLDPNQKKLCSDNLSLDVDFIVPC
IHGFPGETGDIQSMFEIAGIPYLGCGPEASSNSFNKITSKLWYDAIGIPNTPYLFLTRNDEQAHEQALQAFDKWGKVFVK
AARQGSSVGCYSVTNKQSVSQAVNDAFGYSEQVLVEKSVKPRELEVAAYEMNGELHITKPGEVIAPDGAFYSYDEKYSTS
SHSLTEVEAKNLTEEQVEAIRVASETVFKQMNLRHLSRIDFFLTEDGEIYLNEVNTFPGMTPISMFPKMLQNNGHKFHEF
LADCINTAI

Sequences:

>Translated_329_residues
MIKNILLLCGGGSSEHEISLLSANFVEQQLNLIENVSVTRVEIKNEGWVTNQGELVYLDPNQKKLCSDNLSLDVDFIVPC
IHGFPGETGDIQSMFEIAGIPYLGCGPEASSNSFNKITSKLWYDAIGIPNTPYLFLTRNDEQAHEQALQAFDKWGKVFVK
AARQGSSVGCYSVTNKQSVSQAVNDAFGYSEQVLVEKSVKPRELEVAAYEMNGELHITKPGEVIAPDGAFYSYDEKYSTS
SHSLTEVEAKNLTEEQVEAIRVASETVFKQMNLRHLSRIDFFLTEDGEIYLNEVNTFPGMTPISMFPKMLQNNGHKFHEF
LADCINTAI
>Mature_329_residues
MIKNILLLCGGGSSEHEISLLSANFVEQQLNLIENVSVTRVEIKNEGWVTNQGELVYLDPNQKKLCSDNLSLDVDFIVPC
IHGFPGETGDIQSMFEIAGIPYLGCGPEASSNSFNKITSKLWYDAIGIPNTPYLFLTRNDEQAHEQALQAFDKWGKVFVK
AARQGSSVGCYSVTNKQSVSQAVNDAFGYSEQVLVEKSVKPRELEVAAYEMNGELHITKPGEVIAPDGAFYSYDEKYSTS
SHSLTEVEAKNLTEEQVEAIRVASETVFKQMNLRHLSRIDFFLTEDGEIYLNEVNTFPGMTPISMFPKMLQNNGHKFHEF
LADCINTAI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=355, Percent_Identity=30.7042253521127, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786280, Length=332, Percent_Identity=30.7228915662651, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_VIBHB (A7N512)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001447906.1
- ProteinModelPortal:   A7N512
- SMR:   A7N512
- STRING:   A7N512
- GeneID:   5557281
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_05785
- NMPDR:   fig|338187.4.peg.3405
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 36658; Mature: 36658

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNILLLCGGGSSEHEISLLSANFVEQQLNLIENVSVTRVEIKNEGWVTNQGELVYLDP
CCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCEEEEECC
NQKKLCSDNLSLDVDFIVPCIHGFPGETGDIQSMFEIAGIPYLGCGPEASSNSFNKITSK
CCHHHHCCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
LWYDAIGIPNTPYLFLTRNDEQAHEQALQAFDKWGKVFVKAARQGSSVGCYSVTNKQSVS
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
QAVNDAFGYSEQVLVEKSVKPRELEVAAYEMNGELHITKPGEVIAPDGAFYSYDEKYSTS
HHHHHHCCCCCCEEEECCCCCCEEEEEEEEECCEEEEECCCCEECCCCCEECCCCCCCCC
SHSLTEVEAKNLTEEQVEAIRVASETVFKQMNLRHLSRIDFFLTEDGEIYLNEVNTFPGM
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCC
TPISMFPKMLQNNGHKFHEFLADCINTAI
CHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKNILLLCGGGSSEHEISLLSANFVEQQLNLIENVSVTRVEIKNEGWVTNQGELVYLDP
CCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCEEEEECC
NQKKLCSDNLSLDVDFIVPCIHGFPGETGDIQSMFEIAGIPYLGCGPEASSNSFNKITSK
CCHHHHCCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
LWYDAIGIPNTPYLFLTRNDEQAHEQALQAFDKWGKVFVKAARQGSSVGCYSVTNKQSVS
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
QAVNDAFGYSEQVLVEKSVKPRELEVAAYEMNGELHITKPGEVIAPDGAFYSYDEKYSTS
HHHHHHCCCCCCEEEECCCCCCEEEEEEEEECCEEEEECCCCEECCCCCEECCCCCCCCC
SHSLTEVEAKNLTEEQVEAIRVASETVFKQMNLRHLSRIDFFLTEDGEIYLNEVNTFPGM
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCC
TPISMFPKMLQNNGHKFHEFLADCINTAI
CHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA