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The map label for this gene is thlA [H]

Identifier: 156976707

GI number: 156976707

Start: 694184

End: 695395

Strand: Direct

Name: thlA [H]

Synonym: VIBHAR_05482

Alternate gene names: 156976707

Gene position: 694184-695395 (Clockwise)

Preceding gene: 156976701

Following gene: 156976708

Centisome position: 31.5

GC content: 49.83

Gene sequence:

>1212_bases
ATGGGAAACGACATCTGGATTGTCAGTGCAAAGCGAACGCCAATTGGGCGTTTTCAAGGTCAATTACAAGGATATTCAGC
GCCTCAATTAGGGGCATTTGCCATCAAATCGGCAGCGGGTGCGAGCGGGATTAGCCAAGTCGATGAAGTCTATATGGGCT
GCGTATTACCAGCAGGGTGCGGGCAAGCTCCAGCCAGACAAGCGGCGCTAGGTGCAGATCTTCCGCTAACCACAGGCTGT
ACCACCATCAACAAAGTATGTGGATCGGGAATGAAGAGCGTCATGCTGGCTCACGATCTCATCAAAGCGGGTAGTATTCG
AAGTGCTATTGCTGGTGGGATGGAAAGCATGACCAACGCACCGTATCTGCTCAAAGAAGCACGCAACGGCATGCGAATGG
GTCATCAATCTACCTACGACCACATGTTCCTCGATGGTCTTCAAGATGCGTATGAAGGGCATTTGATGGGAGTCTATGCG
CAGCAAACGGCTGACCGTTCTGAGTTTACCCGTGAGCAAATGGATGAATGGGCGATCCTATCCGCGACAAGAGCATTGGA
AGCGCAAGAGCAGCGTCTGTTTGACGATGAAATCTCGCCGATAGAAATCACGACACGTCATAACACTTCAATTTTGGATT
ACGACGAGCATCCTCGCTCGATTAAGCTCGACAAAATTCCTCAACTCAAACCTGCTTTTGATAAGAACGGCTCGGTGACC
GCAGCAAACTCTAGTGCGATTTCTGACGGGGCTGCAGCGTTAGTATTGATGGATTCTCAAGTCGCACAGCATCAAGGCTT
AACGCCATTAGCAATCATCAAAGGGCACTCAACCCACGCCAGAAAGCCTGCTGAATTTACCCTCGCCCCTGTTTTTGCAA
TAGAGCAGTTGTTATCTCAACTCGACTGGTCAGTAGACGAGGTCGATCTTTGGGAGATTAACGAAGCGTTCGCCGTTGTC
ACACAAATCGCGGTACGCCAACTGGGCATCGATAAAGGCAAAGTAAACATCAAAGGCGGTGCGTGTGCGCTTGGGCATCC
AATTGGTGCAAGTGGTGCTCGTATCTTGGTGACTCTCATTCATAGTCTACGCCAACTGCAAACGCTTGGTGTAGATGGTG
ACAACAACAATAAAAAGGTGATGCGAGGTGTTGCATCACTGTGTATCGGTGGTGGTGAAGCCACTGCTATTGGTATCGAG
ATACCGCTTTAA

Upstream 100 bases:

>100_bases
AGAGGCTGGAGTGACAACACACTTCGTGTGAACGCTCCGTGGTAGCAGGTGTGAAGGATGAGTTGTTGAACTGTGAGTTA
TCGTCAAAAGGGACGAAATC

Downstream 100 bases:

>100_bases
TTCCAATTATCAAATAACGTAAAGAAGGACTAGATTATGACTCGCACGGTTCCCTTATTCATCGATGGTGAGTTTCGTGA
ATCTCAAGCAACGGATTGGG

Product: acyl-CoA thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MGNDIWIVSAKRTPIGRFQGQLQGYSAPQLGAFAIKSAAGASGISQVDEVYMGCVLPAGCGQAPARQAALGADLPLTTGC
TTINKVCGSGMKSVMLAHDLIKAGSIRSAIAGGMESMTNAPYLLKEARNGMRMGHQSTYDHMFLDGLQDAYEGHLMGVYA
QQTADRSEFTREQMDEWAILSATRALEAQEQRLFDDEISPIEITTRHNTSILDYDEHPRSIKLDKIPQLKPAFDKNGSVT
AANSSAISDGAAALVLMDSQVAQHQGLTPLAIIKGHSTHARKPAEFTLAPVFAIEQLLSQLDWSVDEVDLWEINEAFAVV
TQIAVRQLGIDKGKVNIKGGACALGHPIGASGARILVTLIHSLRQLQTLGVDGDNNNKKVMRGVASLCIGGGEATAIGIE
IPL

Sequences:

>Translated_403_residues
MGNDIWIVSAKRTPIGRFQGQLQGYSAPQLGAFAIKSAAGASGISQVDEVYMGCVLPAGCGQAPARQAALGADLPLTTGC
TTINKVCGSGMKSVMLAHDLIKAGSIRSAIAGGMESMTNAPYLLKEARNGMRMGHQSTYDHMFLDGLQDAYEGHLMGVYA
QQTADRSEFTREQMDEWAILSATRALEAQEQRLFDDEISPIEITTRHNTSILDYDEHPRSIKLDKIPQLKPAFDKNGSVT
AANSSAISDGAAALVLMDSQVAQHQGLTPLAIIKGHSTHARKPAEFTLAPVFAIEQLLSQLDWSVDEVDLWEINEAFAVV
TQIAVRQLGIDKGKVNIKGGACALGHPIGASGARILVTLIHSLRQLQTLGVDGDNNNKKVMRGVASLCIGGGEATAIGIE
IPL
>Mature_402_residues
GNDIWIVSAKRTPIGRFQGQLQGYSAPQLGAFAIKSAAGASGISQVDEVYMGCVLPAGCGQAPARQAALGADLPLTTGCT
TINKVCGSGMKSVMLAHDLIKAGSIRSAIAGGMESMTNAPYLLKEARNGMRMGHQSTYDHMFLDGLQDAYEGHLMGVYAQ
QTADRSEFTREQMDEWAILSATRALEAQEQRLFDDEISPIEITTRHNTSILDYDEHPRSIKLDKIPQLKPAFDKNGSVTA
ANSSAISDGAAALVLMDSQVAQHQGLTPLAIIKGHSTHARKPAEFTLAPVFAIEQLLSQLDWSVDEVDLWEINEAFAVVT
QIAVRQLGIDKGKVNIKGGACALGHPIGASGARILVTLIHSLRQLQTLGVDGDNNNKKVMRGVASLCIGGGEATAIGIEI
PL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=43.142144638404, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI148539872, Length=405, Percent_Identity=42.2222222222222, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=34.75, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI4501853, Length=408, Percent_Identity=30.8823529411765, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4504327, Length=391, Percent_Identity=27.1099744245524, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194353979, Length=402, Percent_Identity=26.1194029850746, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=46.3840399002494, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=41, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787663, Length=409, Percent_Identity=33.7408312958435, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI48994986, Length=415, Percent_Identity=33.4939759036145, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=27.6699029126214, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=38.5390428211587, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=38.5, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=36.318407960199, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=32.8395061728395, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=27.1889400921659, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325229, Length=409, Percent_Identity=43.0317848410758, Blast_Score=303, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=32.2660098522167, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.142144638404, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=44.139650872818, Blast_Score=313, Evalue=9e-86,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=35.6435643564356, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI17137578, Length=389, Percent_Identity=29.5629820051414, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42915; Mature: 42783

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNDIWIVSAKRTPIGRFQGQLQGYSAPQLGAFAIKSAAGASGISQVDEVYMGCVLPAGC
CCCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GQAPARQAALGADLPLTTGCTTINKVCGSGMKSVMLAHDLIKAGSIRSAIAGGMESMTNA
CCCCHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHCCC
PYLLKEARNGMRMGHQSTYDHMFLDGLQDAYEGHLMGVYAQQTADRSEFTREQMDEWAIL
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
SATRALEAQEQRLFDDEISPIEITTRHNTSILDYDEHPRSIKLDKIPQLKPAFDKNGSVT
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCEE
AANSSAISDGAAALVLMDSQVAQHQGLTPLAIIKGHSTHARKPAEFTLAPVFAIEQLLSQ
ECCCCCCCCCCEEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHH
LDWSVDEVDLWEINEAFAVVTQIAVRQLGIDKGKVNIKGGACALGHPIGASGARILVTLI
HCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCHHHHHHHHH
HSLRQLQTLGVDGDNNNKKVMRGVASLCIGGGEATAIGIEIPL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECC
>Mature Secondary Structure 
GNDIWIVSAKRTPIGRFQGQLQGYSAPQLGAFAIKSAAGASGISQVDEVYMGCVLPAGC
CCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GQAPARQAALGADLPLTTGCTTINKVCGSGMKSVMLAHDLIKAGSIRSAIAGGMESMTNA
CCCCHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHCCC
PYLLKEARNGMRMGHQSTYDHMFLDGLQDAYEGHLMGVYAQQTADRSEFTREQMDEWAIL
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
SATRALEAQEQRLFDDEISPIEITTRHNTSILDYDEHPRSIKLDKIPQLKPAFDKNGSVT
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCEE
AANSSAISDGAAALVLMDSQVAQHQGLTPLAIIKGHSTHARKPAEFTLAPVFAIEQLLSQ
ECCCCCCCCCCEEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHH
LDWSVDEVDLWEINEAFAVVTQIAVRQLGIDKGKVNIKGGACALGHPIGASGARILVTLI
HCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCHHHHHHHHH
HSLRQLQTLGVDGDNNNKKVMRGVASLCIGGGEATAIGIEIPL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]