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The map label for this gene is artI [H]

Identifier: 156976754

GI number: 156976754

Start: 736716

End: 737459

Strand: Direct

Name: artI [H]

Synonym: VIBHAR_05529

Alternate gene names: 156976754

Gene position: 736716-737459 (Clockwise)

Preceding gene: 156976753

Following gene: 156976755

Centisome position: 33.43

GC content: 44.76

Gene sequence:

>744_bases
ATGAAAAAGATTTTACTAGCTTCACTAATCGGCCTTTCTTCTGCAACCATGGCAGCGAACGCAGCAGCACAAGAAGAGAT
CAAATTCGCGATGGAAGCGACTTACGCACCGTTCGAATACATGGATGAAAACAACCAAATCCAAGGTTTTGACGTGGATC
TGGCGAACGCACTATGTAAAGAAATGGAAGCACAATGTACCTTCCACAACCAATCTTTTGACAGCCTGATCCCTGCATTA
AAATTCAAGCGTTACGATGCGGCTATCTCTGCAATGGATATTACTGACGCGCGTTTGGAGCAAGTGAACTTTTCTGCCCC
TTACTACGACAACTCAGCAGCGTTTGTTTCTATCAAAGAAAAAGTGGCCGATCAAAAAGCACTAGAAGGTAAGCGTGTTG
GTGTTCAAAACGGTTCAACACACCAAAGCTACCTGATCGAGCAAATGCCAGGTGTAACAGCGGTTCCTTACTCAAGCTAC
CAAGACGCATTCATCGATATGAAGAATGGCCGTATTGATTCTGTATTCGGTGACACAGCAGTTGTAGCGGAATGGTTCAA
GAAAGAAAACAACCTAGCGTACGTGGGCGAGCACGTTACTAACCCTAAATACTTCGGTAACGGTTTCGGTATCGCAGTAA
ACAAAGGTAACGACGAGCTAGTGAACAAGCTGAACACGGCACTGAAAACAGTGAAAGCGAACGGCGAATACGACGTAATC
TACAACAAGTACTTCGGTAAGTAA

Upstream 100 bases:

>100_bases
TAAATCACTAAATTACGTTTTTTAAAGTTCAGTCTATTTAACAGATAAGCTCGGCTGTCATTGCGAGTGTCAGCCCACTA
AGTACACACGGAGTATTGCA

Downstream 100 bases:

>100_bases
TATGGCACTAACGGGTTACTCTTTATCGCTTGTTCAAGCTAGTTGGATGACTGTCCAGCTAGCATTCACAAGCCTTTTAG
TCGGTTTGATTCTGGCAGTG

Product: ABC amino acid transporter periplasmic ligand binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKKILLASLIGLSSATMAANAAAQEEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCKEMEAQCTFHNQSFDSLIPAL
KFKRYDAAISAMDITDARLEQVNFSAPYYDNSAAFVSIKEKVADQKALEGKRVGVQNGSTHQSYLIEQMPGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKENNLAYVGEHVTNPKYFGNGFGIAVNKGNDELVNKLNTALKTVKANGEYDVI
YNKYFGK

Sequences:

>Translated_247_residues
MKKILLASLIGLSSATMAANAAAQEEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCKEMEAQCTFHNQSFDSLIPAL
KFKRYDAAISAMDITDARLEQVNFSAPYYDNSAAFVSIKEKVADQKALEGKRVGVQNGSTHQSYLIEQMPGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKENNLAYVGEHVTNPKYFGNGFGIAVNKGNDELVNKLNTALKTVKANGEYDVI
YNKYFGK
>Mature_247_residues
MKKILLASLIGLSSATMAANAAAQEEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCKEMEAQCTFHNQSFDSLIPAL
KFKRYDAAISAMDITDARLEQVNFSAPYYDNSAAFVSIKEKVADQKALEGKRVGVQNGSTHQSYLIEQMPGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKENNLAYVGEHVTNPKYFGNGFGIAVNKGNDELVNKLNTALKTVKANGEYDVI
YNKYFGK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=58.2995951417004, Blast_Score=300, Evalue=4e-83,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=55.1020408163265, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=37.007874015748, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=34.6491228070175, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=33.0677290836653, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=28.6995515695067, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27337; Mature: 27337

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLASLIGLSSATMAANAAAQEEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEECCHHHHHHHHH
EMEAQCTFHNQSFDSLIPALKFKRYDAAISAMDITDARLEQVNFSAPYYDNSAAFVSIKE
HHHHHEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCCEECCCEEEEEEHH
KVADQKALEGKRVGVQNGSTHQSYLIEQMPGVTAVPYSSYQDAFIDMKNGRIDSVFGDTA
HHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCEECCCCCCCCCEEECCCCEECCCCCCHH
VVAEWFKKENNLAYVGEHVTNPKYFGNGFGIAVNKGNDELVNKLNTALKTVKANGEYDVI
HHHHHHHCCCCEEEEECCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
YNKYFGK
ECCCCCC
>Mature Secondary Structure
MKKILLASLIGLSSATMAANAAAQEEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEECCHHHHHHHHH
EMEAQCTFHNQSFDSLIPALKFKRYDAAISAMDITDARLEQVNFSAPYYDNSAAFVSIKE
HHHHHEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCCEECCCEEEEEEHH
KVADQKALEGKRVGVQNGSTHQSYLIEQMPGVTAVPYSSYQDAFIDMKNGRIDSVFGDTA
HHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCEECCCCCCCCCEEECCCCEECCCCCCHH
VVAEWFKKENNLAYVGEHVTNPKYFGNGFGIAVNKGNDELVNKLNTALKTVKANGEYDVI
HHHHHHHCCCCEEEEECCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
YNKYFGK
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]