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The map label for this gene is tnpA [H]

Identifier: 156976881

GI number: 156976881

Start: 867456

End: 868835

Strand: Reverse

Name: tnpA [H]

Synonym: VIBHAR_05657

Alternate gene names: 156976881

Gene position: 868835-867456 (Counterclockwise)

Preceding gene: 156976882

Following gene: 156976879

Centisome position: 39.42

GC content: 44.42

Gene sequence:

>1380_bases
ATGACACACTCAGACGCTAAACTTTGGGCACAAGAACAATTTGGACAAGCACAACTTAAAGACCCTCGAAGAACACAACG
CCTCATCAGTTTAGCGACTTCTATTGCTAATCAACCAGGAGTCTCCGTGGCTAAACTTCCTTTTTCTCCCGCCGATATGG
AAGGTGCATATCGCTTTATCCGCAATGAAAACATCAATGCAGAAGACATCGCTGAAGCAGGTTTTCAATCAACGGTATCT
CGAGCTAACGAGCATAAGGAATTACTCGCGCTTGAAGATACAACAACGCTCAGCTTTCCGCATCGAAGTATCAAAGAAGA
ACTCGGGCATACTAATCAAGGCGATAGAACTCGAGCTCTCCACGTACATTCGACGTTACTTTTTGCACCACAAAGCCAAA
CCATTGTTGGTCTGATAGAGCAGCAACGATGGAGCCAAGATATTACCAAGCGTGGGCAAAAGCACCAACACGCGACTCGT
CCTTATAAGGAGAAAGAGAGTTACAAGTGGGAGCAAGCCTCTCGCCGTGTCGTTGAACGCCTAGGGGATAAAATGTTAGA
TGTTATCTCGGTCTGTGACCGAGAAGCTGACCTATTCGAATACTTAACTTATAAGCGTCAACATCAGCAGCGTTTTGTCG
TTCGCTCAATGCAAAGTCGTTGTCTAGAAGAGCATGCTCAAAAGCTTTACGACTATGCGCAAGCGTTACCAAGCGTCGAG
ACAAAAGCGCTTACCATCCCCCAAAAAGGAGGGAGAAAAGCGAGAAATGTAAAGCTTGATGTTAAATATGGTCAGGTGAC
ATTAAAGGCTCCGGCAAACAAAAAAGAGCACGCAGGCATACCTGTTTATTATGTTGGTTGTCTTGAGCAAGGGACATCAA
AAGACAAGTTAGCATGGCACCTCTTGACGTCGGAGCCAATAAACAACGTTGAAGATGCCATGAGAATTATCGGTTATTAC
GAGCGCCGATGGCTGATTGAAGATTTCCATAAAGTCTGGAAAAGTGAAGGGACAGATGTGGAGTCACTCAGGCTACAAAG
TAAAGATAACTTAGAGAGATTAAGTGTCATCTATGCCTTTGTTGCTACGCGTTTATTGGCATTACGCTTCATGAAAGAAG
TTGATGAGCTAACGAAAGAAAGTTGTGAAAAGGTATTAGGCCAAAAAGCTTGGAAGCTCCTGTGGTTGAAACTAGAAAGT
AAGACGTTGCCTAAAGAAGTGCCCGATATGGGTTGGGCGTACAAAAATCTCGCTAAATTAGGCGGTTGGAAAGATACCAA
AGGGACGGGTAGAGCTTCAATAAAGGTGCTTTGGGAAGGATGGTTTAAACTACAAACCATCCTTGAGGGCTACGAACTCG
CAATGTCTCTTGATCACTAG

Upstream 100 bases:

>100_bases
CTTATACACAAATCCCCAAGCTTGTAGCTTGGGGATTTTTTTTGAACTCTTTCAGGTGTGTGTGATCTGATCACTTAGCA
ATCCTTACTGAAACACTAAG

Downstream 100 bases:

>100_bases
TCTTGTGATCAAGAGACAGCCAATTAAGGAGGCTTGCAACAACTAATGGGCTAATTACAACTTATCCCAAGCGTCTGACC
AGTAAACGGTTTCACCTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTHSDAKLWAQEQFGQAQLKDPRRTQRLISLATSIANQPGVSVAKLPFSPADMEGAYRFIRNENINAEDIAEAGFQSTVS
RANEHKELLALEDTTTLSFPHRSIKEELGHTNQGDRTRALHVHSTLLFAPQSQTIVGLIEQQRWSQDITKRGQKHQHATR
PYKEKESYKWEQASRRVVERLGDKMLDVISVCDREADLFEYLTYKRQHQQRFVVRSMQSRCLEEHAQKLYDYAQALPSVE
TKALTIPQKGGRKARNVKLDVKYGQVTLKAPANKKEHAGIPVYYVGCLEQGTSKDKLAWHLLTSEPINNVEDAMRIIGYY
ERRWLIEDFHKVWKSEGTDVESLRLQSKDNLERLSVIYAFVATRLLALRFMKEVDELTKESCEKVLGQKAWKLLWLKLES
KTLPKEVPDMGWAYKNLAKLGGWKDTKGTGRASIKVLWEGWFKLQTILEGYELAMSLDH

Sequences:

>Translated_459_residues
MTHSDAKLWAQEQFGQAQLKDPRRTQRLISLATSIANQPGVSVAKLPFSPADMEGAYRFIRNENINAEDIAEAGFQSTVS
RANEHKELLALEDTTTLSFPHRSIKEELGHTNQGDRTRALHVHSTLLFAPQSQTIVGLIEQQRWSQDITKRGQKHQHATR
PYKEKESYKWEQASRRVVERLGDKMLDVISVCDREADLFEYLTYKRQHQQRFVVRSMQSRCLEEHAQKLYDYAQALPSVE
TKALTIPQKGGRKARNVKLDVKYGQVTLKAPANKKEHAGIPVYYVGCLEQGTSKDKLAWHLLTSEPINNVEDAMRIIGYY
ERRWLIEDFHKVWKSEGTDVESLRLQSKDNLERLSVIYAFVATRLLALRFMKEVDELTKESCEKVLGQKAWKLLWLKLES
KTLPKEVPDMGWAYKNLAKLGGWKDTKGTGRASIKVLWEGWFKLQTILEGYELAMSLDH
>Mature_458_residues
THSDAKLWAQEQFGQAQLKDPRRTQRLISLATSIANQPGVSVAKLPFSPADMEGAYRFIRNENINAEDIAEAGFQSTVSR
ANEHKELLALEDTTTLSFPHRSIKEELGHTNQGDRTRALHVHSTLLFAPQSQTIVGLIEQQRWSQDITKRGQKHQHATRP
YKEKESYKWEQASRRVVERLGDKMLDVISVCDREADLFEYLTYKRQHQQRFVVRSMQSRCLEEHAQKLYDYAQALPSVET
KALTIPQKGGRKARNVKLDVKYGQVTLKAPANKKEHAGIPVYYVGCLEQGTSKDKLAWHLLTSEPINNVEDAMRIIGYYE
RRWLIEDFHKVWKSEGTDVESLRLQSKDNLERLSVIYAFVATRLLALRFMKEVDELTKESCEKVLGQKAWKLLWLKLESK
TLPKEVPDMGWAYKNLAKLGGWKDTKGTGRASIKVLWEGWFKLQTILEGYELAMSLDH

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 52854; Mature: 52723

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSDAKLWAQEQFGQAQLKDPRRTQRLISLATSIANQPGVSVAKLPFSPADMEGAYRFI
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
RNENINAEDIAEAGFQSTVSRANEHKELLALEDTTTLSFPHRSIKEELGHTNQGDRTRAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCHHHHHHHHCCCCCCCCEEEE
HVHSTLLFAPQSQTIVGLIEQQRWSQDITKRGQKHQHATRPYKEKESYKWEQASRRVVER
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHHHHHHHHH
LGDKMLDVISVCDREADLFEYLTYKRQHQQRFVVRSMQSRCLEEHAQKLYDYAQALPSVE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKALTIPQKGGRKARNVKLDVKYGQVTLKAPANKKEHAGIPVYYVGCLEQGTSKDKLAWH
CCEEECCCCCCCCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEE
LLTSEPINNVEDAMRIIGYYERRWLIEDFHKVWKSEGTDVESLRLQSKDNLERLSVIYAF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHH
VATRLLALRFMKEVDELTKESCEKVLGQKAWKLLWLKLESKTLPKEVPDMGWAYKNLAKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHH
GGWKDTKGTGRASIKVLWEGWFKLQTILEGYELAMSLDH
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
THSDAKLWAQEQFGQAQLKDPRRTQRLISLATSIANQPGVSVAKLPFSPADMEGAYRFI
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
RNENINAEDIAEAGFQSTVSRANEHKELLALEDTTTLSFPHRSIKEELGHTNQGDRTRAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCHHHHHHHHCCCCCCCCEEEE
HVHSTLLFAPQSQTIVGLIEQQRWSQDITKRGQKHQHATRPYKEKESYKWEQASRRVVER
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHHHHHHHHH
LGDKMLDVISVCDREADLFEYLTYKRQHQQRFVVRSMQSRCLEEHAQKLYDYAQALPSVE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKALTIPQKGGRKARNVKLDVKYGQVTLKAPANKKEHAGIPVYYVGCLEQGTSKDKLAWH
CCEEECCCCCCCCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEE
LLTSEPINNVEDAMRIIGYYERRWLIEDFHKVWKSEGTDVESLRLQSKDNLERLSVIYAF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHH
VATRLLALRFMKEVDELTKESCEKVLGQKAWKLLWLKLESKTLPKEVPDMGWAYKNLAKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHH
GGWKDTKGTGRASIKVLWEGWFKLQTILEGYELAMSLDH
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]