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The map label for this gene is nasB [H]

Identifier: 156976815

GI number: 156976815

Start: 794917

End: 797436

Strand: Direct

Name: nasB [H]

Synonym: VIBHAR_05591

Alternate gene names: 156976815

Gene position: 794917-797436 (Clockwise)

Preceding gene: 156976814

Following gene: 156976816

Centisome position: 36.07

GC content: 49.05

Gene sequence:

>2520_bases
ATGAACAGTCCTTTAACGATTCCAGTAAAGAACGCATTGAGCCACATCGTTGTGGTAGGCAACGGTATGGTCGGGCAACA
CCTTGTCGAGCAATTAGTTCAGAAAAACGCGCATTTTGAGCACCGAATTACCGTGATTGGTGCGGAGCGCTTTATCGCGT
ATGACCGTGTACAGTTGTCCTCTTTGTTTGCGGGTAAATCTCATGATGATTTGATGCTGAGTAACCAAGAATGGTATGAC
ATGCATGGCATCCAACTCGTACTTGGCAGCCAAGTAACAGGCATCGACAGAGAGCAAAAGCGTATTCTGCTTGATGGTGA
AGATATGCTGGGTTACGACCAACTCGTGCTCGCGACCGGTTCTTACCCTTTTGTGCCTCCAATTGAAGGTAAAGACCGAG
ACAACGTGTTTGTCTACCGCACGCTTGATGACCTTTCGCAAATCAAAAAGGCATGTGAAGGCGCAAGAACAGGCGCAGTG
ATTGGCGGCGGCTTGTTGGGTTTAGAAGCGGCAAACGCACTCAAACTATTGGGGGTAGAAACTCATGTTATTGAATTTGC
GCCTCGCCTGATGCCCGTGCAATTAGATGATGGTGCAGGCTTGGTGCTAAAAGAGAAGATTGAAGCGCTAGGGCTAACCG
TTCACACCTCAACGGGTACGGAGCGAATCTGCGATGGTGAAACGGCTAAGCATCGTATGGTGTTTAAAGACAAAGATCCT
CTCGAAGTGGATGTGATTGTCTTTTCCGCAGGCATTCGCCCTCAAGATGAGTTGGCAAGATTGGCCGAGCTAGAAGTCGG
TGAGCGAGGCGGCATTGTGATTAATAACCAATGCCAAACCAGCGATGAGAACATATACGCGATTGGCGAATGTGCCCTTT
GGGAGCAGAAGATCTTTGGTTTAGTCGCACCCGGTTACACCATGGCGAGAACCACCGCCGATGTCTTGACTGGAATGCCG
GGCGAAGGTTTTAAAGGTGCGGACATGAGCACCAAGCTCAAGCTGCTTGGGGTGGATGTGGCGTCTATCGGCGATGCACA
AATGCAAACCGAAGGCGATCAAGAAATGGTGTTGCAAGATGCGGTTTCAGGCATCTACAAAAAGCTGGTGGTTGATGCGA
GCGGCACCCAATTACTAGGTGCGATTCTCGTCGGTGATAACAGCGATTACGATGCGCTCTTACAGTGTTATTTGAACAAA
ACAGTCTTGCCAGACCACGCGGCGAACCTGCTATTTGATACCGGAATGCTGGATGGAGAGATGCCAGACAGCGCCATCAT
CTGTTCTTGTCACAACGTCACAAAAGGTGACTTAGTTGCCGAGATACAAGCTGGAACCACCAACCTGACAGACCTCAAAG
CCAACACCAAAGCCGGTACTGGCTGTGGTGGGTGTAGTAATATGGTGAAGTCGGTATTGGATACTCAACTGGCAGCTATG
GGGGTAGAGCTTAACAACCACCTTTGTGAGCACTTTGAGCTCAGTCGCCAAGAGATGTTCCATATTTGCCAAGTGGAACA
AATCAAAGACTTCGACACCTTAATTGCACTGCATGGCAAGGGGCATGGGTGTGATATCTGTAAGCCGACAGCAGCATCTG
TGTTTGCTTCTTTGTGGAATGAACACATCATGGAGCCTCGACATCAATCTCTGCAAGATTCCAACGATGCCTTTATGGCG
AACCTGCAAAAAGATGGTTCTTACTCGATTGTACCTCGTGTTCCGGGCGGCGAAATTACACCAGACAAACTTATCGTACT
AGGTGAAGTCGCTCAGCAGTATGATCTGTACACCAAGATCACTGGCGGTCAGCGCGTGGATTTATTTGGTGCGCAGTTAG
ATCAACTCCCAGAAATCTGGCAAACCTTAATTGATGCTGGTTTTGAAACAGGGCACGCCTACGGCAAATCGGTGCGCACC
GTGAAATCTTGCGTGGGTTCTACTTGGTGTCGTTATGGTGTTGATGATTCAGTTGGATTAGCCATTGAGCTAGAGAACCG
CTACAAAGGATTGCGAGCGCCCCATAAGCTTAAGTTTGCCGTATCGGGCTGTACTCGCGAGTGTGCAGAAGCGCAAAGTA
AAGACATTGGGGTGATCGCCACCGAAAACGGCTGGAATCTATACGTTTGTGGTAATGGTGGCATGAGACCACGACACGCT
GATTTGTTTGCATCCGACCTATCCAAATCAGAGTTGATAACCATGATCGACCGGGTCTTGATGTTCTACGTCAGCACTGC
TGATCGCTTGCAGCGCACGTCGGTGTGGATGGAGAACATGGAAGGGGGCCTAGACTACCTTAAACAAGTGATTTTGGATG
ACTCTCTGAATCTATGTGACTCTTTGGATGAACAAATGACACGAGTCGTAGATACCTATCAGTGTGAGTGGAAGAGTACG
TTGGATAATCCTCAAAAATTGCAACGCTTCCGTCCTTTCTTGAACAGCACGTCGGGCAGCAAAGTCTTGCCGTACCAGCG
TGTGCGCGGTCAGCGTATTCCAGTGAAACAGGAGGTGTAA

Upstream 100 bases:

>100_bases
GTGTTGAAGTTCCTTTACGAGAAGCAATCCAAAGTCGAGCTTCCTGCTTCGAAAGAAGACAAACCCAAAACCGCAGCGCA
AACGGCATAAGGAGGCAGCG

Downstream 100 bases:

>100_bases
ATGGCAGCGTTAACCAAAGTGAAATTGTGTCAGCTTGATGACTTAATGCCGTTTCTCGGTGCCACTGTATTGATTGAAGG
CGAGCATGTCGCGTTATTCT

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 839; Mature: 839

Protein sequence:

>839_residues
MNSPLTIPVKNALSHIVVVGNGMVGQHLVEQLVQKNAHFEHRITVIGAERFIAYDRVQLSSLFAGKSHDDLMLSNQEWYD
MHGIQLVLGSQVTGIDREQKRILLDGEDMLGYDQLVLATGSYPFVPPIEGKDRDNVFVYRTLDDLSQIKKACEGARTGAV
IGGGLLGLEAANALKLLGVETHVIEFAPRLMPVQLDDGAGLVLKEKIEALGLTVHTSTGTERICDGETAKHRMVFKDKDP
LEVDVIVFSAGIRPQDELARLAELEVGERGGIVINNQCQTSDENIYAIGECALWEQKIFGLVAPGYTMARTTADVLTGMP
GEGFKGADMSTKLKLLGVDVASIGDAQMQTEGDQEMVLQDAVSGIYKKLVVDASGTQLLGAILVGDNSDYDALLQCYLNK
TVLPDHAANLLFDTGMLDGEMPDSAIICSCHNVTKGDLVAEIQAGTTNLTDLKANTKAGTGCGGCSNMVKSVLDTQLAAM
GVELNNHLCEHFELSRQEMFHICQVEQIKDFDTLIALHGKGHGCDICKPTAASVFASLWNEHIMEPRHQSLQDSNDAFMA
NLQKDGSYSIVPRVPGGEITPDKLIVLGEVAQQYDLYTKITGGQRVDLFGAQLDQLPEIWQTLIDAGFETGHAYGKSVRT
VKSCVGSTWCRYGVDDSVGLAIELENRYKGLRAPHKLKFAVSGCTRECAEAQSKDIGVIATENGWNLYVCGNGGMRPRHA
DLFASDLSKSELITMIDRVLMFYVSTADRLQRTSVWMENMEGGLDYLKQVILDDSLNLCDSLDEQMTRVVDTYQCEWKST
LDNPQKLQRFRPFLNSTSGSKVLPYQRVRGQRIPVKQEV

Sequences:

>Translated_839_residues
MNSPLTIPVKNALSHIVVVGNGMVGQHLVEQLVQKNAHFEHRITVIGAERFIAYDRVQLSSLFAGKSHDDLMLSNQEWYD
MHGIQLVLGSQVTGIDREQKRILLDGEDMLGYDQLVLATGSYPFVPPIEGKDRDNVFVYRTLDDLSQIKKACEGARTGAV
IGGGLLGLEAANALKLLGVETHVIEFAPRLMPVQLDDGAGLVLKEKIEALGLTVHTSTGTERICDGETAKHRMVFKDKDP
LEVDVIVFSAGIRPQDELARLAELEVGERGGIVINNQCQTSDENIYAIGECALWEQKIFGLVAPGYTMARTTADVLTGMP
GEGFKGADMSTKLKLLGVDVASIGDAQMQTEGDQEMVLQDAVSGIYKKLVVDASGTQLLGAILVGDNSDYDALLQCYLNK
TVLPDHAANLLFDTGMLDGEMPDSAIICSCHNVTKGDLVAEIQAGTTNLTDLKANTKAGTGCGGCSNMVKSVLDTQLAAM
GVELNNHLCEHFELSRQEMFHICQVEQIKDFDTLIALHGKGHGCDICKPTAASVFASLWNEHIMEPRHQSLQDSNDAFMA
NLQKDGSYSIVPRVPGGEITPDKLIVLGEVAQQYDLYTKITGGQRVDLFGAQLDQLPEIWQTLIDAGFETGHAYGKSVRT
VKSCVGSTWCRYGVDDSVGLAIELENRYKGLRAPHKLKFAVSGCTRECAEAQSKDIGVIATENGWNLYVCGNGGMRPRHA
DLFASDLSKSELITMIDRVLMFYVSTADRLQRTSVWMENMEGGLDYLKQVILDDSLNLCDSLDEQMTRVVDTYQCEWKST
LDNPQKLQRFRPFLNSTSGSKVLPYQRVRGQRIPVKQEV
>Mature_839_residues
MNSPLTIPVKNALSHIVVVGNGMVGQHLVEQLVQKNAHFEHRITVIGAERFIAYDRVQLSSLFAGKSHDDLMLSNQEWYD
MHGIQLVLGSQVTGIDREQKRILLDGEDMLGYDQLVLATGSYPFVPPIEGKDRDNVFVYRTLDDLSQIKKACEGARTGAV
IGGGLLGLEAANALKLLGVETHVIEFAPRLMPVQLDDGAGLVLKEKIEALGLTVHTSTGTERICDGETAKHRMVFKDKDP
LEVDVIVFSAGIRPQDELARLAELEVGERGGIVINNQCQTSDENIYAIGECALWEQKIFGLVAPGYTMARTTADVLTGMP
GEGFKGADMSTKLKLLGVDVASIGDAQMQTEGDQEMVLQDAVSGIYKKLVVDASGTQLLGAILVGDNSDYDALLQCYLNK
TVLPDHAANLLFDTGMLDGEMPDSAIICSCHNVTKGDLVAEIQAGTTNLTDLKANTKAGTGCGGCSNMVKSVLDTQLAAM
GVELNNHLCEHFELSRQEMFHICQVEQIKDFDTLIALHGKGHGCDICKPTAASVFASLWNEHIMEPRHQSLQDSNDAFMA
NLQKDGSYSIVPRVPGGEITPDKLIVLGEVAQQYDLYTKITGGQRVDLFGAQLDQLPEIWQTLIDAGFETGHAYGKSVRT
VKSCVGSTWCRYGVDDSVGLAIELENRYKGLRAPHKLKFAVSGCTRECAEAQSKDIGVIATENGWNLYVCGNGGMRPRHA
DLFASDLSKSELITMIDRVLMFYVSTADRLQRTSVWMENMEGGLDYLKQVILDDSLNLCDSLDEQMTRVVDTYQCEWKST
LDNPQKLQRFRPFLNSTSGSKVLPYQRVRGQRIPVKQEV

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=281, Percent_Identity=26.6903914590747, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI226437568, Length=281, Percent_Identity=26.6903914590747, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI21389617, Length=281, Percent_Identity=26.6903914590747, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789765, Length=809, Percent_Identity=55.5006180469716, Blast_Score=910, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=340, Percent_Identity=26.7647058823529, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=25.4480286738351, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787352, Length=292, Percent_Identity=27.7397260273973, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=287, Percent_Identity=23.3449477351916, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24585130, Length=283, Percent_Identity=24.0282685512367, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281359715, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281359713, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24639250, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI18543267, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24639252, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24639257, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 91947; Mature: 91947

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPLTIPVKNALSHIVVVGNGMVGQHLVEQLVQKNAHFEHRITVIGAERFIAYDRVQLS
CCCCCEEEHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHH
SLFAGKSHDDLMLSNQEWYDMHGIQLVLGSQVTGIDREQKRILLDGEDMLGYDQLVLATG
HHHCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEC
SYPFVPPIEGKDRDNVFVYRTLDDLSQIKKACEGARTGAVIGGGLLGLEAANALKLLGVE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCHHHHCCCH
THVIEFAPRLMPVQLDDGAGLVLKEKIEALGLTVHTSTGTERICDGETAKHRMVFKDKDP
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEECCCCC
LEVDVIVFSAGIRPQDELARLAELEVGERGGIVINNQCQTSDENIYAIGECALWEQKIFG
CEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEECHHHHHHHHHH
LVAPGYTMARTTADVLTGMPGEGFKGADMSTKLKLLGVDVASIGDAQMQTEGDQEMVLQD
EECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCHHCCCCCHHHHHHH
AVSGIYKKLVVDASGTQLLGAILVGDNSDYDALLQCYLNKTVLPDHAANLLFDTGMLDGE
HHHHHHHHHEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHEEECCCCCCC
MPDSAIICSCHNVTKGDLVAEIQAGTTNLTDLKANTKAGTGCGGCSNMVKSVLDTQLAAM
CCCCEEEEEECCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GVELNNHLCEHFELSRQEMFHICQVEQIKDFDTLIALHGKGHGCDICKPTAASVFASLWN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EHIMEPRHQSLQDSNDAFMANLQKDGSYSIVPRVPGGEITPDKLIVLGEVAQQYDLYTKI
HHHCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHCCEEEE
TGGQRVDLFGAQLDQLPEIWQTLIDAGFETGHAYGKSVRTVKSCVGSTWCRYGVDDSVGL
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEE
AIELENRYKGLRAPHKLKFAVSGCTRECAEAQSKDIGVIATENGWNLYVCGNGGMRPRHA
EEEECCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHH
DLFASDLSKSELITMIDRVLMFYVSTADRLQRTSVWMENMEGGLDYLKQVILDDSLNLCD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SLDEQMTRVVDTYQCEWKSTLDNPQKLQRFRPFLNSTSGSKVLPYQRVRGQRIPVKQEV
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNSPLTIPVKNALSHIVVVGNGMVGQHLVEQLVQKNAHFEHRITVIGAERFIAYDRVQLS
CCCCCEEEHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHH
SLFAGKSHDDLMLSNQEWYDMHGIQLVLGSQVTGIDREQKRILLDGEDMLGYDQLVLATG
HHHCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEC
SYPFVPPIEGKDRDNVFVYRTLDDLSQIKKACEGARTGAVIGGGLLGLEAANALKLLGVE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCHHHHCCCH
THVIEFAPRLMPVQLDDGAGLVLKEKIEALGLTVHTSTGTERICDGETAKHRMVFKDKDP
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEECCCCC
LEVDVIVFSAGIRPQDELARLAELEVGERGGIVINNQCQTSDENIYAIGECALWEQKIFG
CEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEEECHHHHHHHHHH
LVAPGYTMARTTADVLTGMPGEGFKGADMSTKLKLLGVDVASIGDAQMQTEGDQEMVLQD
EECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCHHCCCCCHHHHHHH
AVSGIYKKLVVDASGTQLLGAILVGDNSDYDALLQCYLNKTVLPDHAANLLFDTGMLDGE
HHHHHHHHHEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHEEECCCCCCC
MPDSAIICSCHNVTKGDLVAEIQAGTTNLTDLKANTKAGTGCGGCSNMVKSVLDTQLAAM
CCCCEEEEEECCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GVELNNHLCEHFELSRQEMFHICQVEQIKDFDTLIALHGKGHGCDICKPTAASVFASLWN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EHIMEPRHQSLQDSNDAFMANLQKDGSYSIVPRVPGGEITPDKLIVLGEVAQQYDLYTKI
HHHCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHCCEEEE
TGGQRVDLFGAQLDQLPEIWQTLIDAGFETGHAYGKSVRTVKSCVGSTWCRYGVDDSVGL
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEE
AIELENRYKGLRAPHKLKFAVSGCTRECAEAQSKDIGVIATENGWNLYVCGNGGMRPRHA
EEEECCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHH
DLFASDLSKSELITMIDRVLMFYVSTADRLQRTSVWMENMEGGLDYLKQVILDDSLNLCD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SLDEQMTRVVDTYQCEWKSTLDNPQKLQRFRPFLNSTSGSKVLPYQRVRGQRIPVKQEV
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]